HOBBIES & INTERESTS
Basketball, physical fitness, sauna/spa, travel, movie, anime, science, business, adventure, beautiful views, communication with people
EDUCATION
Ph.D. (April 2020 – March 2024)
Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo, Japan
Research title: Whole-body and whole-organ imaging of hypoxia using fluorescent molecular probe compatible with tissue clearing
Supervisor: Prof. Shinsuke Sando
M.Eng. (April 2018 – March 2020)
Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo, Japan
Supervisor: Prof. Shinsuke Sando
B.Sc. (April 2014 – March 2018)
Chemistry Course, College of Science, Ibaraki University, Japan
Research title: Development of a green and sustainable synthetic method of thiazole by the dehydration condensation/cycloisomerization reaction of propargyl alcohol and thioamide
Supervisor: Associate Prof. Hirotaka Kagoshima
PUBLICATIONS
I. Tamura, D. M. Sakamoto, B. Yi, Y Saito, N. Yamada, J. Morimoto, Y. Takakusagi, M. Kuroda, S. I. Kubota, H. Harada, K. Tainaka, and S. Sando "Click3D: Click reaction across deep tissues for whole-organ 3D fluorescence imaging" Sci. Adv., 2024, 10, eado8471.
I. Tamura, D. M. Sakamoto, B. Yi, Y. Saito, N. Yamada, Y. Takakusagi, and S. Sando "Pimonidazole-alkyne conjugate for sensitive detection of hypoxia by Cu-catalyzed click reaction"Anal. Sci., 2024, 40, 1061-1070. https://link.springer.com/article/10.1007/s44211-024-00520-y
D. M. Sakamoto, I. Tamura, B. Yi, S. Hasegawa, Y. Saito, N. Yamada, Y. Takakusagi, S. I. Kubota, M. Kobayashi, H. Harada, K. Hanaoka, M. Taki, M. Nangaku, K. Tainaka, and S. Sando "Whole-body and whole-organ 3D imaging of hypoxia using an activatable covalent fluorescent probe compatible with tissue clearing" ACS Nano, 2024, 18, 5167-5179. https://pubs.acs.org/doi/full/10.1021/acsnano.3c12716
R. Nakahara, S. Aki, M. Sugaya, H. Hirose, M. Kato, K. Maeda, D. M. Sakamoto, Y. Kojima, M. Nishida, R. Ando, M. Muramatsu, M. Pan, R. Tsuchida, Y. Matsumura, H. Yanai, H. Takano, R. Yao, S. Sando, M. Shibuya, J. Sakai, T. Kodama, H. Kidoya, T. Shimamura, and T. Osawa “Hypoxia activates SREBP2 through golgi disassembly in bone marrow-derived cells for tumorigenesis” EMBO J., 2023, e114032. https://doi.org/10.15252/embj.2023114032
CONFERENCE PRESENTATIONS
The 17th Symposium on Biorelevant Chemistry, Chiba, Japan, September 2023.
Pacifichem 2021, Online, Dec. 2021.
The 11th CSJ Chemistry Festa, Online, Japan, October 2021.
The 15th Symposium on Biorelevant Chemistry, Online, Japan, September 2021.
The Chemical Society of Japan, 101st Annual Meeting, Online, Japan, March 2021.
The 8th CSJ Chemistry Festa, Funabashi, Japan, October 2018.
EMPLOYMENT EXPERIENCE
Postdoctoral Research Associate, The Conway group, University of California, Los Angeles (employed by the University of Oxford) (April 2024 – Present)
Research assistant, Sando laboratory, The University of Tokyo (April 2020 – March 2024)
Research assistant, Institute for Open Innovation, The University of Tokyo (April 2023 – June 2023)
Research assistant, Graduate School of Engineering, the University of Tokyo Doctoral Student Special Incentives Program (April 2020 – March 2024)
Teaching assistant, First-Year Seminar, The University of Tokyo (April 2020 – March 2021)
HONORS & AWARDS
Grant-in-aid from The Toyobo Biotechnology Foundation, 2023. (declined)
Outstanding Candidates, Research assistant, Graduate School of Engineering, the University of Tokyo Doctoral Student Special Incentives Program, 2022.
Best Poster Award, The 11th CSJ Chemistry Festa, Online, Japan, October 2021. (Winner’s comment in Journal of the Chemical Society of Japan: chemistry and industrial chemistry, 2022 January issue)
Excellent Presentation Award, Undergraduate thesis presentation, Chemistry Course, College of Science, Ibaraki University, March 2017.
Academic Excellence Award, Chemistry Course, College of Science, Ibaraki University, March 2016.
Academic Excellence Award, Chemistry Course, College of Science, Ibaraki University, March 2015.
RESEARCH SKILLS
Organic chemical synthesis
General liquid-phase synthesis method, scaling up, and process design for a wide range of molecules.
Purification with silica gel column chromatography, preparative thin-layer chromatography (PTLC), gel permeation chromatography (GPC), high performance liquid chromatography (HPLC), recrystallization, distillation.
Identification and characterization of isolated compounds with nuclear magnetic resonance (NMR), electrospray ionization high resolution mass spectrometry (ESI-HRMS), fluorescence and absorbance spectroscopy.
General solid-phase synthesis method for oligo peptide and peptoid.
In vitro experiments
Confocal laser scanning microscopy (CLSM)
Flow cytometry (FCM)
Spheroid culture
Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)
Cytotoxicity assay
Cell membrane permeability assay
Enzyme assay
DNA/RNA/protein extraction
Polymerase chain reaction (PCR)
Plasmid construction and transfection
Animal experiments
Intravenous injection
Frozen tissue sectioning
Perfusion fixation
Bio-distribution analysis
Model mice preparation (tumor-bearing, cancer metastasis, hypoxemia, hyperoxemia, inflammation, ischemia by vessels ligation)
In vivo imaging system (IVIS) fluorescence imaging
Tissue clearing
Whole-body/whole-organ clearing
Whole-body/whole-organ gel embedding
3D single-cell quantitative analysis with Imaris software