Sanger Sequencing Service Options
Sanger Sequencing is based on sequencing genomic regions of interested of no more than 800bp. These sequences will be viewed with chromatograms.

Requisites for Sanger Sequencing & Fragment Analysis using the ABI-3130xl:
  • User is responsible to have read and agree to our Terms of Agreement.
  • If sending more than 16 reactions, use PCR strip tubes or PCR plates.
  • If sending less than 16 reactions, use PCR tubes. Do not send 1.5mL tubes.
  • If sequencing PCR products, send copy of agarose picture of product & product quantification using NanoDrop or Qubit.
  • Please fill out our Sample Submission Form and send it to us via e-mail. This form is mandatory and is located at the end of this page.
  • Naming Samples:
    • Name tubes using consecutive numbers.
    • In the Sample Submission Form, name samples with consecutive numbers, and keep a file with your sample names for reference. 
      • For example:
        If you have 3 samples, each sample sequenced Forward and Reverse, naming would be:
        1, 2, 3, 4, 5, 6,
        where 1-3 will be samples 1 to 3, Forward and 4-6 will be the same samples but sequenced with the Reverse primer.
  • If you need a quotation for Sequencing Services, please e-mail us at sgf.upr@gmail.com.
Updated Prices (2014)!
    Service Cost
    Full Service Sequencing (FSS) $ 5.00 per WELL
    Ready to Sequence Services (RSS) $ 4.50 per WELL
    Ready to Load (RTL) NEW! $ 2.00 per WELL
    Fragment Analyses (all) $ 3.00 per WELL

 

 Note: We charge every 16 wells: For example, 
 8 wells will be charged as 16; 24 wells will be
 charged as 32, etc.



Sequencing Services:
Full Service Sequencing (FSS)
For Full Service Sequencing, the user provides their quantified, pure PCR product and primer, each separately. SGF will be charged of purifying the PCR product and perform the sequencing reaction. Currently, the PCR purification method is Exo-SAP (see the "Protocols" tab for details) and the Sequencing Chemistry being used is the Big Dye Terminator v3 from BioAnalytical Instruments.

Ready to Sequence Service (RSS)
For Ready to Sequence Services, the user provides their quantified and purified PCR product (or miniprep product for plasmids), and primer. SGF will perform Cycle Sequencing accordingly.

Ready to Load (RTL)
For Ready to Load Services, the user must perform their sequencing reaction and post purification. Samples must be either resuspended in Formamide or dry. These samples must be on a semi-skirted 96 well plate. See Protocols Section for details regarding specific sequencing reagents required).

We currently provide the following primers and are included in the price.
M13F (-20, -21)
5' TGT AAA ACG ACG GCC AGT 3'
M13F (-41)
5' CGC CAG GGT TTT CCC AGT CAC GAC 3'
M13R (-27)
5' CAG GAA ACA GCT ATG AC 3'
M13R (-48)
5' AGC GGA TAA CAA TTT CAC ACA GG 3'
T3 Prom
5' AAT TAA CCC TCA CTA AAG GG 3'
T7 Prom
5' TAA TAC GAC TCA CTA TAG GG 3'
T7 Term
5' GCT AGT TAT TGC TCA GCG G 3'
SP6 Prom
5' TAC GAT TTA GGT GAC ACT ATA G 3'
16S rRNA F
5' AGA GTT TGA TCC TGG CTC AG 3'
16S rRNA R
5' ACG GCT ACC TTG TTA CGA CTT 3'
ITS 4
5' GGA AGT AAA AGT CGT AAC AAG G 3'
ITS 5
5' TCC TCC GCT TAT TGA TAT GC 3'
MatK F
5' TAA TTT ACG ATC AAT TCA TTC 3'
MatK R
5' ACA AGA AAG TCG AAG TAT 3'
rbcl F
5' ATG TCA CCA CAA ACA GAG ACT AAA GC 3'
rbcl R
5' GTA AAA TCA AGT CCA CCR CG 3'
FishF1
5' CAA CCA ACC ACA AAG ACA TTG GCA C 3'
FishF2
5' CGA CTA ATC ATA AAG ATA TCG GCA C 3'
FishR1
5' TAG ACT TCT GGG TGG CCA AAG AAT CA 3'
FishR2
5' ACT TCA GGG TGA CCG AAG AAT CAG AA 3'

Product Quantity and Quantification:
    Template Length Quantity/reaction
    100-200 bp 1-3 ng
    200-500 bp 3-10 ng
    500-1000 bp 5-20 ng
    1000-2000 bp 10-40 ng
    < 2000 bp 40-100 ng
    Plasmids 200-500 ng












Fragment Analysis Services
Please contact SGF Staff as every project varies.
We provide ROX Size Standard (included in the price).

REMEMBER:
READ SGF TERMS OF AGREEMENT

  • We charge by multiples of 16 reactions. 16-reactions are considered full runs. Partial runs are considered as full runs for payment processing.
  • Turn-around usually takes 24-72 hours depending on queue. Longer turn-around will always be notified to the user.

Two Options to Submit Samples:
  1. Ship samples to:
    University of Puerto Rico, Río Piedras
    Department of Biology
    Julio García Díaz building Room 118
    ATTN: Sequencing & Genotyping Facility (JGD-214)
    Ave. Ponce de León
    San Juan, PR 00931

  2. Personally Drop Off samples to:
    University of Puerto Rico, Río Piedras
    Julio García Díaz Room 214
     - First Freezer to the left
     - Second Shelf
     - White Box
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SGF UPRRP,
Jan 22, 2015, 7:57 AM