S. cerevisiae resources 


 
                                   ,-----archaeascomycetes-----------Schizosaccharomyces pombe
                                   |
                                   |                                       ,------Saccharomyces cerevisiae
              ascomycete     |                                 ,----|
                 fungi---------|                                 |      `------Candida albicans
                                   |    
,---(budding) yeasts-|
                                   |   | (hemiascomycetes) `-----------Pichia pastoris
                                   `--|
                                       |                                      ,---Neurospora crassa
                                       `--filamentous ascomycetes-|
                                                  (euascomycetes)     |   ,-Aspergillus nidulans
                                                                                  `-|
                                                                                     `-Penicillium chrysogenum
 

Links

Genome Resources
  • geneDB : Sanger Institute sequencing

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Other Resources

The budding yeast: Saccharomyces cerevisiae

 Doubling time: 90m (30oC, YPD medium)

Two mating types: A and alpha

>99.9% of commonly used lab strains are heterothallic, deleted for the HO endonuclease, and stable as haploids or diploids. Homothallic strains (which contain HO) are infrequently used as they are stable only as diploids.

Genome: 16 Chromosomes (1.2 x 10exp6 base pairs)

≈6000 genes, with ≈4800 non-essential

Homologous Recombination is extremely efficient (with ≥50bp of 5' and 3' homologous flanking sequence it is possible to target a cassette to the appropriate genomic location with >90% accuracy)

Readily available selection of stable plasmids (CEN/ARS, single copy; 2micron, high copy). These can be maintained by auxotrophic selection (eg. HIS, TRP, URA, LEU, ADE or LYS; see also Pronk review) or antibiotic resistance (eg. G418 or Nat)

Less than 5% of S.cer genes contain introns (with only a handful containing more than one) although introns are well represented in the total RNA pool (many of the ribosomal protein-encoding genes have introns and these are transcribed at relatively high levels)

See: An Introduction to the Genetics and Molecular Biology of the
Yeast Saccharomyces cerevisiae

S.cerevisiae has been an amazingly productive model organism, at the forefront of many genomics and proteomic technologies. Readily available resources include gene deletion, epitope tag, overexpression, and GFP-tagged strain collections (for a review see: Suter et al (2006) Biotechniques 40:625).

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3 issues of Methods in Enzymology are available that cover most everything you'd ever need to do with budding yeast. All are edited by Christine Guthrie and Gerald R. Fink (apologies but the links only give you access to the full text if you have a licence)

Vol 194 (1991): Guide to Yeast Genetics and Molecular Biology

Vol 350 (2002): Guide to Yeast Genetics and Molecular Biology: Part B

 Vol 351 (2002): Guide to Yeast Genetics and Molecular Biology: Part C

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 S.cerevisiae with E.coli  for size comparison (2000x)