S.pombe resources 


 
                                   ,-----archaeascomycetes-----------Schizosaccharomyces pombe
                                   |
                                   |                                                 ,------Saccharomyces cerevisiae
              ascomycete   |                                       ,----|
                 fungi-----|                                       |        `------Candida albicans
                                   |     
,--(budding) yeasts-|
                                   |     | (hemiascomycetes) `-----------Pichia pastoris
                                   `--|
                                         |                                               ,---Neurospora crassa
                                         `--filamentous ascomycetes-|
                                                  (euascomycetes)             |   ,-Aspergillus nidulans
                                                                                         `-|
                                                                                             `-Penicillium chrysogenum

 

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The fission yeast: Schizosaccharomyces pombe

S.pombe is phylogenetically as distant from budding yeasts as it is from humans. The Schizosaccharomyces lineage separated about 1 billion years ago to form the archaeascomycetes, an ancestral branch of the ascomycetes (see description and illustration by B. Franz Lang, University of Montreal).

 

Doubling time: 120m (30oC, YEA medium)

Homothallic strains are stable as haploid or diploid

Two mating types: P (plus) and M (minus)

Genome: 3 Chromosomes (1.38 x 10exp6 base pairs)

≈4900 genes, 43% with introns

Homologous Recombination is efficient but generally requires longer flanking homology than S.cerevisiae. With ≥50bp of 5' and 3' homologous flanking sequence it is possible to target a cassette to the appropriate genomic location with >50% accuracy

Readily available selection of stable plasmids (although high copy, which makes them less useful). These can be maintained by auxotrophic selection (eg. URA, LEU or ADE) or antibiotic resistance (eg. G418, Hygromycin, or Nat)

S.cerevisiae (UCD-522), 400x

 

S.pombe (131), 400x

Both images from Petrucchi library