Ved P. Sharma, PhD
Research Assistant Professor
Albert Einstein College of Medicine
Department of Anatomy and Structural Biology
Bronx, New York
Email: ved.sharma (at) einstein (dot) yu (dot) edu

Research key words
Tumor microenvironment, Breast cancer biology, Cancer stem cells, Invadopodia regulation, in vivo cell migration and invasion, Phospholipid signaling

Molecular biology, Live-cell fluorescence microscopy, Multidimensional image analysis using ImageJ, Statistical analysis using R

  1. Karagiannis GS, Pastoriza JM, Wang Y, Harney AS, Entenberg D, Pignatelli J, Sharma VP, Xue EA, Cheng E, D'Alfonso TM, Jones JG, Anampa J, Rohan TE, Sparano JA, Condeelis JS, Oktay MH. Neoadjuvant chemotherapy induces breast cancer metastasis through a TMEM-mediated mechanism. Sci Transl Med. 2017 Jul 5;9(397)
  2. Eddy RJ, Weidmann MD, Sharma VP, Condeelis JS. Tumor Cell Invadopodia: Invasive Protrusions that Orchestrate Metastasis. Trends Cell Biol. 2017 Apr 12. doi: 10.1016/j.tcb.2017.03.003.
  3. Leung E, Xue A, Wang Y, Rougerie P, Sharma VP, Eddy R, Cox D, Condeelis J. Blood vessel endothelium-directed tumor cell streaming in breast tumors requires the HGF/C-Met signaling pathway. Oncogene. 2016 Nov 28. doi: 10.1038/onc.2016.421.
  4. Weidmann MD, Surve CR, Eddy RJ, Chen X, Gertler FB, Sharma VP, Condeelis JS. MenaINV dysregulates cortactin phosphorylation to promote invadopodium maturation. Scientific Reports. 2016 Nov 8;6:36142. doi: 10.1038/srep36142.
  5. Carmona G, Perera U, Gillett C, Naba A, Law AL, Sharma VP, Wang J, Wyckoff J, Balsamo M, Mosis F, De Piano M, Monypenny J, Woodman N, McConnell RE, Mouneimne G, Van Hemelrijck M, Cao Y, Condeelis J, Hynes RO, Gertler FB, Krause M. Lamellipodin promotes invasive 3D cancer cell migration via regulated interactions with Ena/VASP and SCAR/WAVE. Oncogene. 2016 Mar 21. doi: 10.1038/onc.2016.47
  6. Jimenez L, Sharma VP, Condeelis J, Harris T, Ow TJ, Prystowsky MB, Childs G, Segall JE. MicroRNA-375 Suppresses Extracellular Matrix Degradation and Invadopodial Activity in Head and Neck Squamous Cell Carcinoma. Arch Pathol Lab Med. 2015 Nov;139(11):1349-61
  7. Valenzuela-Iglesias A, Sharma VP, Beaty BT, Ding Z, Gutierrez-Millan LE, Roy P, Condeelis JS, Bravo-Cordero JJ. Profilin1 regulates invadopodium maturation in human breast cancer cells. European Journal of Cell Biology 2015 Feb;94(2):78-89
  8. Sharma VP, Beaty BT, Cox D, Condeelis JS, Eddy RJ. An In Vitro One-Dimensional Assay to Study Growth Factor-Regulated Tumor Cell–Macrophage Interaction. Methods in Molecular Biology 2014;1172:115-23
  9. Beaty BT, Wang Y, Bravo-Cordero JJ, Sharma VP, Miskolci V, Hodgson L, Condeelis J. Talin regulates moesin–NHE-1 recruitment to invadopodia and promotes mammary tumor metastasis. Journal of Cell Biology 2014 Jun 9;205(5):737-51
  10. Sharma VP, Eddy R, Entenberg D, Kai M, Gertler F, Condeelis J. Tks5 and SHIP2 regulate invadopodium maturation, but not initiation, in breast carcinoma cells. Current Biology 2013 Nov 4, 23(21):2079-89
  11. Roh-Johnson M, Bravo-Cordero JJ, Patsialou A, Sharma VP, Guo P, Liu H, Hodgson L, Condeelis J. Macrophage contact induces RhoA GTPase signaling to trigger tumor cell intravasation. Oncogene 2013, doi: 10.1038/onc.2013.377 
  12. Zhou ZN, Sharma VP, Beaty BT, Roh-Johnson M, Peterson EA, Van Rooijen N, Kenny PA, Wiley HS, Condeelis JS, Segall JE. Autocrine HBEGF expression promotes breast cancer intravasation, metastasis and macrophage-independent invasion in vivo. Oncogene 2013 Sep 9, doi: 10.1038/onc.2013.363
  13. Sharma VP, Entenberg D, Condeelis J. High-resolution live-cell imaging and time-lapse microscopy of invadopodium dynamics and tracking analysis. Methods in Molecular Biology 2013; 1046:343-57
  14. Bravo-Cordero JJ, Sharma VP, Roh-Johnson M, Chen X, Eddy R, Condeelis J, Hodgson L. A GEF/GAP modulator defines protrusion polarity in migrating cells. Journal of Cell Science 2013 Aug 1;126:3356-69
  15. Beaty, BT, Sharma VP, Bravo-Cordero JJ, Simpson MA, Eddy R, Koleske AJ, Condeelis J. β1 integrin regulates Arg to promote invadopodial maturation and matrix degradation. Molecular Biology of the Cell 2013 Jun;24(11):1661-75
  16. Sharma VP, Beaty BT, Patsialou A, Liu H, Clarke M, Cox D, Condeelis JS, Eddy RJ. Reconstitution of in vivo macrophage-tumor cell pairing and streaming motility on one-dimensional micro-patterned substrates. IntraVital 2012; 1:1, 77-85.
  17. Sharma VP, DesMarais V, Sumners C, Shaw G, Narang A. Immunostaining evidence for PI(4,5)P2 localization at the leading edge of chemoattractant-stimulated HL-60 cells. Journal of Leukocyte Biology 2008; 84(2), 440-447
  18. Ahmed Z, Shaw G, Sharma, VP, Yang C, McGowan E, Dickson DW. Actin binding proteins coronin-1a and iba-1 are effective microglial markers for immunohistochemistry. Journal of Histochemistry and Cytochemistry 2007; 55(7), 687-700
  19. Ahmed Z, Shaw G, Sharma, VP, McGowan E, Dickson DW. Coronin-1a: a novel microglial marker for use in paraffin embedded tissue: 41.1. Journal of Neuropathology and Experimental Neurology 2007; 66(5), 420-420