In this experiment I am creating a more natural form of hormone estrogen replacement therapy (HRT) using phytoestrogen. I am going to use the phytoestrogen genistin from soy beans, since there have been various HRT studies done with the phytoestrogen red clover. I am hoping that finding a more natural hormone therapy can help girls with Turner's Syndrome and other conditions with hormone deficiencies have a better treatment than the synthetic pills and patches that they use today. Turner's Syndrome is a genetic disorder that only occurs in females and causes their growth to be stunted and some women to develop slower and have hormone deficiencies (some women with Turner's Syndrome need to even have their ovaries removed), because they only have one X chromosome. Due to these symptoms many women with Turner's Syndrome go through Hormone Replacement Therapy to go through the natural development process.

I will have two experimental groups for both the wild and Fragile-X mutant Drosophila Melanogaster in addition to control groups where the Drosophila melanogaster are not under any treatment. The two treatments are the genistin and estradiol (estrogen used in the pills and patches prescribed today) treatments. There will be twenty Drosophila melanogaster in each culture with each experimental culture having 2.88*10^-4 mg in the 20 microliters of genistin or estradiol I will put with the 14 mL I use to make my food. I used 2.88*10^-4, because it is equivalent to the dose of estradiol prescribed for women, 0.0375 mg for the later stages of the hormone replacement therapy for the body mass of a Drosophila. Furthermore, the mutation of the FMR1 gene (Fragile- X Mental retardation 1 gene) in the Fragile-X Drosophila melanogaster causes estrogen levels to be lower in females with Fragile-X, so they are being used to replicate the low estrogen levels of women with hormone deficiencies such as Turner's Syndrome.

My data suggests that the estradiol increased the reproductive rate of the Fragile-X Drosophila melanogaster, the average number of offspring per female with estradiol was 37 and for my control the average was 13.8 offspring per female. Due to the increase in the reproductive rate of the mutant Drosophila I know that my model works. The lesser impact on the wild type Drosophila melanogaster is expected, for when women with regular hormone levels use more hormone they are more at risk of cancer and the hormone has a adverse effect on their health.