So i am in the process of setting up our servers for a lan party on my old high school (COVID19 measures in place), i have got Get5 and everything needed to run it and the config of get5 for the match is working.

Get4 and Get5 form a complex. A, yeast lysates were prepared and utilized for immunoprecipitation with anti-Get5. The immunoprecipitates were subjected to gel electrophoresis and then blotted with anti-Get5, anti-Get4, and anti-hexokinase (HxK). Lanes 1, 2, and 3 represent the lysate diluted 1:2000, flow-through diluted 1:2000, and protein bound to antibodies diluted 1:200, respectively. B, the protein immunoprecipitated with anti-Get5 was subjected to gel electrophoresis, and the gel was stained with Coomassie Brilliant Blue. Lanes 1 and 2 represent immunoprecipitates from wild-type and get5 lysates, respectively. C, Get5 co-purifies with His-tagged Get4 using Ni2+-NTA resin. Lanes 1 and 2 represent the soluble fraction of the bacterial lysates and the purified protein, respectively.


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Sgt2 is one of the major proteins associated with the Get4-Get5 complex. However, the role of Sgt2 in the insertion of TA proteins is unclear. On one hand, the phenotypes of sgt2 cells differ from those of get3, get4, and get5 cells. For example, for yeast strains having the S288C genetic background, deletion of GET3, GET4, or GET5 reduces mating efficiency, but deletion of SGT2 does not affect the mating process. It is possible that reduction in mating efficiency for those strains may originate from a defect in TA protein insertion because some TA proteins (e.g. Prm3) are important for the signaling involved in the mating process (34). If so, the lack of an effect of SGT2 deletion on mating efficiency may imply that the product of this gene is not an essential component for the insertion of TA proteins into the ER membrane. On the other hand, Sgt2 may associate with the Get4-Get5 complex in vivo, suggesting that these three proteins may be somewhat interdependent with respect to their various functions. Similarly, chemical genomics analysis results (10) also imply that SGT2 may be functionally linked to GET4 and GET5. Moreover, Sgt2 interacts physically with molecular chaperone Ssa1 (6). Because chaperones may play a role in the insertion of TA proteins into the ER membrane, Sgt2 might also be involved in, but may not be an essential component of, that process.

This is recreation of get5 web panel(Python2.7) with Go and Vue.

Front-end looks pretty same with original get5-web. but API logic is not exactly the same. but most functions should be compatible. ff782bc1db

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