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Sample Submission

  1. Prepare your templates (see Template section).
  2. Prepare your primers (see Primers section).
  3. For non pre-mixed template and primer: please submit 10µL DNA template and 10µL primer in separate tubes. Do not mix template and primer together. Sample names must be written on the tubes.
  4. For premixed reactions, please mix 5µL of DNA template at a concentration of 0.3µg - 0.1µg /µL (Plasmid DNA), 20 ng DNA per 100 base pairs product/µL (PCR product), 0.4 µg/µL (Cosmids or lambda) or 0.1 µg/µL (Single-stranded phage) with 5µL of primer at a concentration of 5 µM. For Free Primers provided by Umass Genomics Resource Lab, click here.  Minimum final volume will be 10µL in 96 well, "V" bottom plates.
  5. For Ready-to-Run Plates, please use ABI MicroAmp Optical 96-Well Reaction Plate, Cat. No. N8010560.
  6. Users must include a printed Sequencing Order Form. The Sequencing Order Form is available here. Please also email your Sequencing Order Form to genomics@research.umass.edu. Please do not use any illegal characters for your sample names,otherwise we are not responsible for wrong sample names or typographical errors.
  7. You may send or mail samples to:

> Genomics Research Lab
> University of Massachusetts
> Fernald Hall, B1
> 270 Stockbridge Road
> Amherst, MA 01003
> 413-545-1039