PACT Due Dates

Longitudinal Experiment -Plants


Plant germination with various covering materials

posted Feb 9, 2012, 5:28 PM by Dennis Yongmaneeratana   [ updated Mar 1, 2012, 4:38 PM by Kayla Rice ]

Question:  How will various covering materials affect the number of leaves of Lima bean plants over time?

Hypothesis:  If we plant Lima bean seeds under 3 different coverings of plastic wrap, aluminium foil, and none (control), then the seeds with the plastic wrap will grow the most leaves.

Independent variable:  Time
Dependent variable:  number of leaves
Series:  covering material

Materials:
3 plastic cups (with 5 small holes in bottom)
Organic Choice Potting Mix (Miracle Growth)
15 Lima Beans seeds
Water (30 mL for each set-up daily)
Aluminium Foil
Plastic Wrap
Graduated Cylinder
Dennis' balcony :-)
Kayla's Camera

Experimental Design:
Control:
1 cup with potting mix, one Lima bean, no covering.
Experiment 1 - Plastic wrap:
1 cup with potting mix, one Lima bean, covered with plastic wrap
Experiment 2 - Aluminium foil:
1 cup with potting mix, one Lima bean, covered with aluminium foil.

Procedure:
1.  Add approximately 45 grams of potting mix to each plastic cup.
2.  Plant five Lima beans in each cup, approximately 1 inch below the surface of the soil.
3.  Add 30 mL of water to each cup.
4.  Cover Experiment 1 with plastic wrap.  Cover Experiment 2 with Aluminium foil.  Leave the control experiment uncovered.
5.  Drive to Dennis' apartment and place them on the balcony.
6.  Water beans with 30 mL of water every other day by removing the cover, adding the water, and carefully coving the cup again.
7.  Take photos every other day.

Pictures:

Day 3 of experiment.

Day 6 of experiment.

Day 9 of experiment.

Day 12 of experiment.

Day 15 of experiment.

Day 18 of experiment.

   
Day 20 of experiment.                                                     Day 20 of experiment.

Day 21 of experiment.

Radish Seed Germination and Growth in Light vs Dark – Etiolation

posted Feb 9, 2012, 5:28 PM by Stephen Herr   [ updated Mar 1, 2012, 4:32 PM ]



Picture 1: Top row – light, Bottom Row – dark. 

Dark
Light




How will the presence of light affect radish seed germination and growth?

Materials: 
    6 petri dishes
    Radish seeds
    Water
    Sunlight and a dark place :)

Procedure:
    We placed 20 radish seeds in each container and distinguished 3 to be placed in an area exposed to natural sunlight and 3 to be      placed in an area which receives no sunlight. Radish seeds were given 10 drops of water each day and monitored for changes.  

    

Effect of Growth Light Enhancement on Rate of Germination and Early Growth of 3 Different Bean Species

posted Feb 9, 2012, 5:18 PM by Kevin Dixon   [ updated Mar 1, 2012, 5:11 PM ]





Group consists of Kevin Dixon, Mark Rothenay, and Tracy Foster.

Hypothesis:  Since Germination and early growth rely on stored food energy within the seed, we expect to see no initial growth difference between the two groups of seeds.  As the young seedlings begin to respirate and rely upon their own photosynthesis, we expect the group of seedlings with growth light enhancement to have accelerated growth.  We are also curious as to what difference we may see between the different seed species.

Setup:  Two identical trays with 12 seeds each, 4 of each variety (Pink, Pinto, and Lima) are prepared identically.  One tray is under an LED Growth light 24 hours per day, the other is on top of the LED light structure so it receives the same ambient light as the LED-enhanced tray.

Height Data after Two Weeks:
Ambient Light -- Pinto Bean -- Pink Bean -- Lima Bean
                              38.3 cm          29.4 cm          37.9 cm
Light Bank -- Pinto Bean -- Pink Bean -- Lima Bean
                        28.5 cm           5.93 cm         17.9 cm

While the ambient-only plants grew taller, the light-bank plants looked "healthier"; sturdier, darker green, larger leaf areas.

The darker green, shorter growth, and larger leaf areas are easily hypothesized to be the result of the extra light available to half of the plants.

Obviously, using height was not the best metric to measure the expected greater plant growth of the samples with extra light. 

After One Week:  

After Two Weeks:

Side-By-Side after Two Weeks:

Effect of Different Wavelengths of Light on Plant Height Growth (Natasha & Melissa)

posted Feb 9, 2012, 4:48 PM by Melissa Portillo   [ updated Mar 1, 2012, 4:11 PM ]

Question: What is the effect of different wavelengths of light on the plant height growth of raddish seeds.

Controls:
- length of light exposure
- seed quantity
-color filters

Materials: 4 small cups, raddish seeds, potting soil, blue, red, and clear cellophane.

Procedure:
- Make 4 holes on bottom of each cup
- plant 5 seeds/cup in 2" of soil
-soil and seeds were watered prior to covering with cellophane

-1 cup was covered with clear plastic
-1 cup was covered with red plastic
-1 cup was covered with blue plastic
-1 cup was covered with red and blue plastic

-The 4 cups were placed near a window to receive natural light during the day (about 11 hours of sunlight/day).
-The 4 cups were placed in a tray of water to keep the soil moist during the week.

Observations 2/16/12

- There are two sprouts from the clear cellophane container. Growth of each is 1.5"
- The container with red cellophane had one sprouted plant about 2" high.
- The container with the blue cellophane had one sprout with growth of about 3" (growth outside of rim of cup)
- The container with both blue and red cellophane had two sprouts.  The heights of both was a little more than the sprout in the blue cellophane container (also outside rim of cup).
- The water had completely been depleted within a week and new water was added to the tray.


Observations on 2/23/12:

-All 4 cups had seeds that had sprouted
- The sprout in with the clear cellophane wrapped around it had showed the most growth in height.
- The tray had to be replenished with water as there was none left.


Effect of scarification and soaking on germination time in beans

posted Feb 9, 2012, 4:46 PM by Jacob Bear   [ updated Feb 9, 2012, 5:22 PM by Craig Marsden ]

Independent variable = time
Dependent variable = germination 

Plant: Pinto Bean

Three series of seed treatments:
1. No treatment
2. Seed coat physically cut with knife.
3. Seeds soaked.

Two cups of six seeds per cup in potting soil. 
Cups grown in two locations of bright indirect light indoors.
Soil kept moist at all times.

Effect of Light on Direction of Lima Bean Growth Over Time

posted Feb 9, 2012, 4:44 PM by Rita Faynerman   [ updated Mar 1, 2012, 4:45 PM ]

Researchers: Monica Platten, Rita Faynerman

Hypothesis: If lima beans are grown under a light source, then the plant will grow towards the direction of the light.

Materials: Lima bean seeds, planting soil, growth trays, LED light source, water.

Independent Variable: Direction of plant growth 

Dependent Variable: Time

Series: Light source from 3 different directions (left, right, and directly overhead)

Procedure:  We will place 3 different lima bean seedlings in different positions relative to the light source.  Plant A will be placed to the left of the light source, plant B will be placed directly underneath the light source, and plant C will be placed to the right of the light source.  We will then qualitatively note the direction of the plant growth relative to a line running perpendicular to the surface on which the plant is growing. 
 
Results:  After three weeks, each plant did grow in the direction of the light.  Plant A, which was placed to the left of the light source, grew toward (curved toward) the right.  Plant B, which was underneath the light source, grew straight up (did not curve).  Plant C, which was placed to the right of the light source, grew toward (curved toward) the left.
 
Pictures:
 
Plant A
Plant A


Plant B
Plant B
 
Plant C
Plant C

Mara and Jamie's Diet Coke Adventure!!

posted Feb 9, 2012, 4:43 PM by Jamie Ryan   [ updated Mar 1, 2012, 4:37 PM by Tamara Desso ]

We will "water" three different types of seeds with diet coke once a week, and measure the growth response in the height of sprouts.

Independent variable: time
Dependent variable: height
Series: 3 different types of seeds

Control: watered with water once a week (same amount in mL as coke)
Experimental group: diet coke


Above: Pink, Lima, Pinto given diet coke every 7 days for 3 weeks.
Below: Pink, Lima, Pinto given water every 7 days for 3 weeks.


Effect of Differing Light Conditions on Lima Bean Height over Time

posted Feb 9, 2012, 4:42 PM by newmankhs@gmail.com   [ updated Feb 9, 2012, 5:25 PM ]

Effect of differing light conditions (grow lights, natural sunlight, no light) on plant height over time.
 
Researchers:  Jason Newman, Katrin Kahen, Daniel Prasch
 
Hypothesis:  Because solar radiation includes harmful elements as well as beneficial light, we believe that we will see an increase in growth over time under the LED lights as compared to natural sunlight.  We expect the no light situation to produce very little if any growth.
 
Materials:  Beans (single variety), planting soil, planting tray, grow lights, water, tinfoil
 
Setup:  Three groups of beans, planted in the same amount of soil and given the same amount of water via capillary action with water in a tray.  Group A will be exposed to natural sunlight during normal daylight hours; Group B will be exposed to grow lights 24 hours a day; Group C will be exposed to No light at all.  This will persist for 2 weeks.  Each day, the height of the plants will be measured and data collected.

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