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Majali, I., Qaralleh, H.N., Idid, S.Z., Saad, S., Susanti, D., Althunibat, O.Y. (2015). Potential Antimicrobial Activity of Marine Sponge Neopetrosia exigua. Journal of Basic and Applied Research, 1(1): 1-13
Abstract:
Neopetrosia exigua has received great attention in natural product chemistry. The diversity of N. exigua constituents has been demonstrated by the continued discovery of novel bioactive metabolites such as antimicrobial metabolites. In this study, in order to localise the active component of N. exigua biomass according to the polarity, a sequential gradient partition with different solvents (n-hexane, carbon tetrachloride, dichloromethane, n-butanol, and water) was performed to obtain fractions containing metabolites distributed according to their polarity. The antimicrobial activities of N. exigua fractions were then evaluated using disc diffusion and microdilution methods (influence on the growth curve, Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC)). The results showed that the active metabolites were present in n-hexane, CH2Cl2, n-BuOH, and water fractions. n-hexane, CH2Cl2, and n-BuOH fractions were the most effective fractions. Among microbes tested, Staphylococcus aureus was the most susceptible microbe evaluated. The obtained results are considered sufficient for further study to isolate the compounds represent the antimicrobial activity.Qaralleh, I. (2015). On Dynamics of Quadratic Stochastic Operators. Journal of Basic and Applied Research, 1(1): 14-20
Abstract:
A quadratic stochastic operator (in short QSO) is usually used to present the time evolution of differing species in biology. Some quadratic stochastic operators have been studied by Lotka and Volterra. The general problem in the nonlinear operator theory is to study the behavior of operators. This problem was not fully finished even for quadratic stochastic operators which are the simplest nonlinear operators. To study this problem, several classes of QSO were investigated. We study one class of -QSO defined on 2D simplex. We study the dynamics of thes class by analytic way.Adam, M., Abdull Rasad, M.S.B. (2015). Expression of Matrix Metalloproteinase-13 in Human Skin Melanoma Cancer Treated by Baccaurea angulata in vitro. Journal of Basic and Applied Research, 1(1): 21-28
Abstract:
This study aims to explore the cytotoxicity effects of B. angulata whole fruits and berries as well as to identify the fruit’s probable role on the level of MMP-13 protein expressions in human cancer cells. Cytotoxicity effects of B. angulata were evaluated in vitro using skin melanoma (A375) through treatment with novel B. angulata fibers (whole fruit and berries) via direct contact method. The growth inhibitions of the samples were evaluated through Methylene Blue Assay (MBA) with incubation time of the fibers on cells at 24, 48 and 72 hours. The results showed significant inhibition of growth in all samples with B. angulata whole fruit exhibiting the highest level of inhibition at 72 hours (87.69 %) while its berries showed a reading of 86.16 %. Consequently, this study indicates that both whole fruit fibers and berries of B. angulata may have cytotoxic effects against human skin melanoma. The quantitative expressions of MMP-13 in A375 cell lines which were subjected to the specified amount of fibers were evaluated through ELISA analysis. The results showed no expression of MMP-13 proteins in A375 cells for both whole fruit fibers (-1217.9 pg/ml) and berries (-1222.9 pg/ml). Furthermore, the results of the ELISA analysis depict a probable regulative effect of the fibers toward MMP-13 protein expressions in cancer cells. Hence, it can be concluded that B. angulata fruit has the potential to be used as a new source of natural substitute for anticancer treatment. Moreover, further study is needed in order to find the specific bioactive compounds involved in the anticancer properties which may have been influential in the regulation of MMP-13 proteins that could be fundamental in future endeavors for prospective therapeutic applications.Hroub, N.M. and Brair, S.B. (2015). Patients Satisfaction regarding Quality of Nursing Care in the National Center for Diabetes, Endocrinology and Genetics in Amman, 2013. Journal of Basic and Applied Research, 1(1): 29-35
Abstract:
It is important for any health system to provide high quality health Nursing services and respond to the needs of service users. The aims of this study is to study patients' satisfaction regarding the quality of nursing care of the center, to identify the main dimensions of patients' satisfaction regarding quality of nursing care. And determine the association between sociodemographic variables and patients' satisfaction regarding quality of the nursing care. This study was conducted during the period from1st December 2013 to 1st may 2014. The design of this study is Descriptive cross-sectional facility based study. The study sample 305 patients, who were selected randomly, all of them actually participated in the study and completed face to face interviewed questionnaire prepared according to Likert scale and data was analyzed by SPSS. Participants were moderately satisfied with the quality of nursing care in the National Center for Diabetes Endocrinology and Genetics. The total mean was (3.09), with higher satisfaction levels in the General impressions (M = 3.83) than in Communication, interaction and reaction (M = 2.57). Patients from both settings were less satisfied with the Accessibility of services (M = 2.89). Age, sex, marital status, level of education, income, and the diagnosis show statistically significant effects on patients' level of satisfaction. On the other hand duration of diabetes, and current occupation did not show statistically significant effects on patients' level of satisfaction. The present study concluded that reforming community health nursing care policies to place more attention on increasing the level of communication and the interpersonal aspects of the provided care could probably increase their satisfaction. And the geographical redistribution of community diabetes health center to enable the patient's easy access to nursing services in all Jordan regions, increase number of home visits, for the patients' nursing team need to improve communication, interaction skills of nursing care staff and in formats. Nursing team needed training for nurses on communication skills and interaction with patients and their families.Anthony, VP. and Madu, I. (2015). Survey of the Current Status and Seasonal Problems Associated with Rabbit Production in Kaduna Metropolis. Journal of Basic and Applied Research, 1(1): 36-39
Abstract:
A survey was carried out to ascertain the current status and seasonal problems associated with rabbit production in Kaduna metropolis. Two hundred structured questionnaires were distributed to respondents within the ten study areas as follows: Barnawa, Kurmin-mashi, Kakuri, Kawo,Uguwan,Dosa, Mando, Unguwa-romi, Tudun-wada, Sabon-gari Zaria and Shika Zaria.The following information were sort for from the rabbit farmers: how long they have been involved in rabbits keeping, the type of breeds of rabbits kept, the maximum and minimum number of rabbits ever kept, the reasons for the choice of feeds, availability of the feeds during dry and raining season, problems faced in feeding the rabbits during dry season, common diseases during the two seasons and the advantage of keeping rabbits over other micro livestock. All the data obtained were analyzed using simple frequency distributions. Results shows that there were more male keeping rabbits than female 63.3% and 36.7% respectively. People within the age group of 21-30 years are more into rabbit farming than all other age group. There were more civil servants keeping rabbits than all other profession. 59.20% of the respondents preferred keeping rabbits during wet season when compared to the 40.80% that preferred dry season. Some of the difficulties experienced by farmers include: scarcity of greens/vegetables during dry season, high cost of feed, lack of good storage facilities for hay which does not last throughout the period of scarcity, diseases such as diarrhea and bloat. Possible ways of reducing and controlling these problems were suggested.Page updated
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