A Promising Therapeutic Strategy for Cancer Treatment: Developing Inhibitors Ta rgeting Nucleic Acid Binding Proteins Involved in Immune System Regulation and DNA Repair.

Chia Yun Wu (武家昀) 1 2 , Kuan Wei Huang (黃冠偉) 1,2 , Ya Yun Yang (楊雅韻) 1,2, Yu Yuan Hsiao (蕭育源) 1,2,

1Department of Biological Science and Technology , National Yang Ming Chiao Tung University

2Institute of Molecular Medicine and Bioengineering , National Yang Ming Chiao Tung University

The immune system is critical in protecting hosts against pathogenic microbes, viruses, and tumor growth. The activation of the human immune system through cancer immunotherapy offers a practical approa ch to treating cancer. Despite the essential role of DNA repair in maintaining genetic stability, it may also contribute to cancer cell resistance to DNA damage targeting anti cancer therapies. Targeting proteins involved in DNA repair has thus emerged as a promising therapeutic approach for cancer treatment. However, an in depth study of nucleic acid binding proteins involved in both processes is necessary to improve the fundamental understanding of immune system regulation and DNA repair and advance biome dical applications. The present study investigates cellular functions and medicinal applications of nucleic acid binding proteins related to immune regulation and DNA repair1. Structural and biochemical approaches are employed to examine the behavior of target proteins, identify potential inhibitors using structure based design, and evaluate them using inhibitor coupled activity assays. Biochemical assays, biophysics tools, and structure determination of protein inhibitor complexes are used to understand their inhibition mechanisms. The present study offers unprecedented structure to function insights, paving the way for drug design and biomedical applications of these proteins.