Emerging tools for single-cell biology: microfluidics approaches for high-throughput omics profilingÂ
Emerging tools for single-cell biology: microfluidics approaches for high-throughput omics profilingÂ
by D. Zhu, K. Natarajan
In this review article of "emerging tools for single-cell biology", I systematically summarized the significant role of microfluidics as a precision tool in modern biology. I discussed different techniques of how microfluidics exploits miniaturized dimensions and unique features of microscopic flow (such as laminar streams, concentration gradients, monodisperse emulsions) for perturbing systems with extraordinary control. A particular focus on single-cell studies of genomics, epigenomics, transcriptomics, proteomics, and metabolomics with proper microfluidic tools was reviewed. In addition, I discussed the current and future impact of microfluidic technology in the fields of developmental biology and microbiol ecology, alongside the constrains to most biological labs in terms of materials and facbrication techniques (lithography, 3D printing, etc). Further spreading of the technology and innovations will enable microfluidics growingly advancing modern biology studies in a more controllable and quantitative manner.