Mahesh Shantappa Dharne

Senior Scientist,
National Collection of Industrial Micro-organisms (NCIM)
National Chemical Laboratory (CSIR-NCL)
Dr. Homi Bhabha Road, Pashan
Pune 411 008, Maharashtra, INDIA

E Mail-
Phone (Lab) +91-20-25902450
Fax- +91-20-25902671

Research areas-Environmental Microbiology and Biotechnology

Theme: OMIC tools to explore microbial diversity for biotechnological applications
Major Activities- NCIM Resource Centre

For training/ project programs/ PhD admission/ postdoc positions in the laboratory: I welcome enthusiastic master students (JRF qualified/not qualified) and doctoral fellows (Biotechnology, Microbiology, Bioinformatics) who are interested in participating ongoing research activities. Please contact by email, stating how your expertise will be helpful for our research.

Current members
Mr. Gajanan Mehetre (CSIR-JRF, AcSIR Jan 2013 Batch)
Ms. Vidya Sonalkar
Ms. Garima Bhadauriya (Dissertation student, Apr-June 2014, M.Sc. Biotechnology,@ Jiwaji University, Gwalior, M.P. India)

Ms. Neelu Soni (Dissertation student
Dec2012-May2013, M.Tech. Biotechnology, @ D.Y. Patil, Mumbai, M.S., India)
Ms. Pratiksha Naik Bholnekar
(Dissertation student, Jan-May 2013, M.Sc. Biotechnology,  @ Dr. D.Y. Patil, Akurdi, Pune, M.S.,India)
Mr. Nilesh Kolhe (Project Assistant II; in NCIMRC project, Jan2013-Mar2014, M.Sc. Microbiology @University of Pune, Pune,M.S., India)
Mr. Yogesh Kumbhar (Dissertation student, June2013-Mar 2014, M.Sc. Microbiology@ Modern College, Ganeshkhind, Pune,M.S., India)

Funding in laboratory:
CSIR-NCL start-up grant (2012-2014)
CSIR- 12th FYP grant (2012-2017)
DST-SERB YS grant (2014-2016)

Other links

Publications: (

Research papers (PubMed citations- DHARNE M)

19. Kulkarni G, Dhotre D, Dharne M, Shetty S, Chowdhury S, Misra V, Misra S, Patole M, Shouche Y. Draft genome of Ochrobactrum intermedium strain M86 isolated from non-ulcer dyspeptic individual from India. Gut pathog. 2013 Apr 4;5(1):7. 

18. Dastager S, Krishnamoorthy S., Ramesh K, Dharne M. (In Press). Prokaryotes, 5th edition, Chapter Micrococcaceae. (Book chapter)

17. Bhagwat A, Kannan P, Leow Y, Dharne M, Smith A. Role of anionic charges of osmoregulated periplasmic glucans of Salmonella enterica serovar Typhimurium SL1344 in mice virulence. Arch Microbiol. 2012 Jun;194(6):541-8.

16. Gupta AK, Dharne M, Rangrez AY, Verma P, Ghate HV, Rohde M, Patole MS, Shouche YS. Ignatzschineria indica sp. nov. and Ignatzschineria ureaclastica sp. nov., isolated from adult flesh fly (Diptera: Sarcophagidae). Int J Syst Evol Microbiol. 2011 Jun;61(Pt 6):1360-9 

15.Liu L, Dharne M, Kannan P,Smith A,Meng J, Fan M, Boren TL,Ranallo RT,Bhagwat AA. Osmoregulated periplasmic glucans synthesis gene family of Shigella flexneri. Arch. Microbiol. 2010Mar;192(3):167- 74.

14. Kannan P, Dharne M, Smith A, Karns J, Bhagwat AA. Motility revertants of opgGH mutants of Salmonella enterica serovar Typhimurium remain defective in mice virulence. Curr Microbiol.  2009 Dec 59:641-5.

13Bhagwat AA, Jun W, Liu Liu, Kannan P, Dharne M, Pheh B, Tall BD, Kothary MH, Gross KC, Angle S, Meng J, Smith A. Osmoregulated periplasmic glucans of Salmonella enterica serovar Typhimurium are required for optimal virulence in mice. Microbiology. 2009 Jan;155(Pt 1):229-37.

12Hasan S, S Singh, RY Parikh, Dharne M , Patole MS , Prasad BLV, Shouche YS. Bacterial Synthesis of Copper/Copper Oxide Nanoparticles. J. Nanosci. Nanotechnol. 2008 ,8 (6) : 3191–3196.

11. Gower DJ, Giri V, Dharne M, Shouche YS. Frequency of independent origins of viviparity among caecilians (Gymnophiona): evidence from the first 'live-bearing' Asian amphibian. J Evol. Biol. 2008,21(5):1220-6.

10. Dharne M , Misra SP , Misra V, Dwivedi M , Patole MS , Shouche YS.  Isolation of urease positive Ochrobactrum intermedium in the stomach of non-ulcer dyspeptic patient from north India. J Microbiol. Immunol. infect. 2008,41(2):183-186.

9. Dharne M , Gupta A, Rangrez A, Ghate H, Patole M, Shouche Y. Antibacterial activities of multi drug resistant Myroides odoratimimus bacteria isolated adult flesh flies (Diptera : Sarcophagidae) are independent of metallo beta-lactamase gene. Brazillian J Microbiol. (ISSN 1517-8382). 2008, 39.

8. Bauer A, VB Giri, E. Greenbaum, TR Jackman, Dharne M, YS Shouche. On the Systematics of the Gekkonid Genus Teratolepis Günther, 1869: Another One Bites the Dust. Hamadryad. 2008,32, 2.

7. Pandey PK, Dhotre DP, Dharne M, Khadse AN, Hiremath UI, Chaudhari RD , Patole MS and Shouche YS. Conservation and Evolutionary Genetics of Indian Leopard, Panthera pardus fusca as studied by mitochondrial 12S rRNA gene sequence analysis. Current Science, 2007, 92; 8:25. 

6. Gower DJ, Dharne M, Bhatta G , Giri V, Vyas R , Govindappa V, Oommen OV, George J , Shouche Y, and Mark Wilkinson.. Remarkable genetic homogeneity in unstriped, long-tailed Ichthyophis (Amphibia: Gymnophiona: Ichthyophiidae) along 1500 km of the Western Ghats, India. J Zoology (London) 2007, 272 (3); 266-275.

5. Dharne M, Munot HP, Pujari R, Kakrani AL, Patole MS, Shouche YS. Helicobacter pylori cagA, vacA and iceA genotypes in Western Indian population of  Maharashtra with varied gastroduodenal diseases. Indian J Pathol. Microbiol. 2007,50(4):740.

4. Misra V, Misra SP, Dwivedi M, Shouche Y, Dharne M, Singh PA. H pylori in areas of gastric metaplasia in gall bladder and isolation. of H.pylori DNA from gallstones. Pathology 2007, 39 (4) :419-24.

3. Rastogi G*, Dharne M*, Kumar A, Valujkar S, Patole M, Shouche Y. Species identification and authentication of tissues of animal origin using mitochondrial and nuclear markers: implications to meat adulteration and endangered species trading. Meat Science (Elsevier) 2007. 76 (4) 666-674.

2. Dharne M, Patole M, Shouche Y. Microbiology of the insect gut: tales from mosquitoes and bees. J. Biosci. 31(3), 2006.

1. Rastogi G, Dharne M, Bharde A, Pandav VS, Ghumatkar SV, Krishnamurthy R, Patole M, Shouche Y. Species determination and authentication of meat samples by mitochondrial 12S rRNA gene sequence analysis and conformation sensitive gel electrophoresis. Current Science. 2004.87: 1278-1281.

Review/ minireview/news/clipboards

Rastogi G, Dharne M, Patole M, Shouche Y, Genomic test beefs up meat purity standards, 2004. News article in Nature News,  India Edition.

Equal contribution

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