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Pathology

posted Mar 4, 2011, 8:09 AM by rajesh gk

Title

Shotgun proteomic analysis of mulberry dwarf phytoplasma

Authors

Ji Xianling ; Gai Yingping ; Lu Baoyun ; Zheng Chengchao ; Mu Zhimei

Journal

Proteome Science

Abstract

Background: Mulberry dwarf (MD), which is caused by phytoplasma, is one of the most serious infectious diseases of mulberry. Phytoplasmas have been associated with diseases in several hundred plant species. The inability to culture phytoplasmas in vitro has hindered their characterization at the molecular level. Though the complete genomes of two phytoplasmas have been published, little information has been obtained about the proteome of phytoplasma. Therefore, the proteomic information of phytoplasmas would be useful to elucidate the functional mechanisms of phytoplasma in many biological processes.

Results: MD phytoplasmas, which belong to the 16SrI-B subgroup based on the 16S DNA analysis, were purified from infected tissues using a combination of differential centrifugation and density gradient centrifugation. The expressed proteome of phytoplasma was surveyed by one-dimensional SDS-PAGE and nanocapillary liquid chromatography-tandem mass spectrometry. A total of 209 phytoplasma proteins were unambiguously assigned, including the proteins with the functions of amino acid biosynthesis, cell envelope, cellular processes, energy metabolism, nucleosides and nucleotide metabolism, replication, transcription, translation, transport and binding as well as the proteins with other functions. In addition to these known function proteins, 63 proteins were annotated as hypothetical or conserved hypothetical proteins.

Conclusions: Taken together, a total of 209 phytoplasma proteins have been experimentally verified, representing the most extensive survey of any phytoplasma proteome to date. This study provided a valuable dataset of phytoplasma proteins, and a better understanding of the energy metabolism and virulence mechanisms of MD phytoplasma.

Citation

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posted Jul 9, 2010, 9:10 AM by rajesh gk

Title

Effects of Cina on root-knot disease of mulberry.

Authors

Datta SC.

 

Department of Zoology, Visva-Bharati University, Santiniketan 731235, West Bengal, India. dattasubhas@rediffmail.com

Journal

Homeopathy. 2006 Apr;95(2):98-102.

Abstract

Root-knot disease of mulberry is caused by the nematode Meloidogyne incognita. It has important economic implications for sericulture. The homeopathic medicines, Cina mother tincture (MT) and potentised Cina 200C, prepared from the flowering meristems of Artemisia nilagirica (Clarke) Pamp, were applied by foliar spray on mulberry (Morus alba L.) infected with M. incognita juveniles (J2). Pretreatment (ending 6 days before inoculation) and post-treatment (starting 6 days after inoculation) schedules were tested. The two uninoculated control batches were treated with the same procedure with Cina MT and Cina 200C. Both pre- and post-treatment significantly reduced nematode infection in terms of root gall number and nematode population in root. All the treated plants showed improved growth in terms of fresh biomass of shoot and root, length of shoot and root, number of leaves, leaf surface area, root and leaf-protein content. Cina 200C is more effective than Cina MT in all respects of nematode control as well as growth of the test plants. Pretreatments show slightly better effects than the post-treatments. It is interesting that inoculated and treated plants not only are less affected by nematodes but also have a better growth than uninoculated, untreated control.

Citation

http://www.ncbi.nlm.nih.gov/pubmed/16569626 PMID: 16569626 [PubMed - indexed for MEDLINE]

posted Jul 4, 2010, 6:10 AM by rajesh gk

Title

A new report of a mosaic dwarf viroid-like disease on mulberry trees in China. Free Article

Authors

Wang WB, Fei JM, Wu Y, Bai XC, Yu F, Shi GF, Li YF, Kuai YZ.

 

Institute of Life Science, Jiangsu University, Zhenjiang 212013, China.

Journal

Pol J Microbiol. 2010;59(1):33-6.

Abstract

A viroid-like disease causing mosaic leaves and dwarfism was found on mulberry plants in Zhejiang, China. Grafting of stems from infected plants onto healthy plants resulted in the same symptoms on the healthy plants. A circular small RNA (Mmd-v RNA1) was isolated from the infected plant leaves and caused identical symptoms after more than two years. Nucleotide sequencing indicated that the Mmd-v RNA1 contains 356 nt (GenBank accession no. NC_011362) without viroid characteristic regions, only sharing 30 nt sequence identity with that of the Cherry small circular viroid-like RNA 1 (isolate cscRNA1.150, GenBank accession no. DQ357628). This description is the first of viroid-like RNA infection of mulberry trees.

Citation

PMID: 20568527 [PubMed - in process]

posted Jul 4, 2010, 6:04 AM by rajesh gk   [ updated Jul 4, 2010, 6:12 AM ]

 

Title

Enterobacter spp.: A new evidence causing bacterial wilt on mulberry.

Authors

Zhu B, Wang G, Xie G, Zhou Q, Zhao M, Praphat K, Li B, Tian W.

 

State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou, 310029, China.

Journal

Sci China Life Sci. 2010 Feb;53(2):292-300. Epub 2010 Mar 7.

Abstract

Thirty-six pathogenetic bacterial strains were isolated from wilted mulberry plants in Hangzhou, Zhejiang province of China. The six representative strains were confirmed to be involved in more than one Enterobacter species by common bacteriological test, electron microscope observation, hypersensitive reaction, Koch's postulates, physiological and biochemical test, biolog, fatty acid methyl esters analysis (FAMEs), enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR), 16s rRNA sequences analysis, and comparative analysis with 7 type strains and 3 reference strains. This is the first report on mulberry disease caused by Enterobacter spp. in the world providing new evidence on induction of the plant disease in this genus. The results are not only important in the mulberry disease management but also have significant scientific value for further studies of opportunistic human pathogens and environmental strains in Enterobacter.

Citation

PMID: 20596840 [PubMed - as supplied by publisher]

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