Protocols

See the lab web page for more info on the work being done.

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Simple column purification protocol to prepare BAC constructs for pronuclear microinjection.   97k v. 2 Apr 19, 2011, 12:02 PM Todd Valerius
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These BAC minipreps yield sufficient DNA for PCR and sequencing. Good for screening clones from recombineering.  51k v. 1 Jun 9, 2009, 10:58 AM Todd Valerius
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The BAC recombineering protocol we used successfully to create several BAC constructs for microinjection into mice. This is based on the method from Copeland et al. that utilizes a temperature inducible recombination system (EL250 bacterial strain).  82k v. 1 Jun 9, 2009, 10:56 AM Todd Valerius
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A simple protocol for screening bacterial colonies by PCR directly (i.e. no minipreps).  55k v. 1 Jan 1, 2013, 8:49 AM Todd Valerius
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This protocol is designed to create hybrid homology arms for BAC recombination, but can easily be adopted for use on other cloning projects to shorten cloning steps. This protocol is based on one from JrGang Cheng at UNC.  153k v. 2 Aug 15, 2012, 10:52 AM Todd Valerius
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Sheet for scoring the health of mice after a surgical procedure. This scoring system is referenced highly in guides for developing appropriate animal use protocols. This sheet is simply my layout of the system for easy printing.  84k v. 1 May 11, 2010, 7:09 AM Todd Valerius
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Riboprobe synthesis from PCR amplifies template.  105k v. 1 Jun 9, 2009, 10:01 AM Todd Valerius
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Section in situ hybridization (SISH) protocol. This protocol is adapted from the WISH version for use on frozen kidney sections with Dig-labelled riboprobes.  145k v. 1 Jul 22, 2010, 10:52 AM Todd Valerius
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This is the solution guide for using the BioLane for the in situ washes. Includes amount needed for various plate sizes.  56k v. 1 Jun 9, 2009, 10:07 AM Todd Valerius
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Whole-Mount In Situ Hybridization Protocol. This is the protocol followed for our work on the GUDMAP.org project. It includes using the BioLane machine for the washes.  190k v. 1 Jun 9, 2009, 10:00 AM Todd Valerius
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Used for both wholemount samples and frozen sections to detect Beta-galactosidase activity.   82k v. 2 Aug 15, 2012, 10:53 AM Todd Valerius
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