Publications and Research Interest

 

Mahesh's Biography

Mahesh Uttamchandani heads the Screening and Diagnostic Technologies Lab at the Defence Medical and Environmental Research Institute, DSO National Laboratories, Singapore. He earned his Ph.D. at the National University of Singapore (NUS) in 2007, following a B.Sc (Hons) in Microbiology in 2004. He also serves as an Adjunct Assistant Professor at the Departments of Chemistry and Biological Sciences, Faculty of Science, and the University Scholars Programme, NUS. 


Mahesh has published 50 peer-reviewed articles in international journals, 9 book chapters and edited a book on Small Molecule Microarrays. He has been cited over 1300 times and has a h-index of 23. He serves as a peer reviewer for many journals in the areas of peptide, protein and small molecule microarrays, high-throughput screening and rapid genotyping technologies. 


Mahesh teaches undergraduate and graduate courses in medicinal chemistry and molecular biology at NUS. He is a member of the American Chemical Society, the Royal Society of Chemistry and the Temasek Society. 


Mahesh has won the A*STAR/SNAS Young Scientist Award, the Lee Kuan Yew Global Business Plan competition, the Wang Gungwu Medal and Prize and the Sung Kah Kay memorial prize for his entrepreneurial and scientific achievements. He has also co-organized the annual Singapore Junior Chemistry Olympiad since its inception in 2010.

 

Research Interests

There are four broad areas that we are interested in, namely:

1) Proteomics/ Functional Genomics

  • Novel strategies in developing small molecule, peptide and protein microarrays for high-throughput applications
  • Developing tools for large-scale proteomics
  • High-throughput enzyme screening systems
  • Kinase, protease and phosphatase profiling

2) Peptide and Protein Engineering

  • Solid phase peptide synthesis
  • Combinatorial chemistry
  • Ribosome/Phage display

3) High-Throughput Genotyping

  • Developing strategies to rapidly profile multiple single nucleotide polymorphisms (SNPs)
  • Developing genotyping platforms on microarrays and bead-arrays.

4) Human Disease Genetics

  • Studying polymorphisms that predispose individuals to hypertension, obesity and cardiovascular disorders
  • Investigating genetic causes of sudden cardiac deaths








Publication History

(with DSO National Laboratories, as corresponding PI*)            Times Cited: 1297 (ISI WOS as of 9/6/2013)     H-Index: 23


(2013)

1.       Fu, J., Na, Z., Uttamchandani, M.,* Yao, S. Q.* “Profiling human Src homology 2 (SH2) domain containing proteins and ligand discovery using a small molecule microarray”,  2013, in preparation.

2.    Kalidasan, K., Su, Y., Wu, X., Yao, S. Q.,* Uttamchandani, M.* “Fluorescence-Activated Cell Sorting and Directed Evolution of α-N-Acetylgalactosaminidases Using a Quenched Activity-Based Probe (qABP)”, 2013, Chem. Comm. submitted.

3.    Uttamchandani, M.* “High-Throughput Screening: What are we missing?”, J. Biochips and Tissue Chips, 2013, e120.

4.    Kalidasan, K., Neo, J. L., Uttamchandani, M.* “Direct Visual Detection of Salmonella Genomic DNA using Gold Nanoparticles”, Mol. Biosyst. 2013, 9, 618-621.

5.     Wasan, P. S., Uttamchandani, M., Moochhala, S., Yap, V. B., Yap, P. H., “Application of Statistics and Machine Learning for Risk Stratification of Cardiac Arrhythmias”, Expert Syst. Appl. 2013, 40, 2476-2486.

 

(2012)   5 in total 

6.       Na, Z., Li, L., Uttamchandani, M.,* Yao, S. Q.* “Microarray-guided discovery of two-photon (2P) small molecule probes for live-cell imaging of cysteinyl cathepsin activities”, Chem. Comm. 2012, 48, 7304-7306.

7.       Thangavelu, K., Pan, Q. C., Balaji, G., Uttamchandani, M., Suresh, V., Karlberg, T., Low, B. C., Sivaraman, J., “Inhibitory Mechanism of Human Kidney Type Glutaminase (KGA) and its Synergism with MEK-2 in Cancer Cell Metabolism”, Proc. Natl. Acad. Sci. (USA) 2012, 109, 7705-7710. 

8.       Neo, J. L., Kalidasan, K., Uttamchandani, M.* “G-Quadruplex-based Probes for Visual DNA Detection”, Curr. Pharm Des. 2012, 18, 2048-2057.

9.       Gao, L. Uttamchandani, M.,* Yao, S. Q.* “Comparative Proteomic Profiling of Mammalian Cell Lysates Using Phosphopeptide Microarrays”, Chem. Comm. 2012, 48, 2240-2242.

10.       Foong, Y. M., Fu, J., Yao, S. Q.,* Uttamchandani, M.* “Current Advances in Peptide and Small Molecule Microarray Technologies”, Curr. Opin. Chem. Biol. 2012, 16, 234-242.

 

(2011)   5 in total

 

11.       Hu, M., Su, Y., Li, L., Wang, J., Yang, P.-Y., Uttamchandani, M., Xu, Q.-H., Yao S. Q. “Multi-Color, One- and Two- Photon Imaging of Enzymatic Activities in Living Cells with Quenched Activity-Based Probes (qABPs)” J. Am. Chem. Soc. 2011, 133, 12009-12020. 

12.       Shi, H. B., Uttamchandani, M., Yao, S. Q. “Small Molecule Microarray (SMM)-Facilitated Proteomic Profiling of Aspartic Protease with Cocktails of Affinity Probes” Chemistry – An Asian Journal, 2011, 6, 2803-2815.

13.       Wu, H., Ge, J., Uttamchandani, M.,* Yao, S. Q.* “Small Molecule Microarrays: The First Decade and Beyond” Chem Comm, 2011, 47, 5664-5670.

14.       Neo, J. L., Aw, K. L. D., Uttamchandani, M.* “Visual SNP Genotyping using Asymmetric PCR and Split DNA Enzymes” Analyst. 2011, 136, 1569-1572.

15.    Wu, H., Ge, J., Yang, P. Y., Wang, J., Uttamchandani, M., Yao S. Q. “A Peptide Aldehyde Microarray for High-Throughput Profiling of Cellular Events” J. Am. Chem. Soc. 2011, 133, 1946-1954.

 

(2010)    3 in total   

 

16.    Uttamchandani, M.,* Moochhala, S. “Microarray-Based Enzyme Profiling – Recent Advances and Applications” Biointerphases, 2010, 5, FA 24-31. (Focus issue: Biointerphase Science in Singapore)

17.    Aw, K. L. D., Neo, J. L., Uttamchandani, M.* “Visual Detection of DNA from Salmonella and Mycobacterium using Split DNAzymes” Mol. BioSyst. 2010, 6, 792-794.

18.    Hegde, A., Uttamchandani, M., Moochhala, S., Bhatia, M. “Plasma Cytokine Profiles in Preprotachykinin Knockout Mice Subjected to Polymicrobial Sepsis” Mol. Med. 2010, 16, 45-52.  (Joint first authors)

 

(2009)    2 in total   

 

19.    Uttamchandani, M., Lu C.H.S., Yao, S. Q. “Next Generation Chemical Proteomic Tools for Rapid Enzyme Profiling” Acc. Chem. Res. 2009, 42, 1183-1192.

20.    Uttamchandani, M.,* Neo, J.L., Ong, N. Z. B., Moochhala, S. “Applications of Microarrays in Pathogen Detection and Biodefence” Trends Biotechnol. 2009, 27, 53-61.





























(2008)    4 in total                 

21.    Uttamchandani, M.,* Yao, S. Q. “Peptide Microarrays: Next Generation Biochips for Detection, Diagnostics and High-Throughput ScreeningCurr. Pharm. Des. 2008, 14, 2428-2438.

22.    Lu, C. H. S., Sun, H., Bakar, F. B. A., Uttamchandani, M., Zhou, W., Liou, Y.-C, Yao, S. Q. “Rapid Affinity-Based Fingerprinting of 14-3-3 Isoforms Using A Combinatorial Peptide Microarray” Angew. Chem. Int. Ed. Engl. 2008. 47, 7438-7441.

23.    Uttamchandani M., Li, J., Sun, H., Yao, S. Q. “Activity-Based Protein Profiling: New Developments and Directions in Functional Proteomics” ChemBioChem. 2008, 9, 667-675. 

24.    Sun, H., Lu, C. H. S., Uttamchandani, M., Xia, Y., Liou, Y.-C., Yao, S. Q., “Peptide Microarray for High-Throughput Determination of Phosphatase Specificity and Biology” Angew. Chem. Int. Ed. Engl. 2008, 47, 1698-1702.       

(with Yao Laboratories, NUS)

 (2007)   4 in total

25.    Lee, W. L., Li, J., Uttamchandani, M., Sun, H., Yao, S. Q. “Inhibitor Fingerprinting of Enzymes using Microplate and Microarray Platforms – An Enabling Technology in Catalomics” Nat. Protocols. 2007, 2, 2126-2138.

26.    Uttamchandani, M., Lee, W. L., Wang, J., Yao, S. Q. “Quantitative Inhibitor Fingerprinting of Metalloproteases using Small Molecule Microarrays” J. Am. Chem. Soc. 2007, 129, 13110-13117.

27.    Uttamchandani, M., Wang, J., Li, J., Hu, M., Sun, H., Chen, K. Y.-T., Liu, K., Yao, S. Q. “Inhibitor Fingerprinting of Matrix Metalloproteases using a Combinatorial Peptide Hydroxamate Library” J. Am. Chem. Soc. 2007, 129, 7848-7858.

28.    Uttamchandani, M., Liu, K., Panicker, R. C., Yao, S. Q. “Activity-Based Fingerprinting and Inhibitor Discovery of Cysteine Proteases in a Microarray” Chem. Comm. 2007, 1518-1520.


(2006)     7 in total

29.    Wang, J., Uttamchandani, M., Li, J., Hu, M., Yao, S. Q. ““Click” Synthesis of Small Molecule Probes for Activity-Based Fingerprinting of Matrix Metalloproteases” Chem. Comm. 2006, 36, 3783-3785. 

30.    Wang, J., Uttamchandani, M., Li, J., Hu, M., Yao, S. Q. “Rapid Assembly of Matrix Metalloproteases (MMP) Inhibitors Using Click Chemistry” Org. Lett. 2006, 8, 3821-3824. 

31.    Wang, J., Uttamchandani, M., Hong, Y., Yao, S. Q. “Applications of Microarrays with Special Tagged Libraries” QSAR Comb. Sci. 2006, 11, 1009-1019.  

32.    Hu, Y., Uttamchandani, M., Yao, S. Q. “Microarray: A Versatile Platform for High-Throughput Functional Proteomics”, Comb. Chem. High Throughput Screening2006, 9, 203-212.

33.    Srinivasen, R., Uttamchandani, M., Yao, S. Q. “Rapid Assembly and In Situ Screening of Bidentate Inhibitors of Protein Tyrosine Phosphatases (PTPs), Org. Lett. 2006, 8, 713-716.

34.    Uttamchandani, M., Wang, J., Yao, S.Q. “Protein and Small Molecule Microarrays: Powerful Tools for High-Throughput Proteomics” Mol. BioSyst. 2006, 2, 58-68.

35.    Wang, J., Uttamchandani, M., Sun, L.P., Yao, S.Q. “Activity-Based High-Throughput Profiling of Metalloprotease Inhibitors Using Small Molecule Microarrays” Chem. Comm. 2006, 7, 717-719.


(2005)     2 in total

36.    Uttamchandani, M., Huang, X., Chen, G. Y. J., Yao, S. Q. “Nanodroplet Profiling of Enzymatic Activity on Microarrays” Bioorg. Med. Chem. Lett. 2005, 15, 2135-2139.   

37.    Uttamchandani, M., Walsh, D. P., Yao, S. Q., Chang, Y. T. “Small Molecule Microarrays – Recent Advances and Applications” Curr. Opin. Chem. Biol. 2005, 9, 4-13. 


(2004)     2 in total

38.    Uttamchandani, M., Walsh, D.P., Khersonsky, S.M., Huang, X., Yao, S.Q., Chang, Y.T. “Microarrays of Tagged Combinatorial Triazine Libraries in the Discovery of Small Molecule Ligands of Human IgG” J. Comb. Chem. 2004, 6, 862-868.

39.    Uttamchandani, M., Huang, X., Chen, G.Y.J., Tan, L.P., Yao, S.Q. “Application of Microarrays in High-Throughput Enzyme Profiling” Mol. Biotechnol. 2004, 28, 227-239.















(2003)     8 in total

40.    Yeo, S. Y. D., Srinivasan, R., Uttamchandani, M., Chen, Y. J. G., Zhu, Q., Yao, S. Q. “Cell-permeable Small Molecule Probes for Site-Specific Labeling of Proteins”, Chem.Comm., 2003, 2870-2871.

41.    Uttamchandani, M., Chan, E.W.S., Chen, G.Y.J., Yao, S.Q. “Combinatorial Peptide Microarrays for the Rapid Determination of Kinase Specificity”, Bioorg. Med. Chem. Lett. 2003, 13, 2997-3000.  

42.    Zhu, Q., Uttamchandani, M., Li, D.B., Lesaicherre, M. L., Yao, S. Q. “Enzymatic Profiling System in a Small-Molecule Microarray”, Org. Lett. 2003, 5, 1257-1260.

43.    Chen, G.Y.J., Uttamchandani, M., Zhu, Q., Wang, G., Yao, S.Q. “Developing a Strategy for Activity-Based Detection of Enzymes in a Protein Microarray”, ChemBioChem. 2003, 4, 336-339.

44.    Wang, G., Uttamchandani, M., Chen, G. Y. J., Yao S.Q. “Solid-Phase Synthesis of Peptide Vinyl Sulfones as Potential Inhibitors and Activity-Based Probes of Cysteine Proteases”, Org. Lett. 2003, 5, 737-740.

45.    Zhu, Q., Li, D.B., Uttamchandani, M., Yao, S.Q. “Facile Synthesis of 7-amino-4-carbamoylmethyl coumarin (ACC)-Containing Solid Supports and their Corresponding Fluorogenic Protease Substrates”, Bioorg. Med. Chem. Lett. 2003, 13, 1033-1036.

46.    Uttamchandani, M., Chen, G. Y. J., Lesaicherre, M. L., Yao, S. Q. “Methods for Generating Peptide Arrays for High-Throughput Enzyme Screenings”, Methods Mol. Biol. 2003, 264, 191-205.

47.    Chen, G.Y.J., Uttamchandani, M., Lue, R.Y.P. Lesaicherre, M.L., Yao. S.Q. “Array-Based Technologies and their Applications in Proteomics”, Curr. Topics in Med. Chem. 2003, 3, 705-724.

(2002)     3 in total

48.    Lesaicherre, M. L., Uttamchandani, M., Chen, G. Y. J., Yao, S. Q. “Developing Site-Specific Immobilization Strategies of Peptides in a Microarray”, Bioorg. Med. Chem. Lett. 2002, 12,  2079-2083.

49.    Lesaicherre, M. L., Uttamchandani, M., Chen, G. Y. J., Yao, S. Q. “Antibody-Based Fluorescence Detection of Kinase Activity on a Peptide Array”, Bioorg. Med. Chem. Lett. 2002, 12,  2085-2088.

50.    Xie, G. Y., Uttamchandani, M., Chen, G. Y. J., Bu, X. Z., Lin, S. S., Wong, K. M., Yan, W. L., Yao, S. Q., Guo, Z. H. “Substrate Spectrum of Tyrocidine Thioesterase Probed with Randomized Peptide N-Acetylcysteamine Thioesters”, Bioorg. Med. Chem. Lett. 2002, 12, 989-992.

BOOKS

51.    “High-Throughput Methodologies for Systematic Enzyme Profiling” Uttamchandani, M.: Lambert Academic Press, Germany, June 2011.  (ISBN 978-3-8443-8575-5)

                                                        

 

52.    “Small Molecule Microarrays: Methods and Protocols” Eds. Yao S. Q. and Uttamchandani, M.: Methods in Molecular Biology, Humana Press, Springer NY, Oct 2010. (ISBN 978-1-60761-844-7)

 

     

 BOOK CHAPTERS
         53.       Neo, J. L., Uttamchandani, M.,* “Visual DNA Detection and SNP Genotyping using Asymmetric PCR and Split  
         DNA Enzymes”, Nucleic Acid Detection, MiMB, 2012, in press.

         54.       Gao, L., Sun, H., Uttamchandani, M.,* Yao S. Q.* “Phosphopeptide Microarrays for Comparative Proteomic  
         Profiling of Cellular Lysates” Eds. Zhou, M and Veenstra, T. Proteomics for Biomarker Discovery: Humana Press. 
         2013, 233-251.

      55.       Foong, Y. M., Uttamchandani, M.* “Developing Influenza Antigen Microarrays for Seroprofiling” Eds. Rajasekaran, K., Cary, J. W., James, J., Montesinos, E. Small Wonders: Peptides for Disease Control: ACS Books, 2011, 193-202.  

56.    Uttamchandani, M.,* Yao, S. Q. “The Expanding World of Small Molecule Microarrays” Eds. Uttamchandani M. and Yao S. Q. Small Molecule Microarrays: Methods and Protocols: Humana Press, 2010.

57.    Shi, H., Uttamchandani, M., Yao, S. Q., “A method for small molecule microarray (SMM)-based screening for the rapid discovery of affinity-based probes (AfBPs)” Eds. Uttamchandani M. and Yao S. Q. Small Molecule Microarrays: Methods and Protocols: Humana Press, 2010.

58.    Aw, K. L. D., Yao, S. Q., Uttamchandani, M.* “Nanodroplet Microarrays for High-Throughput Enzyme Profiling” Eds. Uttamchandani M. and Yao S. Q. Small Molecule Microarrays: Methods and Protocols: Humana Press, 2010.

59.    Uttamchandani, M.,* “High-Throughput Screening Using Small Molecule Microarrays” Ed. Chittur, S. V. Microarray Methods for Drug Discovery: Humana Press, 2010.








60.    Uttamchandani, M., Chen G. Y. J., Lesaicherre, M.L., Yao, S. Q. “Site-specific immobilization for the rapid detection of kinase activity on microarrays” Ed. Fung E.T. Protein Arrays: Humana Press, 2004.

61.    Lue, Y. P. R., Yeo, S. Y. D., Tan, L. P. Uttamchandani, M., Chen, G. Y. J., Yao, S. Q., “Novel strategies in protein microarray generation and detection” Ed. Schena M. Protein Microarray: Jones and Barlett Publishers, 2003.


PATENTS

1.       Kalidasan, K., Neo, J. L., Uttamchandani, M. “Visual Detection of Genomic DNA using Gold Nanoparticles” (2012) PCT filed, 23 Oct 2012.

2.       Lesaicherre, M. L., Lue, Y. P. R., Uttamchandani, M., Chen, G. Y. J., Yao, S. Q. “Intein-mediated attachment of ligands to proteins for immobilization onto a support” (2004) US Patent # 20040265921, USPTO Class 435007500, filed 30 June 2003, published 30 Dec 2004.

3.       Lesaicherre, M. L. et al. “Novel strategies for the site-specific immobilization of peptides and proteins onto glass slides”, submitted (2003)

4.       Uttamchandani M., Chen, G. Y. J., Yao, S. Q. “Novel methods for detecting enzymatic activity on a protein microarray”, submitted (2003)