SparkMaster

Calcium Spark Analysis with ImageJ


 

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SparkMaster is an ImageJ plugin that allows easy and reliable calcium spark analysis in confocal line-scan images.

SparkMaster is published in the American Journal of Physiology - Cell Physiology. Click here to download the pdf version or here to see the pubmed reference of the paper.

The underlying analysis algorithm used in SparkMaster is adapted from the threshold-based standard method of spark analysis developed by Cheng et al. (Biophys J 1999; 76:606-17). SparkMaster offers a graphical user interface through which all analysis parameters and output options are selected. The analysis includes general image parameters (number of detected sparks, spark frequency) as well as individual spark parameters (Amplitude, FWHM, FDHM, Full Width, Full Duration, Time-to-Peak, maximum steepness of spark upstroke, time constant of spark decay).

Download the SparkMaster plugin here.