Three distinct epitopes for vaccine design were predicted from the sequence of S protein. The potential B-cell epitope was KNHTSPDVDLG possessing the highest antigenicity score of 1.4039 among other B-cell epitopes. T-cell epitope for human MHC class I was VVVLSFELL with an antigenicity score of 1.0909 and binding ability to 29 MHC-I alleles. The predicted T-cell epitope for human MHC class II molecule was VVIGIVNNT with a corresponding 1.3063 antigenicity score, less digesting enzymes, and 7 MHC-II alleles binding ability. All these three peptides were predicted to be highly antigenic, non-allergenic, and non-toxic. Analyses of the physiochemical properties of these predicted epitopes indicate their stable nature for plausible vaccine design. Furthermore, molecular docking investigation between the MHC class-I epitopes and human HLA-B7 reflects the stable interaction with high affinity among them. 

Here, we have implemented a computational approach to predict potential short interfering RNAs including small interfering RNAs (siRNAs) and microRNAs (miRNAs), which are presumed to be intrinsically active against SARS-CoV-2. In doing so, we have screened the miRNA library and siRNA library targeting the ORF1ab gene. We predicted the potential miRNA and siRNA candidate molecules utilizing an array of bioinformatics tools. By extending the analysis, out of 24 potential pre-miRNA hairpins and 131 siRNAs, 12 human miRNA and 10 siRNA molecules were sorted as potential therapeutic agents against SARS-CoV-2 based on their GC content, melting temperature (Tm), heat capacity (Cp), hybridization and minimal free energy (MFE) of hybridization. This computational study is focused on lessening the extensive time and labor needed in conventional trial and error-based wet lab methods and it has the potential to act as a decent base for future researchers to develop a successful RNAi therapeutic. 

Herein, we have reviewed the various electrochemical approaches for fabricating various types of metallic mesoporous alloy films (a wide range of geometries, pore sizes, form and tunneling structures, and metal compositions). With an emphasis on the fabrication of mesoporous bimetallic and trimetallic alloy films, we discussed their specific application for developing advanced technology spanning from hydrogen production to fuel cell and sensor development. This review chronicles the obstacles and necessities implicated in the fabrication of mesoporous alloy films and provides a future perspective on the techniques offered after a full discussion in each area. We envisage that this literature will guide the researcher toward research exploring engineered alloying of earth-abundant elements and translating them into industrial-scale electrocatalysis and nanomedicine application.

In this project, an exclusive method for extracting the α-keratin from human hair waste, the Shindai extraction method, is used to make an efficient yield of 14.32% pure α-keratin. Centrifugation and dialysis against distilled water are used in order to purify the α-keratin. The UV-Vis spectroscopy is performed as an analytical indication of the presence of α-keratin in the extract. In addition, the assurance of α-keratin extraction is measured with two characteristic sharp bands in the SDS-PAGE analysis for both type-I and type-II α-keratin. This study demonstrates a viable α-keratin extraction procedure in the context of Bangladesh that has never been done before. It is important to note that the imported keratin value for Bangladesh is 895,805,141 BDT for the fiscal year 2019-2020. This rising demand for keratin must be met with its efficient industrial production of which extraction is a crucial part. Furthermore, as the consumer market for keratin continues to grow, using human hair waste as a source of α-keratin implies a cost-effective yet environmentally advantageous method that incorporates both human hair waste recycling and highly biocompatible α-keratin production, particularly for mammals.

This presentation was given by me for the Comprehensive Studies of Advanced Literature course in the Master's 1st semester. Of my enthusiastic curiosity about cancer biology and immunotherapy, I personally chose this topic that I came across approximately two years ago through the Nature Video YouTube channel. 

I picked six advanced original research articles from different publishers that comprised three cancer vaccine strategies, which showed a matter of hope in the future regarding personalized cancer treatment. To find possible similarities between these strategies, I analyzed their methodology and results in a comparative manner.