Regional Animal Disease Diagnostic Laboratory

Brief Description/History/Implementing Guidelines

The Regional Animal Disease Diagnostic Laboratory provides services that are vital to the development of the livestock and poultry industry in region 3 and nearby provinces. It performs prompt and accurate diagnostic tests of major animal diseases that serve as basis for the therapeutic, prophylactic and eradication measures of disease outbreaks.

Our laboratory also provides tests in support to trade (local, export and import), disease surveillance, animal health, regulatory and research. Furthermore, our facilities serve as training area for Veterinary clinicians, students and professionals as well.

The laboratory was established in 1986 as an imperative component of a disease surveillance system and in the overall design of an animal health program for Central Luzon.

Goal: To provide diagnostic services necessary for the therapeutic, prophylactic and elimination of animal disease cases.

Objectives:

  1. Provides prompt and accurate diagnosis of major diseases of poultry, livestock and pet animals as basis for the recommendation of treatment, control and prevention of diseases.

  2. Performs laboratory tests in support to animal health, surveillance, regulatory and research.

  3. Serve as training facilities of students in terms of animal disease diagnosis.

Program Components/Services

        RADDL is a government laboratory that provides services in support to animal health and production, regulatory, research and surveillance. It offers the tests from Monday thru Friday at 8 am to 5 pm. The services available are as follows:

Objectives:

  1. Provides prompt and accurate diagnosis of major diseases of poultry, livestock and pet animals as basis for the recommendation of treatment, control and prevention of diseases.

  2. Performs laboratory tests in support to animal health, surveillance, regulatory and research.

  3. Serve as training facilities of students in terms of animal disease diagnosis.

 

Program Components/Services

            RADDL is a government laboratory that provides services in support to animal health and production, regulatory, research and surveillance. It offers the tests from Monday thru Friday at 8 am to 5 pm. The services available are as follows:

  1. PATHOLOGY

    1. Gross Pathology/Necropsy

    2. Hematology (available in year 2016)


  1. VIROLOGY

    1. Antigen Detection

      1. Rabies Examination- DME &FAT

      2. Hog Cholera Examination – FAT, RT-PCR

      3. Avian Influenza – RT-PCR

      4. Swine Flu – RT-PCR

      5. PRRS – RT-PCR

    2. Antibody Detection

      1. Avian Influenza – HI Test

      2. PRRS – ELISA

      3. Newcastle disease – HI Test

      4. Caprine Arthritis Encephalitis – ELISA

      5. Pseudorabies – ELISA


  1. BACTERIOLOGY

    1. Antibiotic Sensitivity Test

    2. Bacterial Isolation and Identification

    3. Fungal Isolation and Identification

    4. Salmonella Isolation in feeds and tissue

    5. Rapid Plate test for  Brucella abortus

    6. Tuberculin test for Mycobacterium

    7. Mycoplasma hyopneumonia-PCR and LAMP

    8. Isolation of Actinobacillus pleuropneumoniae and Heamophilus parasuis

    9. Water coliform count


  1. PARASITOLOGY

    1. Blood Parasite Examination

      1. Direct Smear

      2. Stained Smear

      3. Mice Inoculation

    2. Fecal Examination

      1. Direct smear

      2. Floatation

      3. Sedimentation

      4. Larval Culture

      5. Egg count

    3. Identification of Parasite

    4. Artificial Digestion

    5. Skin Scraping

 

WHO MAY AVAIL OF THE SERVICE

    • Livestock Farmers
    • Poultry Raisers
    • Traders – exporters, importers, local traders
    • Veterinarians
    • Livestock Technicians
    • Researchers
    • Clinicians
    • Students
    • Consumers
    • Government employees
    • Pet owners
    • Cooperatives
    • Local government units
    • Other agencies

 

REQUIREMENTS TO BE BROUGHT OR SUBMITTED

Pathology

Best animal for necropsy

    • Sick animals showing signs of the disease (early, advance stage and apparently healthy)

       

Virology

    • Dog’s head for rabies examination should be frozen, chilled or keep under cold condition until it reaches the laboratory. Never preserve with formalin.
    • Organs or tissues at least 2 x 2 inches from the lungs, liver, heart, spleen, kidney, lymph nodes, brain, intestines
    • Swabs (cloacal, oropharengeal, tracheal) for antigen detection should be placed in transport media, frozen or chilled until it reach the laboratory.
    • Whole blood samples should be kept under ref condition
    • Blood for serum should be kept under room temperature until 12-24 hours before separating the serum to avoid hemolysis

           

Bacteriology

    • Tissues should be kept under ref condition until it reach the laboratory
    • Tissues  taken after field necropsy should be placed in separate container and in cold condition then submit immediately to the lab.
    • Don’t freeze the samples

Parasitology

    • For blood parasite examination:  submit whole blood with anticoagulant.
    • Fecal samples should be kept under cold condition until they reach the laboratory and never freeze
    • Whole parasite for identification should be placed in 70 % alcohol
    • Blood smears should be fixed with methanol when not submitted immediately to the laboratory.

 

DURATION OF THE SERVICE AND SCHEDULE OF FEES


 TYPE OF SERVICES

DURATION UNDER NORMAL CONDITION

WITH RESULT

 SCHEDULE OF FEES
(Php)

PATHOLOGY

Necropsy

Poultry up to 8 weeks old

1 day

50.00 / head

Poultry over 2 months old

1 day

75.00 / head

Swine up to 8 weeks old

1 day

100.00 / head

Swine over 2 months old

1 day

150.00 / head

Swine sow/boar

1 day

200.00 / head

Goat

1 day

150.00 / head

Cattle/carabao/horse up to 8 weeks

1 day

200.00 / head

Cattle/carabao/horse over 2 months old

1 day

300.00 / head

Hematology – by 2016

RBC/HGB/HCT/PLT

1 day

 

WBC/LY/MO/GR/EO/

1 day

 

VIROLOGY

Antigen Detection

Rabies Examination DME/FAT

1 day

Free until 2016

Hog Cholera FAT

1 day

P 200 / sample

Hog Cholera RT PCR

2 days

3000 / pool of 5 samples

Avian Influenza RT PCR

2 days

3000 / pool of 5 samples

Swine Influenza

2 days

3000 / pool of 5 samples

PRRS

2 days

3000 / pool of 5 samples

Antibody Detection

ND HI test

2 days

75.00/sample

Avian Influenza

2 days

100.00/sample

CAE ELISA

2 days

300.00/sample

Pseudorabies ELISA

2 days

300.00/sample

PRRS ELISA

2 days

300.00/sample

BACTERIOLOGY

Bacterial isolation and Identification       

2-3 days

250.00/case

Antibiotic Sensitivity Test

2-3 days

150.00/case

Salmonella isolation in feeds and tissues

4-5 days

450.00/sample

Fungal Isolation and identification

4-5 days

250.00/sample

Brucella abortus RPT

1 day

150.00/sample

Mycobacterium tuberculin test

3 days

 

Isolation of APP and Hemophilus

2-3 days

250.00/sample

Mycoplasma hyopneumoniae PCR

2-3 days

3000.00/pool of 5

Mycoplasma hyopneumoniae LAMP

2 days

300.00/pool of 5

Pasteurella multocida PCR

2-3 days

1000.00/pool of 5

Water coliform count

4-5 days

300.00/sample

PARASITOLOGY

Blood Parasite Examination

Direct Smear

1 day

15.00/sample

Stained Smear

1 day

25.00/sample

Mice Inoculation test

5-7 days

60.00/sample

Fecal Examination

 

 

Direct smear

1 day

15.00/sample

Floatation

1 day

20.00/sample

Sedimentation

1 day

20.00/sample

Larval Culture

7 days

35.00/sample

Egg count

1 day

50.00/sample

Skin Scraping

1 day

25.00/sample

Parasite Identification

 

 

Tissue parasite

2 days

170.00/sample

Whole parasite

1 day

50.00/sample

 

PERSON TO BE CONTACTED FOR INQUIRIES

RADDL landline – (045) 961-2934

DR. MILAGROS R. MANANGGIT
Veterinarian IV

DR. CORNELIO VELASCO
Veterinarian III

DR. DENISE ANN DAYAO
Veterinarian II

DR. XANDRE BACCAY
Veterinarian II

DR. JOELY ONGTANGCO
Veterinarian

DR. AGNES DELA CRUZ
Veterinarian

MS. JULICET CARPIO
Lab. Technician

 

HOW TO AVAIL OF THE SERVICE

STEP CLIENT SERVICE PROVIDER PROCESSING TIME
(UNDER NORMAL 
CIRCUMSTANCE)
PERSON IN-CHARGE
1. Submit samples, fill-up submission and client feedback forms Interview, encode data in record book, verify forms if data is complete, compute lab payment 10 mins. Ms. J. Carpio
Mr. de Guzman
Dr. Mananggit
2. Pay laboratory fee to the cashier/collecting officer Bring samples to the designated room for testing & check if samples are fit for the test 5 minutes Mr. Mangalus
Dr. Velasco
3. Present the receipt for recording Encoding of receipt no. and amount to the record book 2 mins Ms. J. Carpio
Mr. de Guzman
4. Testing of samples 1-5 days depending on the tests requested Dr. Mananggit
Dr. Velasco
Dr. Baccay
Dr. Ongtangco
Dr. dela Cruz
5. Claim the result personally or thru email Preparation of result 10 minutes Ms. J.Carpio
Mr. de Guzman
6. Consultation to the veterinarian Discuss results and give recommendations 20 minutes Dr. Mananggit
Dr. Baccay
Dr. Ongtangco
Dr. dela Cruz
END OF TRANSACTION


APPENDIX

Downloadable forms: