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Years or even months should be OK. To prevent thawing-freezing damage, it might be helpful to store the plasmid in aliquots according to your needs if you need to store it at -20 degrees Fahrenheit for whatever reason. Additionally, it would remain stable in the fridge. No issues will arise if you store at RT for a month.

You can ship your plasmid to us at room temperature if it is kept in a TE buffer. Otherwise, to lessen the chance of DNA deterioration during shipping, we advise utilizing a cold packaging (blue or dry ice).

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Plasmid DNA can be sent by standard mail by placing 5 ul of the DNA on sterile Whatman filter paper, circling the area with a pen or marker, allowing it to dry, wrapping the filter paper in saran wrap, and sending it.

Sanger sequencing offers the most precise means of confirming the presence of plasmid DNA. Plasmid DNA is extracted from a bacterial culture, and the insert can then be located using sequencing with primers designed for the particular vector.

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