Essential oils have many antibacterial and antifungal properties. They are commonly used as a home remedy for various things like Pediculus humanus capitis infestations on humans. Essential oils are also used for their antibacterial properties and can be used to treat acne and minor abrasions. Biofilm is a thin, film like substance that is formed by bacteria and is a cause of antibiotic resistance. Biofilm is formed when bacteria or fungi excrete gelatinous exopolymers that go on to form a three dimensional polymer matrix. The biofilm is extremely hard to eradicate and is very dangerous especially in hospital settings where it can adhere to medical equipment, such as catheters. Studies have shown that essential oils may be able to disrupt the formation of biofilm, providing a less expensive way to protect medical equipment and regular household items. In the study I performed, Staphylococcus epidermidis was exposed to Melaleuca Alternifolia essential oil, or tea tree oil, before and after biofilm was formed. This will test if the essential oil can inhibit biofilm growth or disrupt the structure of the biofilm once it is already formed. Staphylococcus epidermidis was chosen as the bacteria to be tested, along with Melaleuca Alternifolia essential oil. The bacteria was cultured and then incubated on congo red agar, which had the essential oil previously placed on it. When incubated plainly with the congo red agar, the biofilm appears as a black shadow or mass in the agar. If the black shadow was significantly reduced, or completely gone, the essential oil was successful in disturbing the biofilm formation. To gather my data, I measured the intensity of the biofilm on a scale that goes up to three: 0 - no biofilm, 1 - light moderate biofilm, 2 - strong moderate biofilm, and 3 - strong biofilm. In addition, I measured the field size of the biofilm at forty-eight and ninety-six hours as well as the field size of the biofilm when essential oil was placed after biofilm was already present. I hypothesized that the Melaleuca Alternifolia essential oil would effectively prevent or disrupt Staphylococcus epidermidis biofilm formation. The results found that the essential oil was capable of preventing the growth of the bacteria and, therefore, the biofilm. However, the essential oil was not able to disrupt the structure of the previously formed biofilm. Instead, the biofilm growth was halted which shows that the essential oil can inhibit the biofilm from worsening. Through ANOVA statistical analysis test, I saw that the difference between the control of biofilm and the biofilm grown simultaneously with essential was statistically significant. Also through ANOVA, I saw that the essential oil placed after biofilm growth did have a statistical difference compared to its control. The data analysis shows the Melaleuca Alternifolia essential oil does have the ability to prevent biofilm growth and the ability to inhibit the biofilm from worsening, but does not possess the ability to disrupt the structural integrity of the biofilm polymer matrix.