• Upon entering Flow Facility Lab, all researchers are to put on their lab coats, gloves and covered shoes.

• No food or drinks are allowed in the facility.

• Samples :

Please identify samples as below:

1. Cell lines / blood samples / isolated primary cells including stem cells / microbes;

2. Potentially carry bloodborne pathogens such as HIV, SIV, HPV, HCV, HSV, HTLV-1, HTLV-2, CMV;

3. Potentially carry airborne pathogens such as SARS, Avian Influenza A, Mycobacterium tuberculosis, Mycobacterium bovis, Neisseria meningitidis, Mycoplasma.

4. Treated with live isolates of 1. and 2.

5. Radioactive or labelled with radioactive reagents.

Samples from 3 -5 are not allowed into the lab and not allowed to run on the machines!

• Sample Preparation
  1. Researchers must plan out their experiments before booking of equipment.

2. Experiments that uses two or more fluorescents are to prepare compensation beads or cells (inclusive of an unstained tube).
    3. The recommended concentration of cells 1x10⁶million cells/ml.
    4. It is important that researchers must filter their samples before coming down to acquire.

• Permission Usage

     Please complete the “Request and Booking Form for Lab Service and Equipment” at the below and forward the completed form to the lab staff before booking        a Flow Cytometry session.

• Booking
  Researchers are only allowed to book two weeks in advance and cancel three days in advance.  For any enquiries on flow cytometry, feel free to email the flow facility staffs at medic.putralmas@upm.edu.my