Adaptive laboratory evolution of Bacillus safensis as a microbial fertilizer for phenylalanine overproduction

Jade H. Su, Ethan I. Lan

Department of  molecular medicine and bioengineering , College of Biological Science and Technology

National Yang Ming Chiao Tung University, Hsinchu City, Taiwan  

Currently, diseases of affluence such as hypertension, Diabetes, obesity, and cancer raise great attention in the health care industry. Curcumin, an aromatic compound produced by Curcuma longa, is derived from phenylalanine and has an important role as a health care product as it has numerous pharmacological effects such as lowering blood triacylglycerides, anti-cancer, and preventing prediabetes. To overproduce induce higher curcumin biosynthesis by Curcuma longa, here we engineered a rhizobacteria - Bacillus safensis as a microbial fertilizer for optimized soil fertility to promote plant growth and overexpressed aromatic amino acid biosynthetic pathway for providing phenylalanine as a precursor to curcuminoid biosynthesis.