Separation Science (分離科學)


The course is directed to graduate students who specialize or are interested in analytical, pharmaceutical, or clinical chemistry, as well as biochemistry, biotechnology, systems biology, and related areas.

It will provide a comprehensive overview of separation science-related technology, methodology, and applications.


Contents (by topic):

1. Introduction

2. Analytical extractions

3. Gas chromatography

4. Liquid chromatography

5. Electrophoresis


6. * Mid-term exam


7. Miniaturization of separations


8. Automation of separations


9. Hydrodynamic separations of particles

10. Ion-mobility spectrometry


11. * Students’ presentations


12. Selected applications


13. * Final exam



* Final mark will be based on the results of the mid-term exam (30%), presentation (30%), and the result of the final exam (40%). Additional points (up to 15%) can be gained for active participation in the class.



Students who have completed the Analytical Chemistry course are encouraged to participate.


Study material:

Handouts will be provided for selected topics.


1) Lundanes E., Reubsaet L., Greibrokk T. 2014, Chromatography. Basic Principles, Sample Preparations and Related Methods. Wiley-VCH, Weinheim.

2) Dettmer-Wilde K., Engewald W. (eds.) 2014, Practical Gas Chromatography. A Comprehensive Reference. Springer, Berlin.

3) Fanali S., Haddad P.R., Poole C.F., Riekkola M.-L. (eds.) 2017, Liquid Chromatography. Volume 1. Fundamentals and Instrumentation. 2nd Edition. In: Poole C.F. (series ed.) Handbooks in Separation Science. Elsevier, Amsterdam.

4) Westermeier R. 2005, Electrophoresis in Practice: A Guide to Methods and Applications of DNA and Protein Separations. 4th, Revised and Updated Edition. Wiley-VCH, Weinheim.

5) Eiceman G.A., Karpas Z., Hill Jr. H.H. 2014, Ion Mobility Spectrometry. Third Edition. CRC Press, Boca Raton.

Office hour:

Time: Friday, 14:00-15:00.

Place: room 316

Useful links: (This section will be expanded.)


Handouts (for the participants of this course only):

The handouts and other course materials are currently available in the NTHU iLMS website. You have to log in.

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