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Physiology

posted Mar 4, 2011, 8:12 AM by rajesh gk

Title

Effect of VAM fungi and bacterial biofertilizers on mulberry leaf quality and silkworm cocoon characters under semiarid conditions

Authors

Ram Rao, D.M*, Kodandaramaiah, J., Reddy, M.P., Katiyar, R.S., Rahmathulla, V.K.,

Journal

Caspian J. Env. Sci., 5, 111-117. ( 2007 )

Abstract

The influence of VAM fungi and bacterial biofertilizer (BBF) with 50% reduction in the recommended dose of (N and P) chemical fertilizers on leaf quality traits of mulberry variety (S-13) and its impact on silkworm (PM ? NB4D2) growth and cocoon characters were studied under semi-arid conditions. Four different treatments were imposed i.e., T1: Control (only 100% NPK); T2: VAM (50% cut in P); T3: BBF (50% cut in N) and T4: BBF and VAM (50% cut in N and P). The results revealed that reduction (50%) in the dose of chemical fertilizers in T2, T3 and T4 did not affected the leaf quality traits or cocoon parameters, this may be due to the effect of microbial inoculants in these treatments, which had efficiently regulated the normal growth, metabolism and physiological activity in plants. Among the three-biofertilizer treatments, leaf quality, silkworm growth and cocoon parameters were found improved in T4 and was on par with T1 control. The dual inoculation (T4) proved economical and beneficial with regard to saving of 50 % cost of chemical fertilizers and improvement in soil fertility, leaf quality and cocoon parameters, thus this technology can be recommended to sericulture

Citation

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Title

UV-B Induced Changes in the Secondary Metabolites of Morus alba L. Leaves

Authors

Xi-Da Gu ; Ming-Yao Sun ; Lin Zhang ; Hong-Wei Fu ; Lei Cui ; Run-Ze Chen ; Da-Wei Zhang ; Jing-Kui Tian

Journal

Molecules, 2010; 15(5) 2980-2993

Abstract

Ultraviolet-B (UV-B) radiation is harmful to plants and human beings. Many secondary metabolites, like flavonoids, alkaloids, and lignin, are UV-B absorbing compounds, which can protect the genetic material of plants. Furthermore, they are active components of herbal drugs. UV-B radiation can activate the self-protective secondary metabolism system. The results of this paper provide a method to induce bioactive secondary metabolites from mulberry leaves (Morus alba L.) by UV-B irradiation in vitro. Five significantly different chromatographic peaks were found by HPLC fingerprint after induction, from which two active compounds were identified: One was chalcomoracin, a natural Diels-Alder type adduct with antibacterial activity; the other one was moracin N, which is a precursor of chalcomoracin. Their contents were 0.818 mg/g and 0.352 mg/g by dry weight, respectively.

Citation

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posted Jul 9, 2010, 9:09 AM by rajesh gk

Title

Determination of iminosugars in mulberry leaves and silkworms using hydrophilic interaction chromatography-tandem mass spectrometry.

Authors

Nakagawa K, Ogawa K, Higuchi O, Kimura T, Miyazawa T, Hori M.

 

Food and Biodynamic Chemistry Laboratory, Graduate School of Agricultural Science, Tohoku University, Sendai 981-8555, Japan.

Journal

Anal Biochem. 2010 May 12. [Epub ahead of print]

Abstract

Mulberry 1-deoxynojirimycin (DNJ, a potent alpha-glycosidase inhibitor) has been investigated thoroughly for its analytical methods and therapeutic potential against diabetes, whereas little attention has been given to other iminosugars such as 2-O-alpha-d-galactopyranosyl-DNJ (GAL-DNJ) and fagomine. For instance, concentration and composition of these iminosugars in mulberry leaves as well as sericulture products have not been fully characterized due to lack of suitable analytical methods. Here we developed a simultaneous determination method for DNJ, GAL-DNJ, and fagomine using hydrophilic interaction chromatography (HILIC) with tandem mass spectrometry (MS/MS). When mulberry leaf extracts were subjected to HILIC-MS/MS with multiple reaction monitoring (MRM), individual iminosugars could be separated and detected. The developed method is sufficiently sensitive for determining iminosugars in mulberry leaves as well as silkworms, providing new information (e.g., different amounts of iminosugars in mulberry leaf varieties; high DNJ and low GAL-DNJ in the silkworm body, especially in the blood) that is useful for producing iminosugar-rich products for nutraceutical purposes. Copyright © 2010 Elsevier Inc. All rights reserved.

Citation

http://www.ncbi.nlm.nih.gov/pubmed/20470744 PMID: 20470744 [PubMed - as supplied by publisher]

posted Jul 4, 2010, 6:07 AM by rajesh gk

 
 

Title

Leaf cuticular wax amount and crystal morphology regulate post-harvest water loss in mulberry (Morus species).

Authors

Mamrutha HM, Mogili T, Jhansi Lakshmi K, Rama N, Kosma D, Udaya Kumar M, Jenks MA, Nataraja KN.

 

Department of Crop Physiology, University of Agricultural Sciences, GKVK, Bangalore 560 065, India.

Journal

Plant Physiol Biochem. 2010 May 9. [Epub ahead of print]

Abstract

Mulberry leaves are the sole source of food for silkworms (Bombyx mori), and moisture content of the detached leaves fed to silkworms determines silkworm growth and cocoon yield. Since leaf dehydration in commercial sericulture is a serious problem, development of new methods that minimize post-harvest water loss are greatly needed. In the present study, variability in moisture retention capacity (MRC, measured as leaf relative water content after one to 5 h of air-drying) was examined by screening 290 diverse mulberry accessions and the relationship between MRC and leaf surface (cuticular) wax amount was determined. Leaf MRC varied significantly among accessions, and was found to correlate strongly with leaf wax amount. Scanning electron microscopic analysis indicated that leaves having crystalline surface waxes of increased facet size and density were associated with high MRC accessions. Leaf MRC at 5 h after harvest was not related to other parameters such as specific leaf weight, and stomatal frequency and index. This study suggests that mulberry accessions having elevated leaf surface wax amount and crystal size and density exhibit reduced leaf post-harvest water loss, and could provide the foundation for selective breeding of improved cultivars. Copyright © 2010 Elsevier Masson SAS. All rights reserved.

Citation

PMID: 20580887 [PubMed - as supplied by publisher]

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