The FV1000 is an Olympus IX81 Inverted Microscope with widefield fluorescence visualization, brightfield and differential interference contrast. The system allows for four channel spectral and filter-based confocal scanning including transmitted light channel for DIC. This feature provides sensitivity and flexibility for imaging with and without fluorescent fluorophores and will allow users to image up to four compatible fluorophores simultaneously. We have 10X, 20X, 40X, and 100X objectives for use on this microscope.
A key feature of this instrument that was not available from other manufacturers is the incorporation of a second set of galvanometer mirrors for simultaneous photo-activation/stimulation/bleaching during imaging experiments. The second scan unit is a laser point scanner with the capacity to target defined regions using a Tornado (helical) scan. This feature allows activation and depletion of fluorescent probes for analysis of temporal and spatial activity in live samples.
This spectral detector based PMT system allows for specific bandwidth selection in colocalization experiments plus spectral scanning and separation of overlapping fluorophores. The FV1000 also has a motorized, programmable stage that can be used for multipoint timelapse and mosaic imaging. The six line laser combiner with AOTF control includes the following lines that will provide us the flexibility required to look at multiple fluorophores:
405 DAPI, Hoescht, Alexa 405
mArgon (457nm, 488nm, 514nm) CFP, GFP, FITC, Alexa 488, YFP
559 TRITC, CY3, mCherry, DsRed
635 Cy5, Alexa 647
Other features that this model includes multimode imaging in six dimensions including: XYZ, time, wavelength, mosaic scanning, and multipoint high content imaging.The Fluorescence Recovery after Photobleaching (FRAP) and Photoactivation (PA) that is facilitated by the easy software wizard with any laser line. The system also includes colocalization software module for complete analysis including scattergrams and statistical data ready for easy export.