What you can learn in this lab?

The major topic for my research is about the drug delivery system. Drug delivery systems are defined as formulations or engineered methods that introduce pharmaceutical compounds to accomplish therapeutic effects in the body. In my lab, we design peptide-base vesicles and nanoparticles for not only therapeutic molecules delivery but also specific gene for gene therapy. Here, you will learn various experimental skills, such as plasmid construction and gene recombination, cell culture and pharmaceutical treatment concepts, flow cytometry, fluorescent confocal microscopy, and TEM/SEM microscopy. We sincerely welcome students who are interesting in these researches.

Publications

a). Scientific journals with impact factor

1. Liu BR, Huang YW, Korivi M, Lo SY, Aronstam RS, Lee HJ (2017). The Primary Mechanism of Cellular Internalization for a Short Cell- Penetrating Peptide as a Nano-Scale Delivery System. Current Pharmaceutical Biotechnology, 18(7):569-584.

2. Liu BR, Huang YW, Aronstam RS, Lee HJ (2016). Identification of a Short Cell-Penetrating Peptide from Bovine Lactoferricin for Intracellular Delivery of DNA in Human A549 Cells. PLoS One, 11(3):e0150439.

3. Liu BR, Huang YW, Aronstam RS, Lee HJ (2015). Comparative Mechanisms of Protein Transduction Mediated by Cell-Penetrating Peptides in Prokaryotes. Journal of Membrane Biology, 248(2):355-368.

4. Liu BR, Chen HH, Chan MH, Huang YW, Aronstam RS, Lee HJ (2015). Three Arginine-Rich Cell-Penetrating Peptides Facilitate Cellular Internalization of Red-Emitting Quantum Dots. Journal of Nanoscience and Nanotechnology, 15(3):2067-2078.

5. Liu BR, Liou JS, Chen YJ, Huang YW, Lee HJ (2013). Delivery of nucleic acids, proteins, and nanoparticles by arginine-rich cell-penetrating peptides in rotifers. Marine Biotechnology, 15(5):584-95.

6. Liu BR, Lo SY, Liu CC, Chyan CL, Huang YW, Aronstam RS, Lee HJ (2013). Endocytic trafficking of nanoparticles delivered by cell-penetrating peptides comprised of nano-arginine and a penetration accelerating sequence. PLoS One, 8(6):e67100.

7. Liu BR, Winiarz JG, Moon JS, Lo SY, Huang YW, Aronstam RS, Lee HJ (2013). Synthesis, characterization and applications of carboxylated and polyethylene-glycolated bifunctionalized InP/ZnS quantum dots in cellular internalization mediated by cell-penetrating peptides. Colloids and Surfaces B: Biointerfaces, 111C:162-170.

8. Liu BR, Liou JS, Huang YW, Aronstam RS, Lee HJ (2013). Intracellular delivery of nanoparticles and DNAs by IR9 cell-penetrating peptides. PLoS One, 8:e64205.

9. Liu BR, Huang YW, Lee HJ (2013). Mechanistic studies of intracellular delivery of proteins by cell-penetrating peptides in cyanobacteria. BMC Microbiology, 13, 57.

10. Liu BR, Chiang HJ, Huang YW, Chan MH, Chen HH, Lee HJ (2013).Cellular internalization of quantum dot mediated by cell-penetrating peptides. Pharmaceutical Nanotechnology, 1, 151-161.

11. Huang YW, Lee HJ, Liu BR, Chiang HJ, Wu CH (2013). Cellular internalization of quantum dots. Methods in Molecular Biology, 991, 249-259.

12. Liu BR, Huang YW, Chiang HJ, Lee HJ (2013). Primary effectors in the mechanisms of transmembrane delivery of arginine-rich cell-penetrating peptides. Advanced Studies in Biology, 5(1), 11-25.

13. Liou JS, Liu BR, Martin AL, Huang YW, Chiang HJ, Lee HJ (2012).Protein transduction in human cells is enhanced by cell-penetrating peptides fused with an endosomolytic HA2 sequence. Peptides, 37(2), 273–284.

14. Liu BR, Lin MD, Chiang HJ, Lee HJ (2012). Arginine-rich cell-penetrating peptides deliver gene into living human cells. Gene, 505, 37-45.

15. Chen YJ, Liu BR, Dai YH, Lee CY, Chan MH, Chen HH, Chiang HJ, Lee HJ (2012). A gene delivery system for insect cells mediated by arginine-rich cell-penetrating peptides. Gene, 493, 201-210.

16. Dai YH, Liu BR, Chiang HJ, Lee HJ (2011). Gene transport and expression by arginine-rich cell-penetrating peptides in Paramecium. Gene, 489, 89-97.

17. Liu BR, Huang YW, Winiarz JG, Chiang HJ, Lee HJ (2011). Intracellular delivery of quantum dots mediated by a histidine- and arginine-rich HR9 cell penetrating peptide through the direct membrane translocation mechanism. Biomaterials, 32, 3520-3537.

18. Liu BR, Huang YW, Chiang HJ, Lee HJ (2010). Cell-penetrating peptide functionized quantum dots for intracellular delivery. Journal of Nanoscience and Nanotechnology, 10, 7897-7905.

19. Liu BR, Li JF, Lu SW, Lee HJ, Huang YW, Shannon KB, Aronstam RS (2010). Cellular internalization of quantum dots noncovalently conjugated with arginine-rich cell-penetrating peptides. Journal of Nanoscience and Nanotechnology, 10, 6534-6543.

20. Xu Y, Liu BR, Lee HJ, Shannon KS, Winiarz JG, Wang TC, Chiang HJ, Huang YW (2010). Nona-arginine facilitates delivery of quantum dots into cells via multiple pathways. Journal of Biomedicine and Biotechnology, 2010, 948543.

21. Lu SW, Hu JW, Liu BR, Lee CY, Li JF, Chou JC, Lee HJ (2010). Arginine rich intracellular delivery peptides synchronously deliver covalently and noncovalently linked proteins into plant cells. Journal of Agricultural and Food Chemistry, 58, 2288-2294.

22. Hu JW, Liu BR, Wu CY, Lu SW, Lee HJ (2009). Protein transport in human cells mediated by covalently and noncovalently conjugated arginine-rich intracellular delivery peptides. Peptides, 30, 1669-1678.

23. Liu BR, Chou JC, Lee HJ. (2008). Cell membrane diversity in noncovalent protein transduction. Journal of Membrane Biology, 222, 1-15.

24. Hou YW, Chan MH, Hsu HR, Liu BR, Chen CP, Chen HH, Lee HJ. (2007). Transdermal delivery of proteins mediated by non-covalently associated arginine-rich intracellular delivery peptides. Experimental Dermatology, 16, 999-1006.

25. Chen CP, Chou JC, Liu BR, Chang M, Lee HJ. (2007). Transfection and expression of plasmid DNA in plant cells by an arginine-rich intracellular delivery peptide without protoplast preparation. FEBS Letters, 581, 1891-1897.

26. Chang M, Chou JC, Chen CP, Liu BR, Lee HJ. (2007). Noncovalent protein transduction in plant cells by macropinocytosis. New Phytologist, 174, 46-56.

b). Book chapters

1. Liu BR, Huang YW, and Lee HJ. (2019). Hypotoxic Fluorescent Nanoparticles Delivery by Cell-Penetrating Peptides in Multiple Organisms: From Prokaryotes to Mammalians Cells. In: Bioengineering. London, UK: IntechOpen Limited Inc. DOI: 10.5772/intechopen.83818

2. Liu BR, Huang YW, Aronstam RS, and Lee HJ. (2014). Chapter 8 Internalization of noncovalently associated proteins by cell-penetrating peptides in cyanobacteria. In: Cyanobacteria Ecological Importance, Biotechnological Uses and Risk Management. Hauppauge, New York, USA: Nova Science Publishers Inc. pp. 171-180.

3. Liu BR, Chan MH, Chen HH, Lo SY, Huang YW, Lee HJ. (2013). Chapter 4 Protein transduction in human cells mediated by arginine-rich cell-penetrating peptides in mixed covalent and noncovalent manners. In: Macromolecular Chemistry: New Research. New York, USA: Nova Science Publishers Inc. pp. 69-82.

4. Liu BR, Chan MH, Chen HH, Lo SY, Huang YW, Lee HJ. (2013). Chapter 3 Effects of Surface Charge and Particle Size of Cell-Penetrating Peptide/Nanoparticle Complexes on Cellular Internalization. In: Cell Membrane: Molecular Structure, Physiochemical. New York, USA: Nova Science Publishers Inc. pp. 43-57 (ISBN: 978-1-62808-456-6).

5. Liu BR, Chiang HJ, Lee HJ. (2011). Chapter 10 Arginine-Rich Cell-Penetrating Peptides in Cellular Internalization. In: Arginine Amino Acid. Jacobs N.L. Ed., Nova Science Publishers Inc., Hauppauge, New York, pp. 207-218.

6. Lee CY, Liu BR, Wang YH, Hou YW, Chou JC, Lee HJ. (2011). Chapter 15 RNA Interference Mediated by Cell-Penetrating Peptides. In: Gene Silencing: Theory, Techniques and Applications. Catalano A.J. Ed., Nova Science Publishers Inc., Hauppauge, New York, pp. 335-342.

7. Liu BR, Chen CY, Chen RL, Chiang HJ, Huang YW, Lee HJ. (2010). Chapter 8 Macropinocytosis: possible mechanisms of cellular entry of arginine-rich intracellular delivery peptides. In: Endocytosis: Structural Components, Functions and Pathways. Dowler B.C. Ed., Nova Science Publishers Inc., Hauppauge, New York, pp. 176-190.

8. Liu BR, Hou YW, Chan MH, Chen HH, Lee HJ. (2010). Chapter 15 A novel transdermal drug delivery system mediated by arginine-rich intracellular delivery peptides. In: Dermatology Research Focus on Acne, Melanoma and Psoriasis. Roth D.E. Ed., Nova Science Publishers Inc., Hauppauge, New York, pp. 281-292.

9. Lee HJ, Chou JC, Chen CP, Liu BR. (2008). DNA transfection and expression mediated by arginine-rich intracellular delivery peptides in plant cells. In: Transgenic Plants: New Research. Chan O.T. Ed., Nova Science Publishers Inc., Hauppauge, New York, pp. xix-xxvi.

c). Symposium

1. Huang YW, Liu BR, Lee HJ (2012, Nov). Routes of cellular uptake of nanosized materials depend on compositions of cell-penetrating peptides. The 1st Annual Meeting of Sustainable Nanotechnology Organization, VA, USA.

2. Liu BR, Lee HJ, Huang YW, Aronstam RS (2017, July) Mechanistic Studies of Intracellular Delivery of Molecules by Cell Penetrating Peptides in Human Lung Carcinoma A549 Cells. BIT's 15th Annual Congress of IDDST, OSA, Japan.

3. Singh B, Tsao LI, Soo PC, Lai MJ, Liu BR (2018, March) Effects of Copper Nanoparticle on Escherichia coli. The 33th Joint Annual Conference of Biomedical Science, Taipei, Taiwan.