Urban Lab

Dual sample chamber mass spectrometry system has been developed to follow the release of volatile organic compounds (VOCs) from various organic specimens. The method enables monitoring VOC emanations that accompany microbial growth, fungal morphogenesis, and putrefaction. [read more]

Dry ice fog extraction is introduced for analysis of volatile organic compounds emanating from solid and liquid samplesAerosol microdroplets are generated by inserting dry ice into the extraction solvent. The resulting fog is directed toward the sample headspace, where gas-liquid extraction occurs. The microdroplets—containing the extracted compoundssubsequently coalesce on a cold surface providing a clean extract ready for analysis by chromatography or mass spectrometry. [read more]

Our lab develops hyphenated methods, in which sample preparation steps are coupled on-line with mass spectrometry. Recently continuous liquid-liquid extraction was coupled with atmospheric pressure chemical ion source of triple quadrupole mass spectrometer, and used in analysis of red wine. [read more]

In the most recent study we discovered that ramping up ATP (firefly luciferase substrate) leads to a change in color of emitted light. This observation has implication on the use of assays based on luciferase luminescence. [read more]

Diluting samples is no longer a manual operation. The plug-volume-modulation (PVM) system developed in our lab dilutes samples on demand following the programmed dilution ratio. [read more]

Imaging-based microgasometry enables detection of enzymatic activities in microliter-range samples. Reaction progress is determined based on the size of nanoliter bubbles produced in an oil matrix. [read more]

Using the hydrogel micropatch sampling method, skin excretion specimens obtained from 200+ patients and healthy individuals have been screened. The obtained mass spectra reveal numerous metabolites characteristic for a skin disease. The study demonstrates the possibility to conduct non-invasive and rapid metabolic profiling of skin excretions. Mass spectrometry is a convenient readout technique for the hydrogel micropatch probes exposed to skin. [read more]

Real-time mass spectrometry facilitates optimization of multi-enzyme biochemical circuits to stabilize adenosine nucleotide levels during biosyntheses. [read more]
We have developed a flow rate scanner comprising a pump, an inexpensive single-board computer, and an associated Python program. The device enables fast characterization of on-line detection systems (e.g. mass spectrometers). [read more]

A simple method has been developed to probe residues of volatile organic compounds present on skin surface. It takes advantage of inexpensive oil control blotting paper, which is used as the sampling probe. [read more]

We disclose a method to extract volatile organic compounds from the gas phase and transfer them to the liquid phase for further analysis. It takes advantage of high surface-to-volume-ratio of aerosol microdroplets[read more]


We introduced "fizzy extraction" for seamless analysis of volatile and semivolatile solutes in real samples. It works just as what you can see when you open a shaken bottle of soda. At the beginning of the extraction sequence, carbon dioxide is dissolved while stirring the sample. Then, the pressure is lowered to induce bubbling. Chemical molecules are released to the gas phase by effervescence. They are immediately transferred to the mass spectrometer for instantaneous detection. [read more]

Biochemical timer
We also disclosed a biochemical timer reaction generating luminescence bursts.

The time of luminescence (not the intensity!) depends on the initial concentration of the trigger molecule (ATP). [read more]

Hydrodynamic chromatography has been coupled online with electrospray ionization mass spectrometry to analyze liposome and cell suspensions in concentrated salt solutions with no sample preparation. [read more]

RAMSAY-2 has been developed to enable serial automated processing of samples prior to mass spectrometric analysis. [read more]