Welcome to Microbiome Laboratory at NCL, India

Head, National Collection of Industrial Microorganisms (NCIM)
Biochemical Sciences Division
National Chemical Laboratory (CSIR-NCL)
Dr. Homi Bhabha Road, Pashan
Pune 411 008, Maharashtra, INDIA
E Mail- msdharne@gmail.com Alt- ms.dharne@ncl.res.in 

Phone (Lab) +91-20-25902456;Fax- +91-20-25902671

Research areas- Microbiology and Biotechnology

Specific interests- OMICS and microorganisms, Microbial Bioprospecting, Human gut microbiome, Microbial resources
Publications: (Link to NCBI- PubMed)

Research papers (PubMed citations- DHARNE M)


28.Navale GR, Rout CS, Gohil KN, Dharne M, Late DJ, Shinde SS. Oxidative and Membrane stress-mediated antibacterial activity of WS2 and rGO-WS2 Nanosheets. 2015. RSC Advances. In Press.

27. Nagoba BS, Dhotre S, Dharne M, Potdar S, Suklikar J, Suryawanshi S. Viridians group streptococci in patients undergoing tooth extraction. European J Gen Medicine. 2015. In Press.  DOI:10.15197/sabad.1.12.48.

26. Navale GR, Dharne M, Shinde SS. Antibiofilm Activity of tert-BuOH Functionalized Ionic Liquids with Methylsulfonate counteranion. 2015. RSC Advances. In press.

25. Ganesh M, Sheikh NK, Shah P, Mehetre G, Dharne MS, Nagoba BS. Detection of Clostridium tetani in human clinical samples using tetX specific primers targeting the neurotoxin. J Infect Public Health. 2015 Jul 25. pii: S1876-0341(15)00141-0. doi: 10.1016/j.jiph.2015.06.014.

24. Dharne M, Kannan P, Murphy C, Smith AD and Bhagwat AA. Swarm and swim motilities of Salmonella enterica serovar Typhimurium and role of osmoregulated periplasmic glucans. Microbiol Discov. 2015; 3:3. DOI: 10.7243/2052-6180-3-3.

23. Philem DP, Sonalkar VV, Dharne M, Prabhune AA. Purification and partial characterization of novel Penicillin V acylase from Acinetobacter sp. AP24 – isolated from Loktak Lake; an Indo-Burma biodiversity hotspot. 2015.  Preparative Biochemistry & Biotechnology. In Press.

22. Kulkarni G, Shetty S, Dharne M, Shouche Y. Genome sequencing analysis reveals virulence related gene content of Ochrobactrum intermedium strain 229E, a urease positive strain isolated from human gastric niche. FEMS Microbiol Lett. 2014 Oct;359(1):12-5. doi: 10.1111/1574-6968.12549.

21. Dhotre SV, Mehetre GT, Dharne MS, Suryawanshi NM, Nagoba BS. Isolation of Streptococcus tigurinus - a novel member of Streptococcus mitis group from a case of periodontitis. FEMS Microbiol Lett 2014 Aug;357(2):131-5. doi: 10.1111/1574-6968.

20. Kulkarni G, Dhotre D, Dharne M, Shetty S, Chowdhury S, Misra V, Misra S, Patole M, Shouche Y. Draft genome of Ochrobactrum intermedium strain M86 isolated from non-ulcer dyspeptic individual from India. Gut pathog. 2013 Apr 4;5(1):7. doi: 10.1186/1757-4749-5-7.

19. Dastager S, Krishnamoorthy S., Ramesh K, Dharne M. (In Press). Prokaryotes, 5th edition, Chapter Micrococcaceae. (Book chapter)

18. Bhagwat A, Kannan P, Leow Y, Dharne M, Smith A. Role of anionic charges of osmoregulated periplasmic glucans of Salmonella enterica serovar Typhimurium SL1344 in mice virulence. Arch Microbiol. 2012 Jun;194(6):541-8. doi: 10.1007/s00203-012-0791-4.

17. Bhagwat AA, Leow YN, Liu L, Dharne M, Kannan P. Role of Anionic Charges of Periplasmic Glucans of Shigella flexneri in Overcoming Detergent Stress. Foodborne Pathog Dis. 2012. Jul ; 9(7):632-7.doi: 10.1089/fpd.2011.1090.

16. Gupta AK, Dharne M, Rangrez AY, Verma P, Ghate HV, Rohde M, Patole MS, Shouche YS. Ignatzschineria indica sp. nov. and Ignatzschineria ureaclastica sp. nov., isolated from adult flesh fly (Diptera: Sarcophagidae). Int J Syst Evol Microbiol. 2011 Jun;61(Pt 6):1360-9. doi: 10.1099/ijs.0.018622-0. 

15.Liu L, Dharne M, Kannan P,Smith A,Meng J, Fan M, Boren TL,Ranallo RT, Bhagwat AA. Osmoregulated periplasmic glucans synthesis gene family of Shigella flexneri. Arch. Microbiol. 2010Mar;192(3):167- 74. doi: 10.1007/s00203-009-0538-z.

14. Kannan P, Dharne M, Smith A, Karns J, Bhagwat AA. Motility revertants of opgGH mutants of Salmonella enterica serovar Typhimurium remain defective in mice virulence. Curr Microbiol.  2009 Dec 59:641-5. doi: 10.1007/s00284-009-9486-8.

13. Bhagwat AA, Jun W, Liu Liu, Kannan P, Dharne M, Pheh B, Tall BD, Kothary MH, Gross KC, Angle S, Meng J, Smith A. Osmoregulated periplasmic glucans of Salmonella enterica serovar Typhimurium are required for optimal virulence in mice. Microbiology. 2009 Jan;155(Pt 1):229-37. doi: 10.1099/mic.0.023747-0.

12. Hasan S, S Singh, RY Parikh, Dharne M , Patole MS , Prasad BLV, Shouche YS. Bacterial Synthesis of Copper/Copper Oxide Nanoparticles. J. Nanosci. Nanotechnol. 2008 ,8 (6) : 3191–3196.

11. Gower DJ, Giri V, Dharne M, Shouche YS. Frequency of independent origins of viviparity among caecilians (Gymnophiona): evidence from the first 'live-bearing' Asian amphibian. J Evol. Biol. 2008,21(5):1220-6.
doi: 10.1111/j.1420-9101.2008.01577.x.

10. Dharne M , Misra SP , Misra V, Dwivedi M , Patole MS , Shouche YS.  Isolation of urease positive Ochrobactrum intermedium in the stomach of non-ulcer dyspeptic patient from north India. J Microbiol. Immunol. infect. 2008,41(2):183-186.

9. Dharne M , Gupta A, Rangrez A, Ghate H, Patole M, Shouche Y. Antibacterial activities of multi drug resistant Myroides odoratimimus bacteria isolated adult flesh flies (Diptera : Sarcophagidae) are independent of metallo beta-lactamase gene. Brazillian J Microbiol. (ISSN 1517-8382). 2008, 39.
doi: 10.1590/S1517-838220080002000035.

8. Bauer A, VB Giri, E. Greenbaum, TR Jackman, Dharne M, YS Shouche. On the Systematics of the Gekkonid Genus Teratolepis Günther, 1869: Another One Bites the Dust. Hamadryad. 2008,32, 2.

7. Pandey PK, Dhotre DP, Dharne M, Khadse AN, Hiremath UI, Chaudhari RD , Patole MS and Shouche YS. Conservation and Evolutionary Genetics of Indian Leopard, Panthera pardus fusca as studied by mitochondrial 12S rRNA gene sequence analysis. Current Science, 2007, 92; 8:25. 

6. Gower DJ, Dharne M, Bhatta G , Giri V, Vyas R , Govindappa V, Oommen OV, George J , Shouche Y, and Mark Wilkinson.. Remarkable genetic homogeneity in unstriped, long-tailed Ichthyophis (Amphibia: Gymnophiona: Ichthyophiidae) along 1500 km of the Western Ghats, India. J Zoology (London) 2007, 272 (3); 266-275.

5. Dharne M, Munot HP, Pujari R, Kakrani AL, Patole MS, Shouche YS. Helicobacter pylori cagA, vacA and iceA genotypes in Western Indian population of  Maharashtra with varied gastroduodenal diseases. Indian J Pathol. Microbiol. 2007,50(4):740.

4. Misra V, Misra SP, Dwivedi M, Shouche Y, Dharne M, Singh PA. H pylori in areas of gastric metaplasia in gall bladder and isolation. of H.pylori DNA from gallstones. Pathology 2007, 39 (4) :419-24.

3. Rastogi G*, Dharne M*, Kumar A, Valujkar S, Patole M, Shouche Y. Species identification and authentication of tissues of animal origin using mitochondrial and nuclear markers: implications to meat adulteration and endangered species trading. Meat Science (Elsevier) 2007. 76 (4) 666-674.

2. Dharne M, Patole M, Shouche Y. Microbiology of the insect gut: tales from mosquitoes and bees. J. Biosci. 31(3), 2006.

1. Rastogi G, Dharne M, Bharde A, Pandav VS, Ghumatkar SV, Krishnamurthy R, Patole M, Shouche Y. Species determination and authentication of meat samples by mitochondrial 12S rRNA gene sequence analysis and conformation sensitive gel electrophoresis. Current Science. 2004.87: 1278-1281.

Equal contribution

Current members:

Dr. MAHESH DHARNE (Group leader and PI)

-Dr. Monika Dhote (CSIR-RA), From Oct 2014, Ph.D. Biotechnology, CSIR-NEERI, Nagpur, India

-Mr. Gajanan Mehetre (CSIR-JRF), From Jan 2013, M.Sc. Biotechnology, Dr. BAMU, Aurangabad, Ind.
-Mr. Kushal Gohil (CSIR-JRF), From Aug 2014, M.Sc. Microbiology, SPU, Anand, India
-Ms. Priya Neurgaonkar (PAII), From Nov 2014, M.Sc. Microbiology, University of Pune, Pune, India
-Ms. Tejali Naik (PA-II), From June 2015, M.Sc. Microbiology, University of Pune, Pune, India

-Mr. Nilesh Kolhe (PAII) Jan 2013 to Mar 2014, M.Sc. Microbiology, University of Pune, India.
-Ms. Vidya Sonalkar (PA II), Jan 2013- May 2015, M.Sc. Microbiology, SRTMU, Nanded, India
-Mrs. Sneha Karia (PAII), June 2014- Mar 2015, M.Sc. Microbiology, RDU, Jabalpur, India

Open positions (email queries will be only entertained)
Please contact for dissertation project (minimum 5 months, full time)
Based on funding availability, I support the candidates having M.Sc. Microbiology, Biotechnology, Bioinformatics for CSIR, ICMR, DBT, INSPIRE fellowships.

Funding in laboratory:
CSIR India- 12th FYP grant (2012-2017)
DST-SERB, India- YS grant (2014-2016)
SERB, India Special Grant (2015-2018)

Past grants
CSIR-NCL start-up grant (2012-2014)

Links to my labpages at NCL