夏凱

夏凱

Kai-Hsia

E-mail: xkay1008@yahoo.com.tw

Research topics

探討Sphingosine-1-Phosphate在構建以幹細胞臍靜脈為基礎的組織工程小口徑血管過程中的作用及其作用機理

自我介紹

Education

1989-1993 Beijing University, Beijing, P. R. C MD Dept. of Medicine

2011-2012 University of Liverpool. The UK MRes Biomedical Science and Translational medicine

Employment

2012- now Taipei Veterans General Hospital, Taiwan Quality director Cryopreserved heart valve and vessel tissue bank

2009- 2011 Taipei Veterans General Hospital, Taiwan Technician Cryopreserved heart valve and vessel tissue bank

2007-2009 HealthBanks Biotech. Co., Ltd, Taipei, Taiwan Senior Researcher R & D Department Stem Cell Research & Protein Vaccine Lab Stem Cell bank

2001-2006 Gene Research Lab. Co., Ltd, Taipei, Taiwan Asistant Manager R & D Department

1999 - 2001 Veterans General Hospital, Taipei, Taiwan Research Assistant Dept. of Surgery

Training Course Certificate

CTBS by AATB (American Association of Tissue Bank)

ISO17025

cGMP

GLP

Professionalism

TISSUE BANK--- Heart valve and cardiovascular processing (processing, preservation, quarantine and storage, distribution), Quality control.

MOL BIOL---DNA or RNA purification, PCR, RT-PCR, DNA cloning, Transformation, Transfection,

BIOCHEM---ELISA, Western blotting, Protein expression (in prokaryote or eukaryote system) and purification(Gel filtration, rProtein A, Sepharose, ion exchange, affinity, HIC, etc.）, Protein refolding

CELL---Basic cell culture skills, Human PBMC separation and proliferation assay, FACScan flow cytometry（cell surface marker stain , DNA stain, intercellular cytokine stain, stem cell QC, Apoptosis test, allergen test of peripheral blood, etc.), polyclone and Monoclonal antibody preparation, MTT assay, drug screenings, apoptosis test, angiogenesis test, MSC reseach. Stem cell culture and differentiations .

Animal's test and Immunology experiment -IHC stain, ELISA development, protein chip, etc)