Reactive oxygen species (ROS) have proven invaluable in extracting vital molecular information in various bioanalytical techniques. However, we are particularly interested in investigating the potential of atomic oxygen, O(3P), in these methods because compared to other ROS, O(3P) has a higher selectivity for electrophilic oxidation of alkenes and thiols. Unfortunately, previous research on cellular responses to O(3P) has been limited due to the harsh conditions required to generate O(3P) and the associated by-products. To address these challenges, we have synthesized 2,8-diaxetoxymethylDBTO, a water-soluble molecule that produces O(3P) upon UV-A irradiation through photodeoxygenation. With this clean source of O(3P), we can now study the effects of O(3P) on cell viability, gene expression, and cell cycle distribution.

Bashar would like extend his gratitude to his faculty sponsor, Dr. McCulla, for all the invaluable guidance and support provided throughout the past few years. With patience and expertise, Dr. McCulla has illuminated the intricacies of the difficult world of organic synthesis, inspiring Bashar to hone his critical thinking skills, cultivate curiosity, and persevere through challenges.