Activities
Activities
I) The cell membrane examined by High Speed-AFM
I) The cell membrane examined by High Speed-AFM
Samples: Integral membrane proteins / Membrane-bound proteins / Drug interacting with the cell membrane
Samples: Integral membrane proteins / Membrane-bound proteins / Drug interacting with the cell membrane
Data: Label-free visualisation of individual membrane proteins (structure, dynamics and external stimuli) / Label-free visualisation of the supramolecular environment of the membrane (structure, dynamics and external stimuli) / Mechanical measurements and manipulations using the tip of the AFM
Data: Label-free visualisation of individual membrane proteins (structure, dynamics and external stimuli) / Label-free visualisation of the supramolecular environment of the membrane (structure, dynamics and external stimuli) / Mechanical measurements and manipulations using the tip of the AFM
Results: Physics of the interactions between the membrane proteins, their supramolecular environment and external stimuli (soluble proteins, salinity, pH, force, radiated light) / Biological and medical implications
Results: Physics of the interactions between the membrane proteins, their supramolecular environment and external stimuli (soluble proteins, salinity, pH, force, radiated light) / Biological and medical implications
II) Technical developments
II) Technical developments
-Diamond coated HS-AFM probes
-Diamond coated HS-AFM probes
-Faster HS-AFM
-Faster HS-AFM
-Integration of HS-AFM and Optical Microscopy
-Integration of HS-AFM and Optical Microscopy
III) Emerging lines
III) Emerging lines
- Mechanics of the infectious processes
- Mechanics of the infectious processes
Sample: Salmonella infection of HeLa cells
Data: Mechanical properties of the infected host cells
Results: Salmonella infection affects the mechanical properties of cells
- Enveloped virus: structure-mechanics-dynamics-function
- Enveloped virus: structure-mechanics-dynamics-function
Samples: VSV virus / B. Alphavirus
Data: HS-AFM imaging
Results: Dynamics of assembly and membrane interaction
For more information
https://sites.google.com/view/ignaciocasuso/biological-research
https://sites.google.com/view/ignaciocasuso/technical-development