The experiment for the rabbit animal model consisted of two parts: the detection of the heartbeat (with a single wavelength and fast CCD frame rate); and the detection of the change in blood flow and tissue oxygenation following a vessel occlusion. In the sheep model experiment, no vessels were occluded. In the rabbit experiment, a single laser diode ( = 660 nm) was turned on. The heartbeat of the rabbit ranges from 130 to 220 beats per minute (bpm). Therefore to capture the structure of the contrast change in one pulse period, the frame rate for the rabbit required the value used in the finger test to be doubled. This was not the case with the sheep experiments, where the heart rate is comparable to the human. This, in turn, meant a decrease in the exposure time leading to a fall in the signal intensity and spatial resolution. In order to limit the effects of the latter two, the exposure time was set to 0.5 ms, thus reaching a compromise between the frame rate and the intensity. A total of 30 frames were recorded at a time, the process lasting 1.4 s. In the latter experiment, both laser diodes were used. Data were acquired initially for 25 s, with normal circulation throughout; subsequently the artery was occluded for 25 s while the CCD continued to record images. Finally, the artery was released and the speckle images were recorded for another 15 s. The exposure time was 20 ms and the average frame rate was 1.6 fps for each wavelength. The acquisition time of every frame was also recorded into an Excel document. A customized Labview program controlled and synchronized the laser switch, the filter wheel and the camera acquisition.
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