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Gaurav Sharma, Ph.D., M.B.A.
I'm a proud husband, dad of a 3 year old girl and a biomedical research professional committed to improve treatment and diagnosis of metabolic diseases.
Biography
Gaurav Sharma is a biomedical researcher with an expertise in intermediary metabolism and metabolic imaging.
His long-term research interests are the development of magnetic resonance spectroscopy/imaging (MRS/I) techniques for the noninvasive investigation of metabolic processes under both normal and pathologic conditions that can be applied in preclinical and clinical settings. Gaurav's current research focus is mainly in two areas: first, development of novel methods for non-invasive assessment of metabolism and second, understanding the intermediary metabolism in biological systems. In particular, my research involved the use of dynamic nuclear polarization to increase the MR signal of metabolically active, 13C-labeled probes for accessing metabolism in isolated perfused organs and in vivo metabolic imaging, and 13C-NMR isotopomer analysis for assessing in vivo metabolic flux.
His long-term career goal is to be an independent investigator with skills and expertise to find important clinical research problems and their successful outcomes using Magnetic Resonance as a tool. Even very early in his career, he was always intrigued by the biological sciences and its impact on medicine and public health. This inquisitiveness led him to pursue M.S. in biotechnology and later Ph.D. in biomedical science with specialization in Nuclear Magnetic Resonance (NMR). His training in experimental Nuclear Magnetic Resonance (NMR) and biomedical science helped him to develop a unique perspective regarding translational aspects of scientific research. During his graduate training, he focused on metabolomics of anti-cancer drug response, and mastered the experimental high-resolution NMR and MRI techniques.
To accomplish his long-term career goal, he moved to Dallas, Texas and joined Dr. Sherry’s lab at UT Southwestern Medical Center for his postdoctoral training in intermediary metabolism. By using a combination of real-time hyperpolarized 13C-Magnetic Resonance Spectroscopy/Imaging (13C-MRS/I) and 13C isotopomer analysis, he has developed the ability to provide highly sensitive, specific and comprehensive understandings of intermediary metabolism in preclinical rodent model metabolic diseases. Over these years, his graduate and postdoctoral training led a progressive career towards his long- term goal, and he has developed deep understanding and skills in basic metabolism, NMR spectroscopy, hyperpolarization techniques and preclinical hyperpolarized 13C-metabolic imaging as evident by continuous peer reviewed publications, grant support and awards.
WORK
My current research is focus mainly in following two areas:
1. Novel methods for the non-invasive assessment of metabolism: Hyperpolarized 13C MR spectroscopy (MRS) using dynamic nuclear polarization (DNP) is an emerging technique to noninvasively assess mitochondrial metabolism in real-time. I have been investigating the altered in-vivo and ex-vivo real-time metabolism in rodent disease models and perfused isolated organs using hyperpolarized 13C MRS/I.
2. Understanding the intermediary metabolism in biological systems: The vast majority of diseases are associated with disruption of metabolic pathways. Tracing intermediary metabolism with stable isotope infusions/perfusion is safe and highly informative in rodents and humans. I have been currently involved in the understanding role of altered metabolism in rodent disease models and perfused organ using 13C isotopomer and flux analysis.
AFFILIATIONS
UT Southwestern Medical Center, Dallas, TX, USA
Postdoctoral Researcher Summer 2016-till date
•Successfully established the novel Hyperpolarized 13C Magnetic Resonance Imaging (13CMRS/I) for the noninvasive invivo metabolic assessment of Hibernating myocardium in rat model and liver in Pyruvate Dehydrogenase Kinase (PDK) knockout mice.
•Performed investigations to understand the metabolism in biological systems using 13C Isotopomer and metabolic flux analysis.
•Developed a rat model of Hibernating myocardium, that is, myocardium supplied by a coronary with a flow-limiting lesion that recovers after revascularization.
•Led the investigations of altered metabolic state in diseases conditions in perfused isolated organs (including heart, liver and kidneys) using hyperpolarized 13C MRS.
•Supervised the dissertations of STARS (Science Teacher Access to Resources at Southwestern) fellows.
•Assisted the development of 13C isotopmer and metabolic flux analysis tool ‘tcaCALC’ and 13C labelling in intermediary metabolism simulation tool ‘tcaSIM’.
•Established and successfully accomplished several collaborative projects in intermediary metabolism and hyperpolarized 13C metabolic imaging.
Jawaharlal Nehru University, New Delhi, India
Research Associate Spring 2016–Summer 2016
Senior Research Fellow Summer 2015–Spring 2016
•Developed NMR module in Computational Core for Plant Metabolomics (CCPM) for the metabolite identification and data analysis of plant metabolomics data.
•Established two NMR projects in CCPM for the testing, debugging and demonstration of NMR module. Currently serving as PI/advisor for this project .
•Maintain and facilitate deployment of CCPM server at JNU for this inter-institutional project.
•Successfully organized the workshop and developed the tutorials for CCPM users.
All India Institute of Medical Sciences, New Delhi, India
Senior Research Fellow Summer 2012–Summer 2015
Junior Research Fellow Summer 2010–Summer 2012
•Experimentally validated the polyherbal formulations indicated and used for the treatment cancer in Indian traditional medicine system (Ayurveda).
•Standardized the methods for the in-depth spectroscopic (NMR, FTIR and LCMS) characterization and pharmacological evaluation of select polyherbal formulations.
•Developed the methods for NMR based metabolic assessment of drug response for anticancer and antiangiogenic potential of polyherbal formulations.
•Successfully established the pharmacological taste grading of polyherbal formulations (first in India) using Alpha MOS E-tongue system.
•Co-supervised the dissertations of two MS biotechnology (AIIMS) students .
National JALMA Institue of Leprosy & Other Mycobacterial diseases (ICMR), Agra, India
Dissertation Trainee Spring 2009–Fall 2009
•Analyzed differentially expressed proteins and sero-reactivity of M. tuberculosis clinical isolates as potential vaccine candidates
•Standardized the methods for the potential vaccine candidate identification and assisted the MALDI-TOF data acquisition and analysis for sero-reactive proteins.
Institute of Nuclear Medicine & Allied Sciences (INMAS), New Delhi, India
Research Intern Summer 2008-Fall 2008
•Evaluated the cell lines and rodent tumor models with different dose of radiations (Gy).
•Assisted scientists in experiments to evaluate DNA damage and RNA expressions following radiation dose in cells.
EDUCATION
Master of Business Administration - MBA (Business Administration, Leadership, Management and Entrepreneurship)
Quantic School of Business and Technology
July 2019 - July 2020
o Successfully completed a highly selective accredited MBA curriculum developed with business professors from the top MBA programs delivered via Quantic's revolutionary active learning platform.
o Advanced leadership, management, business and entrepreneurship skills through several collaborative case studies, discussions, and group projects.
o Effectively honed networking and communication skills through a professional network of Quantic alumni from diverse backgrounds from around the world.
o Developed insights into the identification and execution of business opportunities by learning from peers and business leaders.
Doctor of Philosophy-PhD (NMR/ Biomedical Science)
All India Institute of Medical Sciences, New Delhi, India
July 2010 - March 2016
Department of Nuclear Magnetic Resonance Imaging (NMRI)
Thesis title: NMR spectroscopic analysis and anticancer evaluation of select ayurvedic polyherbal formulations
Advisors: Rama Jayasundar, PhD (Professor and Head, NMRI); S.S. Chauhan, PhD (Professor and Head, Biochemistry); T. Velpandian, PhD (Professor and Head, Ocular Pharmacology); and Dr. Uma Sharma (Associate Professor, NMRI)
School of Life Sciences (SLS)
Thesis Title: Analysis of differentially expressed proteins and sero-reactivity of M. tuberculosis clinical isolates in comparison to lab strain H37Rv
Advisor: Dr. Beenu Joshi, Deputy Director/ Scientist F, National JALMA Institute of Leprosy & other Mycobacterial Diseases [ICMR], Agra, Uttar Pradesh, India.
Agra College
Subjects: Chemistry, Zoology and Botany
PUBLICATIONS
18. Singh S, Suh EH, Sharma G, Chen J, Hackett EP, Wen X, Sherry AD, Khemtong C,. Malloy CR, Park JM, and Kovacs Z. 13C-enriched diethyl ketoglutarate derivatives as hyperpolarized probes of TCA cycle activity. RSC Chemical Biology, 2021
17. Dai C, Xiao X, Yuan Y, Sharma G, Tang SS. A comprehensive toxicological assessment of fulvic acid. Evid Based Complement Alternat Med. 2020; 2020:8899244. PMID: 33381216 doi:10.1155/2020/8899244. PMID: 33381216
16. Dai C, Li Q, Herman I. May, Sharma G, Zhang G, Deng Y, Sherry AD, Malloy CR, Khemtong C, Gillette TG, Scadden DT, Wang ZV. Lactate dehydrogenase A governs cardiac hypertrophic growth in response to hemodynamic stress. Cell Reports. 2020. PMID: 32877669. https://doi.org/10.1016/j.celrep.2020.108087, PMID: 32877669, PMCID: PMC7520916
15. Dai C, Lui Q, Li D, Sharma G, Tang S, Xiaog J, Xiao X. Molecular insights of copper sulfate exposure-induced nephrotoxicity: involvement of oxidative and endoplasmic reticulum stress pathways. Biomolecules. 2020. PubMed PMID: 32650488 DOI: https://doi.org/10.3390/biom10071010
14. Dai C, Wang Y, Sharma G, Shen J, Velkov T, Xiao X. Polymyxins-curcumin combination antimicrobial therapy: safety implications and efficacy for infection treatment. Antioxidants. 2020. DOI: https://doi.org/10.3390/antiox9060506 PubMed PMID: 32526966
13. Cardoso AC, Savla JJ , Lam NT , Nakada Y, Pereira AHM, Elnwasany A , Menendez-Montes I , Ensley E, Petric UB , Sharma G, Sherry AD, Malloy CR, Khemtong C, Kinter M, Ten WLW, Anene-George CG, Abdisalaam S, Asaithamby A, Xing C , Kanchwala M , Vale G, Eckert KM, Mitsche M, McDonald JG, Hill J, Szweda IL, Sadek AS. Mitochondrial Substrate Utilization Regulates Cardiomyocyte Cell Cycle. Nature Metabolism. 2020. https://doi.org/10.1038/s42255-020-0169-x Pubmed PMID: 32617517 PMCID: PMC7331943
12. Sharma G, Wu CY, Wynn RM, Gui W, Malloy CR, Sherry AD, Chuang DT, Khemtong C. Real-time hyperpolarized 13C magnetic resonance detects increased pyruvate oxidation in PDK2/4 double knockout livers. Scientific Reports. 2019. https://doi.org/10.1038/s41598-019-52952-6 PubMed PMID: 31712597
11. Chen W, Sharma G#, Jiang W, Meptune NR, Malloy CR, Sherry AD, Khemtong C. Metabolism of hyperpolarized 13C-acetoacetate to β- hydroxybutyrate detects real-time mitochondrial redox state and dysfunction in heart tissue. NMR in Biomedicine. 2019. #Equal first author https://doi.org/10.1002/nbm.4091 PubMed PMID: 30968985
10. Sharma G, Chen W, Jiang W, Anderson AL, Malloy CR, Sherry AD, Khemtong C. Response of ischemic myocardium to adrenergic stimulation as detected by hyperpolarized [1-13C]pyruvate. Circulation Research. 2019;125:A565 doi: https://doi.org/10.1161/res.125.suppl_1.565
9. Maptue NR, Jiang W, Harrison C, Funk AM, Sharma G, Malloy CR, et al. Esterase-catalyzed production of hyperpolarized 13 C-enriched carbon dioxide in tissues for measuring pH. ACS Sensors. 2018. Nov 6;acssensors.8b01097. https://doi.org/10.1021/acssensors.8b01097 PubMed PMID: 30398335
8. Singh S, Suh EH, Sharma G, Khemtong C, Sherry AD, Kovacs Z. Probing carbohydrate metabolism using hyperpolarized 13C-labeled molecules. NMR in Biomedicine. 2018. doi: https://doi.org/10.1002/nbm.4018 PubMed PMID: 30474153
7. Makarewich CA, Baskin KK, Munir AZ, Bezprozvannaya S, Sharma G, Khemtong C, Shah AM, McAnally JR, Malloy CR, Szweda LI, Bassel-Duby R, Olson EN. MOXI Is a Mitochondrial Micropeptide That Enhances Fatty Acid β-Oxidation. Cell Reports. 2018; 23(13):3701-9. doi: https://doi.org/10.1016/j.celrep.2018.05.058. PubMed PMID: 29949755
6. Wu CY, Satapati S, Gui W, Wynn RM, Sharma G, Lou M, Qi X, Burgess SC, Malloy C, Khemtong C, Sherry AD, Chuang D, Merritt ME. A Novel Inhibitor of Pyruvate Dehydrogenase Kinase Stimulates Myocardial Carbohydrate Oxidation in Diet-Induced Obesity. J Biol Chem. 2018. Epub 2018/05/10. doi: https://doi.org/10.1074/jbc.RA118.002838. PubMed PMID: 29739849.
5. Wu CY, Tso SC, Chuang JL, Gui WJ, Lou M, Sharma G, Khemtong C, Qi X, Wynn RM, Chuang DT. Targeting hepatic pyruvate dehydrogenase kinases restores insulin signaling and mitigates ChREBP-mediated lipogenesis in diet-induced obese mice. Mol Metab. 2018. Epub 2018/04/16. doi: https://doi.org/10.1016/j.molmet.2018.03.014. PubMed PMID: 29656110.
4. Sharma G, Pandey S, Ghatak S, Watal G, Rai PK. Potential of Spectroscopic Techniques in the Characterization of “Green Nanomaterials”. Nanomaterials in Plants, Algae, and Microorganisms. Elsevier Inc. 2018. p. 59-77.
3. Sharma G, Jayasundar R, Chauhan SS, Velpandian T. Therapeutic potential of anticancer polyherbal formulations. Molecular Cancer Therapeutics. 2013, C108, 12, 11. doi: https://doi.org/10.1158/1535-7163.TARG-13-C108
2. Watal G, Watal A, Rai PK, Rai DK, Sharma G, Sharma B. Biomedical applications of nano-antioxidant. Methods Mol Biol. 2013;1028:147-51. Epub 2013/06/07. doi: 10.1007/978-1-62703-475-3_9. PubMed PMID: 23740118.
1. Jaiswal D, Rai PK, Mehta S, Chatterji S, Shukla S, Rai DK, Sharma G, Sharma B, Khair S, Watal G. Role of Moringa oleifera in regulation of diabetes-induced oxidative stress. Asian Pac J Trop Med. 2013;6(6):426-32. Epub 2013/05/29. doi: 10.1016/S1995-7645(13)60068-1. PubMed PMID: 23711700.
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