CMU 2-Photon Imaging Core

Features :

• Dual scan path ( resonance-galvo for fast scanning, galvo-galvo for large field-of-view )
• Time-delay for sum frequency generation experiment ( simultaneous excitation of three fluorophores )
• Large working space for small animal imaging
• On-stage tissue incubator with CO2 and moisture control for time-lapse imaging

Detailed System Specs :

• Laser lines
  • Chameleon Discovery : dual output ( 1040 nm fixed and 680-1300 nm tunable ) combined through time-delay
  • Chameleon Vision II
• ThorLabs Bergamo II multiphoton microscope system
  • Dual scan path : Resonance-galvo scanner, galvo-galvo scanner
  • Motorized X, Y, Z
  • Fast Pockels cells for individual laser lines
  • Time-delay for spatio-temporal alignment of the two Discovery outputs
  • Forward and backward signal collection path
  • GaAsP photomultiplier tubes
  • Olympus 20X , Nikon 16X water immersion objective
• Tokai-Hit on-stage incubator
  • Temperature control
  • Moisture control
  • CO2 control ( via external supply of pre-mixed 5% CO2 )
  • Glass bottom
  • Fits culture dish <= 6cm

Applications :

• Fluorescence imaging
• Ratiometric Forster resonance energy transfer (FRET) imaging
• Wavelength mixing ( simultaneous excitation of three fluorophores at optimal wavelengths, e.g. Brainbow ) imaging
• Second harmonic generation imaging
• Time-lapse imaging
• Photobleaching, photoactivation, photoablation