Urban Lab

Diluting samples is no longer a manual operation. The plug-volume-modulation (PVM) system developed in our lab dilutes samples on demand following the programmed dilution ratio. [read more]

Imaging-based microgasometry enables detection of enzymatic activities in microliter-range samples. Reaction progress is determined based on the size of nanoliter bubbles produced in an oil matrix. [read more]

Using the hydrogel micropatch sampling method, skin excretion specimens obtained from 200+ patients and healthy individuals have been screened. The obtained mass spectra reveal numerous metabolites characteristic for a skin disease. The study demonstrates the possibility to conduct non-invasive and rapid metabolic profiling of skin excretions. Mass spectrometry is a convenient readout technique for the hydrogel micropatch probes exposed to skin. [read more]

Micropatch-arrayed pads (MAPA) are introduced as a facile way to probe distributions of topical drugs on the surface of skin. [read more]

Real-time mass spectrometry facilitates optimization of multi-enzyme biochemical circuits to stabilize adenosine nucleotide levels during biosyntheses. [read more]
Fizzy extraction

We introduced "fizzy extraction" for seamless analysis of volatile and semivolatile solutes in real samples. It works just as what you can see when you open a shaken bottle of soda. At the beginning of the extraction sequence, carbon dioxide is dissolved while stirring the sample. Then, the pressure is lowered to induce bubbling. Chemical molecules are released to the gas phase by effervescence. They are immediately transferred to the mass spectrometer for instantaneous detection. [read more]

Biochemical timer
We also disclosed a biochemical timer reaction generating luminescence bursts.
The time of luminescence (not the intensity!) depends on the initial concentration of the trigger molecule (ATP). [read more]

Hydrodynamic chromatography has been coupled online with electrospray ionization mass spectrometry to analyze liposome and cell suspensions in concentrated salt solutions with no sample preparation. [read more]

RAMSAY-2 has been developed to enable serial automated processing of samples prior to mass spectrometric analysis. [read more]