Molecular Biology Proficiencies

General Techniques

Genetic

  • Polymerase chain reaction (PCR)
    • Primer design
    • Optimization
  • Sequencing
  • Genotyping
    • Pyrosequencing
    • TaqMan
  • Gene cloning
    • E.Coli transformation and manipulation
    • Single colony PCR
    • Miniprep and maxiprep
    • Restriction digest
    • Subcloning
    • TA cloning

Gene Expression

  • Quantitative real-time PCR (qRT-PCR)
    • Sybr-Green
      • Primer design
      • Optimization
    • TaqMan
  • Gene expression microarray
    • RNA quality checking
    • Probe synthesis
    • Data normalization and cleanup
    • Analysis
  • Luciferase Reporter Assays
    • Cloning
    • Transfection
    • miRNA mimics
    • miRNA inhibitors
    • Analysis

Protein Expression

  • Western blotting

Mammalian

Tissue Culture

  • Passaging cells
  • Cryo preservation
  • Transfection
  • DNA/RNA/Protein extraction

Tissue Samples

  • Homogenizing
  • RNA extraction (Fat biopsies)

Plant

  • DNA extraction (Leaf)
  • RNA extraction (Ovule and whole seedling)
  • Transformation using agrobacterium
  • Transformant selection and screening
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