Urban Lab

The newly developed analytical method allows researchers to study protein folding in response to rapidly changing pH. An autocatalytic enzymatic reaction is employed to ramp up pH. At the same time, alterations to target protein conformation are monitored by mass spectrometry or fluorometry. [read more]

Telechemistry enables remote control of chemistry experiments and data logging via Internet cloud. Setting up remotely controlled experiments is straightforward with the Particle Photon Wi-Fi module. [read more]

Dual sample chamber mass spectrometry system has been developed to follow the release of volatile organic compounds (VOCs) from various organic specimens. The method enables monitoring VOC emanations that accompany microbial growth, fungal morphogenesis, and putrefaction. [read more]

Dry ice fog extraction is introduced for analysis of volatile organic compounds emanating from solid and liquid samplesAerosol microdroplets are generated by inserting dry ice into the extraction solvent. The resulting fog is directed toward the sample headspace, where gas-liquid extraction occurs. The microdroplets—containing the extracted compoundssubsequently coalesce on a cold surface providing a clean extract ready for analysis by chromatography or mass spectrometry. [read more]

Our lab develops hyphenated methods, in which sample preparation steps are coupled on-line with mass spectrometry. Recently continuous liquid-liquid extraction was coupled with atmospheric pressure chemical ion source of triple quadrupole mass spectrometer, and used in analysis of red wine. [read more]

In the most recent study we discovered that ramping up ATP (firefly luciferase substrate) leads to a change in color of emitted light. This observation has implication on the use of assays based on luciferase luminescence. [read more]

Diluting samples is no longer a manual operation. The plug-volume-modulation (PVM) system developed in our lab dilutes samples on demand following the programmed dilution ratio. [read more]

Imaging-based microgasometry enables detection of enzymatic activities in microliter-range samples. Reaction progress is determined based on the size of nanoliter bubbles produced in an oil matrix. [read more]
Fizzy extraction takes advantage of the effervescence phenomenon to extract volatiles from the liquid to the gas phase. The entire fizzy extraction process has been automated by using inexpensive microcontroller modules. The functions of this automated device include triggering the analysis by a smartphone app, control of carrier gas pressure in the headspace of the sample chamber, displaying experimental conditions on an LCD screen, acquiring mass spectrometry data in real time, filtering electronic noise, integrating peaks, calculating the analyte concentration in the extracted sample, printing the analysis report, storing the acquired data in non-volatile memory, monitoring the condition of the motor by counting the number of extraction cycles, and cleaning the elements exposed to the sample. [read more]

The technique of fizzy extraction (invented in our laboratory three years ago) has been coupled with gas chromatography. This hyphenated approach enables fast, sensitive, and selective analysis of volatile organic compounds (VOCs) present in various types of liquid samples. [read more]

We have developed a flow rate scanner comprising a pump, an inexpensive single-board computer, and an associated Python program. The device enables fast characterization of on-line detection systems (e.g. mass spectrometers). [read more]

A simple method has been developed to probe residues of volatile organic compounds present on skin surface. It takes advantage of inexpensive oil control blotting paper, which is used as the sampling probe. [read more]

We disclose a method to extract volatile organic compounds from the gas phase and transfer them to the liquid phase for further analysis. It takes advantage of high surface-to-volume-ratio of aerosol microdroplets[read more]


We introduced "fizzy extraction" for seamless analysis of volatile and semivolatile solutes in real samples. It works just as what you can see when you open a shaken bottle of soda. At the beginning of the extraction sequence, carbon dioxide is dissolved while stirring the sample. Then, the pressure is lowered to induce bubbling. Chemical molecules are released to the gas phase by effervescence. They are immediately transferred to the mass spectrometer for instantaneous detection. [read more]

Biochemical timer
We also disclosed a biochemical timer reaction generating luminescence bursts.

The time of luminescence (not the intensity!) depends on the initial concentration of the trigger molecule (ATP). [read more]

Hydrodynamic chromatography has been coupled online with electrospray ionization mass spectrometry to analyze liposome and cell suspensions in concentrated salt solutions with no sample preparation. [read more]