Research

My research focuses on understanding the molecular processes of life, mechanistic biology, mechanisms of enzyme action, protein-protein/DNA interactions, biological electron transfer, energy and signal transduction, as well as how to apply this knowledge in medical science and biotechnology. In addition to in vitro work, I also investigate molecular interactions in living cells, in real time.

   

Research Methods and Aproaches

  • Gene Cloning & Protein Engineering
  • Protein Film Electrochemistry 
  • Fluorescent & Electrochemical Biosensing
  • Split Fluorescent Protein Technology
  • FRET & BiFC for Molecular Interactions
  • Total Internal Reflection Fluorescence Microscopy (TIRF)
  • Super-Resolution Fluorescence Microscopy (PALM)
    • super-resolution imaging
    • single molecule tracking
  • Applied Biophysics For Drug Discovery (SPR, BLI, MST)
    • affinity, kinetics, thermodynamics, stability



Single-molecule transcription factor dynamics in a living cell, kinetics of protein binding and unbinding from bacterial DNA



Chen, T.Y., Saniago, A.G., Jung, W., Krzeminski, L et al., Nature Communications, 2015


Electron transfer kinetics of turning-over metalloenzyme reported with electric current and fluorescence outputs



Krzeminski, L. et al., Journal of the American Chemical Society, 2011



  

Proof of principle of super-resolution bimolecular fluorescence complementation (BiFC) at the bacterial inner membrane,        an evidence of Tsr chemoreceptor clustering