The research focuses on developing (often high-resolution) techniques to
measure multidimensional information in small biological objects such
as cells, cellular organelles, molecules or other structures of
Molecules interact in living cells at specific places
(e.g. inside organelles) and often at well defined times (e.g. after
stimulation with other molecules). Much biological research is now
focused onto unravelling these details. Luckily there are a number of
physical effects, which can be used to tell things apart, when one or
multiple molecules are fluorescing (either by themselves or by having a
specific fluorescent molecule (fluorophore) attached to them).
For a nice demonstration on different biological objects and their sizes, click here. Recently a number of molecules have been found that can be switched
between different fluorescent states by illumination at separate
wavelengths. The transitions between the states can be driven into
saturation. The arising non-linear dependencies offer the unique
possibility of a theoretically unlimited optical resolution.
Part of our work focuses on an experimental demonstration of high
resolution based on these effects. To this aim we employ an imaging
mode in which the sample is illuminated with a grid of very fine lines.
This technique is called structured illumination microscopy (SIM). This is an example comparing stuctured illumiination (left) and standard imaging (right) of muscle myofibrils acquired by Liisa Hirvonen in collaboration with Elisabeth Ehler.
Selected Recent Publications
- R. Horstmeyer, R. Heintzmann, G. Popescu, L. Waller and C. Yang, Standardizing the resolution claims for coherent microscopy, Nature Photonics 9, 68-71, 2016
- H.W. Lu-Walther, M. Kielhorn, R. Förster, A. Jost, K. Wicker, R. Heintzmann, FastSIM: a practical implementation of fast structured illumination microscopy, Methods Appl. Fluoresc. 3 014001 doi:10.1088/2050-6120/3/1/014001, 2015
- K. Wicker, R.
Heintzmann.Resolving a misconception about structured illumination. Nature Photonics 342, 2014.
K. Wicker, O.
Mandula, G. Best, R.
Heintzmann. Phase optimisation for structured illumination microscopy. Optics Express 2032, 2013.
Cox, E. Rosten, J. Monypenny, T. Jovanovic-Talisman, D. T. Burnette, J. Lippincott-Schwartz,
G. E. Jones und R.Heintzmann. Bayesian
localization microscopy reveals nanoscale podosome dynamics. Nature
Methods 9, 195-200 , 2012.
- R. Heintzmann, Correcting distorted optics: back to the basics, Nature Methods 7, 108-110, 2010.
- R. Heintzmann, M.G.L. Gustafsson, The requirements for
sub-diffraction resolution in continuous samples, Nature Photonics 3, 362-364,
Hoppe, S. L. Shorte, J. A. Swanson, R. Heintzmann, D-FRET
Reconstruction Microscopy for Analysis of Dynamic Molecular
Interactions in Live Cells, Biophysical Journal 95, 400-418, 2008.
- K. Wicker and R. Heintzmann, Interferometric resolution improvement for confocal microscopes, Optics Express 15, 12206- 12216, 2007.
- R. Heintzmann and P.A. Benedetti, High-Resolution Image Reconstruction in Fluorescence Microscopy with Patterned Excitation, Applied Optics, 45, 5037-5045, 2006.
Lidke, P. Nagy, R. Heintzmann, D.J. Arndt-Jovin, J.N. Post, H. Grecco,
E.A. Jares-Erijman and T.M. Jovin. Quantum dot ligands provide new
insights in receptor-mediated signal transduction. Nature Biotechnology, 22, 198-203, 2004.
- R. Heintzmann., K.A. Lidke and T.M. Jovin. Double-pass Fourier transform imaging spectroscopy. Optics Express. 12, 753-763, 2004.
- R. Heintzmann and C. Cremer. Axial tomographic confocal fluorescence microscopy, J. Microsc., 206, 7-23, 2002.
Heintzmann, T.M. Jovin and C. Cremer. Saturated patterned excitation
microscopy (SPEM) - a novel concept for optical resolution improvement.
J. Opt. Soc. Am. A, 19, 1599-1609, 2002.
For a complete list see Publications