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Researchers can now explore functional connectome data from thousands of subjects made public through releases by the International Neuroimaging Data-Sharing Initiative (INDI) and 1000 Functional Connectomes Project. With this in mind, C-PAC has been designed to reliably preprocess and analyize data for hundreds of subjects in a single run, either on a single machine or on a compute cluster using Sun Grid Engine, HTCondor, or Portable Batch System.


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Different analysis pipelines can produce significantly different results, raising questions about the replicability and reliability of brain imaging findings. C-PAC makes it easy to explore the impact of particular processing decisions by allowing users to run a factorial number of analysis pipelines, each with a different set of preprocessing and analysis options. An integrated Quality Control interface facillitates rapid manual examination of pipeline outputs and selection of subjects to be included in group comparisons.

in the case of using a 1px by 1px transparent png or gif as a spacer, defining the dimensions via width, height, or style attributes will work as expected in the majority of clients, but not windows MSO (of course).

To support the entire organization, PACS must function as a comprehensive enterprise imaging system. Growing enterprises need a solution that is conducive to product growth and interoperability, extends well beyond the capabilities of a traditional PACS and unifies enterprise imaging data to support more-informed decision making. Synapse PACS is that solution.

Providing first-rate care to the millions of Americans and their families who have or continue to serve in the armed forces is imperative. To achieve this goal, healthcare providers need a PACS that unifies diverse and disparate imaging data through a single diagnostic viewer and offers extensive integration capabilities to connect the complete patient picture. Synapse PACS is that solution.

Open, server-side technology powers an advanced rules engine that brings your preferred Fujifilm and 3rd party imaging algorithms directly within the Synapse PACS workflow. Experience prioritization of studies based on findings, automated notifications to care teams on critical results, optimized workflows through automated report population, and so much more.

Synapse Analytics features a cloud-based data mining extraction engine that unifies business intelligence and Synapse PACS imaging informatics through one central point. This secure and customizable technology provides current and predictive views, tracking trends in procedure volume, patient population analysis, lab operation efficiency, profitability, diagnostic accuracy, and more.

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Another limitation is I have been assigned a windows machine. Would you recommend running fmriprep on a docker container within windows, or perhaps running it in an ubuntu environment through virtual box / wsl ?

To overcome these limitations we have developed the RootScope which generates the quantitative data required to identify new and characterize existing components of the HSR thermostat. The RootScope consists of a robust HSR reporter and an automated imaging system for monitoring the reporter in multiple plants with high temporal and spatial resolution.

To monitor the Hsp17.6p:GFP reporter we built an automated fluorescence microscopy system controlled by Manager software [34]. The microscope has a temperature-controlled chamber consisting of a Peltier heating/cooling device that accepts a petri dish with the same size format as a 96 well plate. Because condensation on the lid of the petri dish would prevent imaging we incorporated a heated glass lid into the chamber (Figure 2A-F). The chamber was mounted on a motorized XY stage to allow multiple roots to be imaged in rapid succession. The chamber can be used horizontally on the stage of a conventional upright fluorescence microscope (Figure 2G). In this orientation roots grow horizontally across the surface of the media. The chamber can also be mounted vertically on a custom fluorescence microscope (Figure 2H-I), which allows the roots to grow vertically on the surface of the media. Vertical growth is advantageous because, when grown horizontally, about half of the roots stop growing horizontally, attempt to grow into the media, and thus cannot be imaged.

The advantage of this setup compared to a microfluidic device is that we can easily and quickly plate 100 (or more) seeds on MS media on a single, cheap, and commercially available microplate. Seed placement is not critical as the roots do not have to grow in defined micro-channels. Root positions are identified manually using the Manager slide explorer plugin once the plate has been transferred to the microscope for imaging. The seeds are germinated in an incubator, grown for 3 or 4 days, and then transferred to the microscope where they can be simultaneously heat shocked and imaged. The system allows us to quantitate the HSR at high temporal (every 4 minutes with 100 plants) and spatial (~2.4m with our current camera and optics) resolution with a 12 bit dynamic range.

To characterize the attenuation of Hsp17.6 expression after a short heat shock we heat shocked Hsp17.6p:GFP for two hours and then reduced the temperature to 22C, imaging the plants every 4 minutes. This HS regimen results in the induction of Hsp17.6p:GFP at approximately one hour (starting half way through the heat shock) and attenuation at five hours. We performed this analysis with plants both homozygous and heterozygous for Hsp17.6p:GFP to determine if the dosage of the reporter affected the intensity or the kinetics of the reporter. Although the induction kinetics for heterozygotes and homozygotes were similar, plants containing a single copy of the reporter were on average only 62% as bright as plants homozygous for the reporter and attenuation occurred earlier in heterozygotes (Figure 4). This result highlights the importance of ensuring matched copy numbers when using the Hsp17.6p:GFP reporter to characterize the HSR.

The induction kinetics of Hsp17.6 in this system is slower than previous reports of HSP protein and transcript accumulation in which HSPs accumulate within half an hour of a heat shock [44, 45]. The sensitivity of our system and the maturation kinetics of GFP are both likely to contribute to this difference. Both RT-PCR and enzymatic Western blot detection techniques amplify small signals significantly making it possible to detect small numbers of molecules while our imaging approach does not. This limits the sensitivity of our system at very low Hsp17.6 expression levels. The folding, chromophore maturation, and degradation dynamics of GFP are also likely to influence the kinetics reported here, most likely by introducing delays in both the appearance and attenuation of the GFP signal. Hsp17.6p:GFP was built using pFAST-R07 which contains EGFP [39, 46]. EGFP is a GFP variant which exhibits approximately a four fold increase in the rate of chromophore maturation compared to wild type GFP. GFP variants with even faster maturation rates such as GFPmut2 or GFPm have been demonstrated to mature over twice as fast as EGFP in vitro[47] although no in planta maturation rate data exist in the literature. While the use of a rapidly maturing GFP variant has the potential to decrease the delay between transcription and detection of the GFP signal using the RootScope, there is no reporter that we are aware of which can completely eliminate this delay. Since we are not concerned with the absolute timing of these events and given the relatively rapid expression dynamics we have observed, we do not anticipate that these artifacts will prohibit the use of this system for identifying HSR thermostat mutants. In addition to using a rapidly maturing GFP to decrease delays in the onset of the signal, the use of a translational as opposed to a transcriptional reporter might result in more accurate degradation kinetics.

CPAC brings together the talents of the Black photographers who take varied journeys in their exploration of identity and history. Alanna Airitam creates and shoots scenes that undermine the misrepresentation and omission of people of color in historical narratives. Narkita Gold bring examples of her mass portrait project showing off the individuality of Black people in Denver. Rashod Taylor looks at contemporary life through the lens of family connections. 1070 Bannock St. Info: 303-837-1341 or cpacphoto.org 2351a5e196

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