3D printing has become a useful method of curating hydrogels since it allows for the uniform distribution of cells throughout the geometry of the scaffold. Agarose has been the common medium for creating these 3D-printed hydrogels in the past; however, this polymer causes issues with cellular attachment and proliferation. Therefore, I have created a 3D printer capable of producing alginate-based, ionically crosslinked hydrogels. These hydrogels are mechanically comparable to those produced in traditional fabrication methods, are capable of being fabricated in diameters consistent with the native extracellular matrix, and have allowed for successful cellular integration into the printing system. The development of this system will be useful in fabricating tailorable scaffolds for the regeneration of various tissues.

Danielle would like to thank her mentor, Dr. Scott Sell, for the opportunity to work and learn in his laboratory throughout her undergraduate education. Danielle would also like to thank Dr. Robert Pampel and the Honor’s College staff for all of their support as well as the funding from the University Honors Program that was used to support this project through the Investigative Learning Experience (ILEX) grant.