These pages represent the research interests of Rainer Heintzmann with research groups at the Institute of Photonics Technology, the Department of Physical Chemisty at the FSU, Jena and the Randall Division of Cell and Molecular Biophysics, King's College London.
The research focuses on developing (often high-resolution) techniques to measure multidimensional information in small biological objects such as cells, cellular organelles, molecules or other structures of interest.
Molecules interact in living cells at specific places (e.g. inside organelles) and often at well defined times (e.g. after stimulation with other molecules). Much biological research is now focused onto unravelling these details. Luckily there are a number of physical effects, which can be used to tell things apart, when one or multiple molecules are fluorescing (either by themselves or by having a specific fluorescent molecule (fluorophore) attached to them).
For a nice demonstration on different biological objects and their sizes, click here.
Recently a number of molecules have been found that can be switched between different fluorescent states by illumination at separate wavelengths. The transitions between the states can be driven into saturation. The arising non-linear dependencies offer the unique possibility of a theoretically unlimited optical resolution. Part of our work focuses on an experimental demonstration of high resolution based on these effects. To this aim we employ an imaging mode in which the sample is illuminated with a grid of very fine lines. This technique is called structured illumination (SI).
This is an example comparing stuctured illumiination (left) and standard imaging (right) of muscle myofibrils acquired by Liisa Hirvonen in collaboration with Elisabeth Ehler.