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Bile salts
Sodium taurocholate
Draw 30ml of 0.9% sodium chloride solution into the 50ml syringe provided, and inject into 5.6g sodium taurocholate vial
Shake well until powder has dissolved. Invert vial and draw solution back into 50ml syringe
Connect 50ml syringe to microbial filter, filter adapter and 30ml syringe (provided)
Push solution through filter and into 30ml syringe
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