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Genetics & Breeding

posted Mar 4, 2011, 8:09 AM by rajesh gk

Title

Diversification of mulberry (Morus indica var. S36), a vegetatively propagated tree sp ecies

Authors

Vijayan, K.*, Chatterjee, S.N., Nair, C.V

Journal

Caspian J. Env. Sci., 7, 23-30. ( 2009 )

Abstract

Genetic diversity among plants sprouted from seven year old clones of mulberry (Morus indica var. S36) was studied using molecular markers such as ISSR and RAPD. The clones showed significant variability in sex expression and the sprouting behavior. These changes were appeared after seven years of rigorous pruning and training (pruned once in every 3-4months). Genetic diversity analysis revealed significant DNA polymorphism differences among these clones. Through multiple regression analysis, four DNA markers associated with specific traits were identified. Cloning, sequencing and bioinformatics analyses suggested probable involvement of transposable elements along with enzymes and transcriptional regulating factors. The results of this preliminary report call for detailed analyses at biochemical, physiological and molecular levels coupled with inheritance pattern of these markers and their corresponding phenotypic traits. The markers identified for sprouting and sex expression are of much use in early identification of hybrids with the respective traits.

Citation

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Title

Comparative studies on crossability in some popular cultivars of mulberry (Morus Spp.)

Authors

Govinda Raju, M. V. and Basavaiah

Journal

Drug Invention Today; 2010; 2(2); p. 152-154

Abstract

The breeding system consists of mulberry (Morus spp.) cultivars such as Mysore local and Sujanpur 1 (Females) and S34, S13, DD, RFS 135, Ber.S1, Ber.C776 and V1 (males) were examined. In the month of March, the maximum seed set percentage ranges from 65.54 to 74.81 was observed in cross combinations using V1, S13, RFS135 as male parents. Whereas in September, the maximum seed set ranges from 62.82 to 68.40 was observed in cross combinations using Ber.S1, S13 and DD as male parents. The environment or a particular period such as temperature, humidity influenced on the rate of seed set. Pollen fertility is maximum in S34, S13, and V1 (97.0, 95.50 and 95.0). March month is better for crossability than in the month of September.

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Title

Molecular distinction amongst varieties of mulberry using RAPD and DAMD profiles. Free PMC Article

Authors

Bhattacharya E, Ranade SA.

PMB Division, National Botanical Research Institute, Rana Pratap Marg, Lucknow 226001 (UP) India. esha_b@rediffmail.com

Journal

BMC Plant Biol. 2001;1:3. Epub 2001 Dec 13.

Abstract

BACKGROUND: Mulberry trees are the most important host for rearing mulberry silkworms in sericulture. Improved varieties of mulberry tree have been developed through traditional breeding procedures. Not much work, however, has been carried out on the molecular characterization of these varieties. Random Amplified Polymorphic DNA (RAPD) and Directed Amplification of Minisatellite DNA (DAMD) methods based on Polymerase Chain Reaction are important tools to analyze genetic diversity of mulberries. These have been used to determine variation amongst nine varieties of Morus spp. maintained at Banthra Research Station of National Botanical Research Institute, Lucknow. RESULTS AND DISCUSSION: The varieties were analyzed using 23 arbitrary sequence decamer primers for RAPD, and 3 minisatellite core sequence primers for DAMD reactions. The RAPD and DAMD band data, (a total of 200 bands), were used to determine the pair wise distances according to Jaccard's algorithm. From these distance values Neighbour Joining (NJ) analyses were carried out separately for the RAPD and the DAMD data. The triploid varieties were found to be most similar to each other using RAPD analysis, while the varieties S13 and S34 were more similar using DAMD analysis. Nearly 85% of the RAPD bands and 91% of the DAMD bands were polymorphic across the nine varieties. CONCLUSIONS: The mulberry varieties could be distinguished by their RAPD and DAMD profiles. As many as five RAPD primers and one DAMD primer generated profiles that can together differentiate all the nine varieties in terms of unique bands.

Citation

http://www.ncbi.nlm.nih.gov/pubmed/11801190 PMID: 11801190 [PubMed - indexed for MEDLINE]PMCID: PMC64612

 

Title

DNA marker-assisted evaluation of cultivated and local mulberry genotypes of southern India

Authors

Keshava Murthy Bengaluru Channappa ; Bandekodigenahalli Marappa Prakash ; Shailaja Hittalmani ; Hosagavi Puttegowda Puttaraju

Journal

Crop Breeding and Applied Biotechnology (2009) 9 (3) 239-245

Abstract

Germplasm evaluation is essential in any crop improvement program and genetic characterization atmorphological and molecular level is very vital for breeding programs to be successful. Twenty six cultivated and localgenotypes of mulberry were subjected to diversity analysis with RAPD markers. Among the total 31 RAPD primers studied, 24were polymorphic and 7 were monomorphic. Of the total 197 loci obtained from 24 polymorphic primers, 110 loci (55.83%)were polymorphic and 87 (44.16%) were monomorphic. A clear grouping was seen among the cultivated genotypes based onyield with varieties like S36, V1, S54 and M5 showing proximity to each other and sharing a close similarity. Local genotypesare rich reservoirs of resistant gene sources and are well acclimatized to the prevailing environmental conditions.Utilizationof these along with other high yielding varieties will produce various combinations of resistance sources which can beincorporated into high yielding varieties.

Citation

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posted Jul 9, 2010, 9:07 AM by rajesh gk

Title

Genetic analysis of Indian mulberry varieties through molecular markers.

Authors

Vijayan K, Awasthi AK, Srivastava PP, Saratchandra B.

Seri-Biotech Research Laboratory, CSB Campus, Carmelram, Kodathi, Bangalore, Karnataka, India. kvijayan01@yahoo.com

Journal

Hereditas. 2004;141(1):8-14.

Abstract

India is one of the countries where sericulture is being practiced traditionally. Due to the higher economic return and the greater employment potential, attempts are being made to increase the productivity by developing high yielding mulberry varieties. At the present, Mysore local, Bomaypiasbari, Kanva-2, Bilidevalaya, Kajli, S1, BC(2)59, C776, RFS-175, S-36 and Victory-1 are being cultivated extensively in different parts of India for rearing the silkworm Bombyx mori L. Using 17 random amplified polymorphic DNA (RAPD) and 11 inter-simple sequence repeat (ISSR) primers the genetic relationships among these varieties were analyzed. The RAPD and ISSR primers revealed more than 75% polymorphism among the varieties. The genetic similarity estimated from RAPD markers varied from 0.645, between Kajli and Victory-1 to 0.887, between Kanva-2 and Bilidevalaya. Similarly, the genetic similarity estimated from the ISSR markers ranged from 0.600, between Kajli and Victory-1, to 0.873 between Kanva-2 and BC(2)59. The dendrogram constructed from these markers grouped the varieties into three major groups comprising the low yielding, medium yielding and high yielding. The low genetic similarity between the group of varieties originating from the eastern regions with that of the southern region encourages formation of extensive breeding programs between these groups as to transfer the high yield potential of the southern varieties to the low yielding but highly adaptive eastern varieties.

Citation

http://www.ncbi.nlm.nih.gov/pubmed/15383066 PMID: 15383066 [PubMed - indexed for MEDLINE]

 

Title

Mulberry improvements via plastid transformation and tissue culture engineering.

Authors

Umate P.

 

Department of Botany, Kakatiya University, Warangal, India. pavan_umate@rediffmail.com.

Journal

Plant Signal Behav. 2010 Jul 9;5(7). [Epub ahead of print]

Abstract

The in vitro tissue culture and micropropagation studies for Morus spp., a pivotal sericulture plant, are well established. The rapid and reproducible in vitro response to plant growth regulator treatments has emerged as an essential complement of transformation studies for this plant species. A major area of study is the use of protoplast culture and fusion techniques where advantages to mulberry improvement can be applied. The advancements in genetic transformation of mulberry are reviewed, and a section on strategy for transforming plastids (chloroplasts) of mulberry is included. A role for mulberry in "molecular farming" is envisioned. The conclusions and future prospects for improvement of this economically important tree species are proposed.

Citation

http://www.ncbi.nlm.nih.gov/pubmed/20495352 PMID: 20495352 [PubMed - as supplied by publisher]

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