Many months later, one of my good chess.com friends @ZionPureinHeart mentioned how he was working towards a goal of his to reach 50+ score in Puzzle Rush Survival. I encouraged him and thought it was a nice goal to aim for, but that was as far as that conversation went.

Making things worse for my progress was that I assumed my ability was mid 40s since I scored 46 on my first ever attempt. The truth was much harsher. For months, I struggled to even pass 30 and 40+ was a rare occurrence. Obviously my 46 was hard-earned, but not representative to what I could consistently score on average. Slowly, yet surely, I was making progress; but it took longer than I expected.

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Another good chess.com friend of mine (and also a friend in real life) @Arrakis09 generously gifted me a chess.com gold membership for reasoning unrelated to my puzzle rush ambitions. This changed my Puzzle Rush Survival routine because now I was allotted five Puzzle Rush attempts per day instead of the one per day I was previously working with! Literally overnight, I now had access to five times as many attempts and this consequently means five times as much Survival! The renewed hope would be that a month or two of hard work and I could finally reach a score of 50+ by simply putting in more volume (note that is what I also thought the first time around months prior).

When I used to use one Survival attempt per day, I was also playing lots of chess games, habitually working on my daily tactics and studying chess on the side. My chess was fairly balanced and Survival was just one major component. However, one Survival run might take me an hour or two to reach 30 puzzles correct (at that point in time anyway). This was a lot, but it was manageable. The problem now was mental fatigue and literally scheduling. Assuming one good Survival attempt took me two hours, that would take me ten hours per day if I were to have deep runs on all five of my daily attempts!

I admire GM Bobby Fischer for being able to invest so much effort into chess, but there is no doubt that chess was predominantly his life. It is not the same for me to this extent. I still have school and other hobbies; I was not coming across ten hours for Survival that easily.

The fictional story is of two hardworking lumberjacks who each were cutting down a tree with an axe. The first lumberjack kept chopping at the tree trunk when he noticed his axe blade getting dull. He made up his mind to sharpen the blade when he finished cutting down the tree and kept hacking away for the time being.

The second lumberjack also noticed his axe blade getting dull from the cutting, but he arrived at a different conclusion. The second lumberjack decided to temporarily pause his progress on cutting down the tree and use that time to sharpen his blade.

I instead began playing Survival a lot slower and not necessarily utilizing all five attempts per day just because I had them available. Doing so would just burn me out and carry into the next day, so instead I would only play Survival two or three times a day and I was getting deep runs still about once a day. This means that I was pushing my high scores even though I was actually playing slower and less attempts than before!

Now I was beginning to score 40s more often and then I got a new personal best of 47 about a month or two later! After some more hard work, I reached 48 and even 49 once or twice about a month or so after that.

By chance, it was just a few seconds after I get this puzzle incorrect that my chess.com friend @Anatomy99 messages me in Connect Messages. We talk for a little bit (mostly about my current Survival progress) and then I minimize the messages and go back to solving my puzzles.

After this grueling Survival attempt lasting so long, I can only wonder if I would have failed to reach my goal if I was only a smidge slower calculating and had the internet go out when I was on 49 or something instead of just after completing 50 and finally going out entirely after 52.

For whatever reason, my internet finally comes back on about an hour later (I was keeping tabs via my phone if and when the internet returned). I log back onto chess.com and by chance @Anatomy99 is still online. I message them, since our conversation was abruptly cut when my Connect Messages went out on puzzle 50. I tell them this story of my internet issues and what had happened. We chat for a few minutes more and then we decide to part ways until morning.

I also want to review the puzzles I got incorrect in that run too, but there is no way to find them again. The chess.com stats have no record of the attempt and indirectly no record of my incorrect puzzles.

It would be nice to have a little more closure to reaching the goal that has taken me about one year to complete, and having it correctly recorded in the profile stats, but at least I know I reached it.

So, dear reader, do you believe a truly great horror game needs to have its share of puzzles? Or do you think the genre has outgrown this aging trope? We love to hear what you think, so be sure to sound off in the comments and let us know.

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Durable humoral immunity is dependent upon the generation of antigen-specific antibody titers, produced by non-proliferating bone marrow resident long-lived plasma cells (LLPC). Longevity is the hallmark of LLPC, but why and how they survive and function for years after antigen exposure is only beginning to be understood. LLPC are not intrinsically long-lived; they require continuous signals from the LLPC niche to survive. Signals unique to LLPC survival (vs. PC survival in general) most notably include those that upregulate the anti-apoptotic factor Mcl-1 and activation of the CD28 receptor expressed on LLPC. Other potential factors include expression of BCMA, upregulation of the transcription factor ZBTB20, and upregulation of the enzyme ENPP1. Metabolic fitness is another key component of LLPC longevity, facilitating the diversion of glucose to generate pyruvate during times of stress to facilitate long term survival. A third major component of LLPC survival is the microenvironment/LLPC niche itself. Cellular partners such as stromal cells, dendritic cells, and T regulatory cells establish a niche for LLPC and drive survival signaling by expressing ligands such as CD80/CD86 for CD28 and producing soluble and stromal factors that contribute to LLPC longevity. These findings have led to the current paradigm wherein both intrinsic and extrinsic mechanisms are required for the survival of LLPC. Here we outline this diverse network of signals and highlight the mechanisms thought to regulate and promote the survival of LLPC. Understanding this network of signals has direct implications in increasing our basic understanding of plasma cell biology, but also in vaccine and therapeutic drug development to address the pathologies that can arise from this subset.

PNS has had stellar movement in the rankings: when looking at US iPhone top-grossing ranks for all games, PNS entered the top 100 less than three months after launch and continued to rise up the ranks to break into the highly coveted top 10 grossing spot and staying there since May 2022. As for the download ranks, the game has maintained a steady top 150-250 ranks in games overall and top 25 in strategy games.

Given its remarkable performance in the environment of IDFA depreciation, which hit the 4X genre relying on finding a small audience of high-spending whales quite hard, and its mastery of combining the hardcore genre of 4X with the more casual Match 3 RPG, this essay deep dives into the hows and whys of PNS:

When players train units and March-battle on the map for resources, they can lose Might for losing troops in battle. When player troops engage enemies on the map, mainly PvE in the beginning, an instant battle report is generated depending on the difference in power and types of the two armies. This March-battle system has a lot of depth when it comes to optimizing battle performance and is quite complicated to learn initially before getting the hang of all the underlying systems. The lack of visuals simulating the battle also makes it hard to imagine how these are playing out and adds to the steep learning curve.

PNS starts off players with the base building and upgrading basics but diverts the experience from there into a more traditional hero collector puzzle RPG. Players are introduced to the gacha machine where they pull heroes from, with their rarity ranging from 1 to 5 stars, make a team, and pursue a PvE campaign of Match 3 battles. The rewards from the campaign fuel the growth of the heroes, upgrades of the base, and research that improves both the Match 3 and 4X aspects. The game also deeply monetizes these puzzle RPG features, primarily through the hero gacha machine where players can pay to acquire stronger heroes.

The power growth and strategy of the early game puzzle Match 3 RPG acts as a steady introduction to the more strategic gameplay of the late game. Players are smoothly transitioned from the PvE, single-player features of the puzzle match-3 battles to the PvP, multiplayer features of the 4X march-battles, naturally encountering other players on the map and nudging them into joining alliances for survival in the unforgiving zombie apocalypse. This helps less hardcore strategy players get enough experience in the game before transitioning to the more hardcore strategic 4X war elements.

This synergy between the advertising and in-game elements helps funnel players into a cohesive experience that keeps them engaged and acts as a feedback loop for the advertising to be effective in getting players. This approach shows a strong synergy between the game and UA teams, effectively building and sharing ideas that makes the advertising as well as the game product more effective. 152ee80cbc

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