Update: SPLiT-seq kits are available through Split Bio, a company founded by the authors of SPLiT-seq. The kits offer vastly improved sensitivity (UMIs and genes per cell) and dramatically simplify the workflow. For more information please visit: www.splitbio.com
Split Pool Ligation-based Transcriptome sequencing (SPLiT-seq) is a single cell RNA sequencing method developed in the Seelig Lab at the University of Washington.
We hope this website will facilitate the adoption of SPLiT-seq in labs interested in applying single cell RNA sequencing to their work. The current protocol along with other resources to get you started can be found under the "protocol" tab.
See our paper in Science here.
"Single-cell profiling of the developing mouse brain and spinal cord with split-pool barcoding "
Alexander B. Rosenberg*, Charles M. Roco*, Richard A. Muscat , Anna Kuchina , Paul Sample, Zizhen Yao, Lucas Gray, David J. Peeler, Sumit Mukherjee , Wei Chen , Suzie H. Pun, Drew L. Sellers, Bosiljka Tasic, Georg Seelig.
How does it work?