Plant tissue culture is a collection of techniques used to maintain or grow plant cells, tissues or organs under sterile conditions on a nutrient culture medium of known composition. It is widely used to produce clones of a plant in a method known as micropropagation. It relies on the ability of plant cells to regenerate a whole plant (totipotency). Single cells, plant cells without cell walls (protoplasts), pieces of leaves, stems or roots can often be used to generate a new plant on culture media given the required nutrients and plant hormones.

The module is composed with sub-titles such as Benefits of Plant Tissue Culture; Equipment for Plant Tissue culture; General Requirements for Growing Tissue in Culture; Types of Plant Tissue Culture; Cryopreservation and Germplasm Storage; Rapid Clonal Propagation; and Production of Secondary Metabolities by Cell Culture