1) Bacterial polysaccharide biosynthesis systems are a key interest in our group with an emphasis on developing in vitro systems for the enzymatic synthesis of these complex polymers. The goal of this series of projects is to develop libraries of well-characterized enzymes for the assembly of these materials. The eventual application of this work will be to have methods to produce any bacterial polysaccharide for biomedical applications such as therapeutics based on symbiotic bacterial capsules or vaccines against pathogenic bacteria. In addition, this work will eventually focus on the biophysical properties of these complex polysaccharide materials to understand their diverse ranges of bioactivity. Work in this area typically involves techniques in molecular biology, protein expression, isolation and purification, and the use of bioanalytical techniques such as Capillary Electrophoresis and High Performance Liquid Chromatography. Typical organic characterization techniques are also utilized including Mass Spectral identification of products and multidimensional NMR techniques.
2) Bacterial polysaccharide biosynthesis probes will be and have been developed in our group for characterizing the assembly of these molecules. The goal of projects in this area is to more easily track the biosynthesis of complex polysaccharide polymers on the isoprenoid scaffold bactoprenyl phosphate. Our interest here is in developing both in vitro and in cellulo probes for these assembly pathways to identify enzymes responsible for their biosynthesis. To do this we use tools in organic chemistry, chemical biology and enzymology. The ultimate goal of this area of research is to design novel probes for characterizing these materials to identify potential targets for anti-bacterial agents.
3) Undecaprenyl Pyrophosphate Synthase (UPPS) is a critical enzyme for bacterial survival. Our goal in this series of projects is to understand how UPPS from different species recognize different substrate structural motifs. With knowledge of these differences we will begin the development of new species selective inhibitors for UPPS. The work in these projects will utilize techniques in organic chemistry, molecular biology, site-directed mutagenesis, protein expression, isolation and purification, as well as method development in rapid assay design and HPLC methodology. We have also established a strong collaboration with Dr. Donald Jacobs of the UNC-Charlotte Physics Department for the development of computational tools to study this protein.
The above image is a representative structure of a UPPS protein highlighting the key areas for substrate interactions and elongation of bactoprenyl diphosphate.
4) Developing protein-lipid hybrid building blocks for nanoscale science applications. A major focus of the UNC-Charlotte Chemistry department is in Nanoscale Science. Our work in polysaccharide biosynthesis will eventually move in that direction, but we have also recently begun developing a research program that focuses on the development of self assembling building blocks with proteins embedded in lipid-protein nanoparticles called nanodiscs (nanodiscs were first developed by the Sligar group at the University of Illinois Urbana-Champagne). The goal of projects in this area is to assemble protein specific biomachines taking advantage of protein-protein interactions well conserved in nature. The eventual applications of these materials could be in the development of nanofiltration systems, reaction centers, and metamaterials.