研究費

2017年度
若手研究者による分野間連携研究プロジェクト (2017-18年度)　(自然科学研究機構)
サイボーグ植物の創出を目指した人工デザイン光合成タンパク質の開発　分担金

2016年度

若手研究者による分野間連携研究プロジェクト (2016年度単年)　(自然科学研究機構)

サイボーグ植物の創出を目指した人工デザイン光合成タンパク質の開発　分担金

2014年度

若手研究(B)  (2014年4月1日~2016年3月31日)
B型肝炎ウイルス複製メカニズムの中心であるDNAポリメラーゼの構造基盤解明

論文

2023年
23. Crystal structure of Campylobacter jejuni lipoprotein Cj1090c.
Paek S, Kawai F, Venisetty N, Kim Y, Yeo HJ.
Proteins. 2023 Mar;91(3):293-299. doi: 10.1002/prot.26434. Epub 2022 Oct 17.

2022年
22. Conformation of Light-Harvesting Complex II Trimer Depends upon Its Binding Site.
Kim E, Kubota-Kawai H, Kawai F, Yokono M, Minagawa J.
J Phys Chem B. 2022 Aug 11;126(31):5855-5865. doi: 10.1021/acs.jpcb.2c04061. Epub 2022 Aug 3.

21. Crystal structure of Tam41 cytidine diphosphate diacylglycerol synthase from a Firmicutes bacterium.
Kimura K, Kawai F, Kubota-Kawai H, Watanabe Y, Tomii K, Kojima R, Hirata K, Yamamori Y, Endo T, Tamura Y.
J Biochem. 2022 Mar 31;171(4):429-441. doi: 10.1093/jb/mvab154.

2019年
20. Single-molecule analysis reveals rotational substeps and chemo-mechanical coupling scheme of Enterococcus hirae V1-ATPase.
Iida T, Minagawa Y, Ueno H, Kawai F, Murata T, Iino R.
J Biol Chem. 2019 Nov 8;294(45):17017-17030. doi: 10.1074/jbc.RA119.008947. Epub 2019 Sep 13.

2018年
19. Plasmid-Based One-Pot Saturation Mutagenesis and Robot-Based Automated Screening for Protein Engineering.
Kawai F, Nakamura A, Visootsat A, Iino R.
ACS Omega. 2018 Jul 31;3(7):7715-7726. doi: 10.1021/acsomega.8b00663. Epub 2018 Jul 11.

18. Chemical array system, a platform to identify novel hepatitis B virus entry inhibitors targeting sodium taurocholate cotransporting polypeptide.
Kaneko M, Futamura Y, Tsukuda S, Kondoh Y, Sekine T, Hirano H, Fukano K, Ohashi H, Saso W, Morishita R, Matsunaga S, Kawai F, Ryo A, Park SY, Suzuki R, Aizaki H, Ohtani N, Sureau C, Wakita T, Osada H, Watashi K.
Sci Rep. 2018 Feb 9;8(1):2769. doi: 10.1038/s41598-018-20987-w.

2017年
17. Cyclosporin derivatives inhibit hepatitis B virus entry without interfering with NTCP transporter activity.
Shimura S, Watashi K, Fukano K, Peel M, Sluder A, Kawai F, Iwamoto M, Tsukuda S, Takeuchi JS, Miyake T, Sugiyama M, Ogasawara Y, Park SY, Tanaka Y, Kusuhara H, Mizokami M, Sureau C, Wakita T.
J Hepatol. 2017 Apr;66(4):685-692. doi: 10.1016/j.jhep.2016.11.009. Epub 2016 Nov 25.

2016年
16. Crystal structure of MytiLec, a galactose-binding lectin from the mussel Mytilus galloprovincialis with cytotoxicity against certain cancer cell types.
Terada D, Kawai F, Noguchi H, Unzai S, Hasan I, Fujii Y, Park SY, Ozeki Y, Tame JR.
Sci Rep. 2016 Jun 20;6:28344. doi: 10.1038/srep28344.

15. Structural insight into photoactivation of an adenylate cyclase from a photosynthetic cyanobacterium.
Ohki M, Sugiyama K, Kawai F, Tanaka H, Nihei Y, Unzai S, Takebe M, Matsunaga S, Adachi S, Shibayama N, Zhou Z, Koyama R, Ikegaya Y, Takahashi T, Tame JR, Iseki M, Park SY.
Proc Natl Acad Sci U S A. 2016 Jun 14;113(24):6659-64. doi: 10.1073/pnas.1517520113. Epub 2016 May 31.

2015年
14. A Novel Tricyclic Polyketide, Vanitaracin A, Specifically Inhibits the Entry of Hepatitis B and D Viruses by Targeting Sodium Taurocholate Cotransporting Polypeptide.
Kaneko M, Watashi K, Kamisuki S, Matsunaga H, Iwamoto M, Kawai F, Ohashi H, Tsukuda S, Shimura S, Suzuki R, Aizaki H, Sugiyama M, Park SY, Ito T, Ohtani N, Sugawara F, Tanaka Y, Mizokami M, Sureau C, Wakita T.
J Virol. 2015 Dec;89(23):11945-53. doi: 10.1128/JVI.01855-15. Epub 2015 Sep 16.

2012年
13. Crystal structure of the Campylobacter jejuni Cj0090 protein reveals a novel variant of the immunoglobulin fold among bacterial lipoproteins.
Paek S, Kawai F, Choi KJ, Yeo HJ.
Proteins. 2012 Dec;80(12):2804-9. doi: 10.1002/prot.24182. Epub 2012 Oct 16.

12. Crystal structures of penicillin-binding protein 3 (PBP3) from methicillin-resistant Staphylococcus aureus in the apo and cefotaxime-bound forms.
Yoshida H, Kawai F, Obayashi E, Akashi S, Roper DI, Tame JR, Park SY.
J Mol Biol. 2012 Oct 26;423(3):351-64. doi: 10.1016/j.jmb.2012.07.012. Epub 2012 Jul 27.

11. Crystal structure of JlpA, a surface-exposed lipoprotein adhesin of Campylobacter jejuni.
Kawai F, Paek S, Choi KJ, Prouty M, Kanipes MI, Guerry P, Yeo HJ.
J Struct Biol. 2012 Feb;177(2):583-8. doi: 10.1016/j.jsb.2012.01.001. Epub 2012 Jan 8.

2011年
10. Structural insights into the glycosyltransferase activity of the Actinobacillus pleuropneumoniae HMW1C-like protein.
Kawai F, Grass S, Kim Y, Choi KJ, St Geme JW 3rd, Yeo HJ.
J Biol Chem. 2011 Nov 4;286(44):38546-38557. doi: 10.1074/jbc.M111.237602. Epub 2011 Sep 9.

2010年
9. A novel intein-like autoproteolytic mechanism in autotransporter proteins.
Tajima N, Kawai F, Park SY, Tame JR.
J Mol Biol. 2010 Oct 1;402(4):645-56. doi: 10.1016/j.jmb.2010.06.068. Epub 2010 Jul 6.

8. Crystallization and preliminary crystallographic studies of the butyrolactone autoregulator receptor protein (BarA) from Streptomyces virginiae.
Yoon YH, Kawai F, Sugiyama K, Park SY, Nihira T, Choi SU, Hwang YI.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Jun 1;66(Pt 6):662-4. doi: 10.1107/S1744309110009930. Epub 2010 May 26.

7. Expression, purification, physicochemical characterization and structural analysis of cytochrome c554 from Vibrio parahaemolyticus strain RIMD2210633.
Akazaki H, Kawai F, Chida H, Hirano T, Hakamata W, Park SY, Nishio T, Oku T.
Biosci Biotechnol Biochem. 2010;74(5):1113-5. doi: 10.1271/bbb.90943. Epub 2010 May 7.

6. Crystal structures of penicillin-binding proteins 4 and 5 from Haemophilus influenzae.
Kawai F, Clarke TB, Roper DI, Han GJ, Hwang KY, Unzai S, Obayashi E, Park SY, Tame JR.
J Mol Biol. 2010 Feb 26;396(3):634-45. doi: 10.1016/j.jmb.2009.11.055. Epub 2009 Dec 1.

2009年
5. Crystallization and structural analysis of cytochrome c(6) from the diatom Phaeodactylum tricornutum at 1.5 A resolution.
Akazaki H, Kawai F, Hosokawa M, Hama T, Chida H, Hirano T, Lim BK, Sakurai N, Hakamata W, Park SY, Nishio T, Oku T.
Biosci Biotechnol Biochem. 2009 Jan;73(1):189-91. doi: 10.1271/bbb.80472. Epub 2009 Jan 7.

2008年
4. Cloning, expression and purification of cytochrome c(6) from the brown alga Hizikia fusiformis and complete X-ray diffraction analysis of the structure.
Akazaki H, Kawai F, Chida H, Matsumoto Y, Hirayama M, Hoshikawa K, Unzai S, Hakamata W, Nishio T, Park SY, Oku T.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Aug 1;64(Pt 8):674-80. doi: 10.1107/S1744309108017752. Epub 2008 Jul 5.

3. The structural basis for an essential subunit interaction in influenza virus RNA polymerase.
Obayashi E, Yoshida H, Kawai F, Shibayama N, Kawaguchi A, Nagata K, Tame JR, Park SY.
Nature. 2008 Aug 28;454(7208):1127-31. doi: 10.1038/nature07225. Epub 2008 Jul 27.

2007年
2. Nickel binding to NikA: an additional binding site reconciles spectroscopy, calorimetry and crystallography.
Addy C, Ohara M, Kawai F, Kidera A, Ikeguchi M, Fuchigami S, Osawa M, Shimada I, Park SY, Tame JR, Heddle JG.
Acta Crystallogr D Biol Crystallogr. 2007 Feb;63(Pt 2):221-9. doi: 10.1107/S0907444906048712. Epub 2007 Jan 16.

2006年
1. Crystal structure of oxidized cytochrome c(6A) from Arabidopsis thaliana.
Chida H, Yokoyama T, Kawai F, Nakazawa A, Akazaki H, Takayama Y, Hirano T, Suruga K, Satoh T, Yamada S, Kawachi R, Unzai S, Nishio T, Park SY, Oku T.
FEBS Lett. 2006 Jun 26;580(15):3763-8. doi: 10.1016/j.febslet.2006.05.067. Epub 2006 Jun 9.