Project title: Microfluidic - Plasmonic Biosensor for Real Time Detection of Relevant Biomarkers         
Project Code: PN-II-PT-PCCA-2013-4-1961
Contract No:
39/1.07.2014
Budget:
1.437.500 lei (320.000 euro)
Financing:
Romanian National Authority for Scientific Research, UEFISCDI
Period:
  july 2014-september 2017
Project Leader:
Monica Focsan, PhD

Contact: monica.iosin@phys.ubbcluj.ro, tel: +4 0264454554/116

Abstract
       The project proposes the development of an optical, ultrasensitive, robust sensor for the detection of specific disease biomarkers in order to enable early diagnosis, improve diseases treatment, increase the overall survival and  diminish societal costs.  Our sensor is  based on the exploitation  of optical  response of plasmonic nanostructures known  as  Localized Surface Plasmon Resonance (LSPR) since this technique  has  already demonstrated its ability for a label - less highly  sensitive  molecular detection. The originality  of  the sensor lies in  the integration of  optimized plasmonic substrate in a microfluidic circuit  that allows miniaturization, portability and minimizing of the analysis time. Colloidal nanoparticles attached on solid substrate and/or nanostructured metallic film will be integrated in microfluidic channels and the LSPR detection process will be triggered by molecular recognition of biomarkers.

         The core task of the project will consist in optimizing the sensor parameters in terms of high sensitivity, selectivity and reproducibility. For this, optimization of the nanoparticles’ size and shape, optical and chemical properties of substrate, functionalization and bioanalyte recognition will be performed. The unique characteristics of our Microfluidic - Plasmonic Biosensor for Real Time Detection of Relevant Biomarkers will provide competitive advantages compared to other detection systems currently on the market (standard SPR, immunofluorescence and ELISA assays) and will promote our sensor as a good candidate for technology transfer. Indeed, in this project, we will take all the advantages of current developments in nano-optics and spectroscopy, nanotechnology and surface nanostructuration and Lab-On-a-Chip technologies to design a nanosensor suitable for the identification and detection of specific chemical or biological species in biological fluids. At the end of the project, we will be able to provide an optimized laboratory prototype with unique capabilities and diversified palette of future applications.



     

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