Dr Nathan Le's Homepage

Hello, my name is Dr Nathan (Nam) Le. I am an Australian lawyer and also a registered migration agent (MARN 1910130) practising at Bardo Lawyers, an accredited specialist immigration law firm in Melbourne, Australia.

My contact details are as follows:

Bardo Lawyers

Level 24, 570 Bourke Street, Melbourne VIC 3000

Tel: +61 3 8658 5830

Fax: +61 3 9304 3366

Email: nathan@belawyers.com.au

Prior to practising law, I have assumed the following roles in a number of countries:

▪ Scientist at CSIRO Manufacturing and CSIRO Energy, Clayton, Victoria, Australia

▪ Post-doctoral Researcher at Biomedical Diagnostics Institute, Dublin, Ireland

▪ Graduate Research Assistant at Ritsumeikan University, Kyoto, Japan

▪ Research Engineer at NanoScience Innovation Pte. Ltd., Singapore

▪ Laboratory Technician at Aerodynamic Laboratory, Ho Chi Minh City University of Technology, Vietnam

Education

▪ LL.B. Bachelor of Laws (Hons) (Distinction, top 15% of the class)

Deakin University, Melbourne, Australia

▪ Ph.D. in Mechanical Engineering

Ritsumeikan University, Kyoto, Japan

▪ M.Sc. in Materials Science

Joint program between National University of Singapore, Singapore and Massachusetts Institute of Technology (MIT), Boston, MA, USA

▪ B.Eng. (Hons) in Aeronautical Engineering

Vietnam National University, Ho Chi Minh City University of Technology, Vietnam

Professional activities

▪ Member of Law Institute of Victoria (LIV), Victoria, Australia, 2018 -

▪ Member of the Institution of Engineers Australia (MIEAust), Australia, 2013 - 2014

▪ Reviewer for Measurement Science and Technology, Institute of Physics Publishing (IOP), 2009

▪ Reviewer for Journal of Micromechanics and Microengineering, Institute of Physics Publishing (IOP), 2011

▪ Reviewer for Analytical and Bioanalytical Chemistry, Springer publishing, 2011 and 2012

▪ Reviewer for Langmuir, American Chemical Society (ACS) publishing, 2012

▪ Reviewer for IEEE/ASME Journal of Microelectromechanical, IEEE publishing, 2013

Honors and awards

▪ Geelong Law Association prize, best student award in MLL111 Contract Law, 2015

▪ Member of Golden Key International Honour Society for being among the top 15% of the law cohort at Deakin University

▪ CSIRO fellowship (3 years) and CSIRO corporate citizen award

▪ Monbukagakusho scholarship for Ph.D student from Ministry of Education, Culture, Sports, Science and Technology of Japan (3.5 years)

▪ Scholarship from Singaporean Government to pursue master degree at SMA (1 year)

▪ Scholarship for excellent student from Dong-Ah Elecomm (Korea)

Intellectual Property (IP) and Publications

Patents

[1] Total reflection biochip for fluorescence microscope, manufacturing method thereof, and total reflection biochip assembly for fluorescence microscope (in Japanese) Japan patent application No.: 2008-119836, Japan patent publication No.: 2008-298-711, 2008

[2] A surface coating for biomolecule immobilisation and minimisation of non-specific binding on surfaces for biomedical diagnostics, US patent application No: 13/049,086, Filing date: March 16, 2011

[3] Methods and systems for detecting an analyte or classifying a sample, US patent application No: 61/624,899, Filing date: April 16, 2012, International application No.: PCT/AU2013/000378, Filing date: April 15, 2013, International publication No.: WO/2013/155553, Publication date: Oct. 24, 2013,

Trade Mark

[1] 1 Trade Mark (please contact me for details).

Book chapter (refereed)

[1] Murat Gel, Nam Cao Hoai Le, Alisha Anderson, Stephen Trowell, Yonggang Zhu “Bioluminescence and bioluminescence-resonance energy transfer assays in microfluidics” Encyclopedia of Microfluidics and Nanofluidics, D. Li (Ed.), 2014, pp. 1-8 DOI: 10.1007/978-3-642-27758-0_1765-4

Journal papers (refereed)

[1] Yanping Du, Christopher J. Fell, Yonggang Zhu, Nam Cao Hoai Le, Glenn Bradbury "Advanced thermal management of a solar cell by a nano-coated heat pipe plate: A thermal assessment" Energy Conversion and Management (Elsevier) 134 (2017), pp. 70-76 DOI: 10.1016/j.enconman.2017.06.081 DOI: 10.1016/j.enconman.2016.11.059

[2] Peter W. Akers, Nam Cao Hoai Le, Andrew R. J. Nelson, Milena McKenna, Christy O’Mahony, Duncan J. McGillivray, Vladimir Gubala, David E. Williams "Surface engineering of poly(methylmethacrylate): Effects on fluorescence immunoassay" Biointerphases (AIP) 12 (2017), 02C415 DOI: 10.1116/1.4984010

[3] Yanping Du, Nam Cao Hoai Le, Dong Chen, Huaying Chen, Yonggang Zhu "Thermal management of solar cells using a heat pipe plate: an indoor experimental study" International Journal of Energy Research, 41 (2017), pp. 867-876 DOI: 10.1002/er.3678

[4] Yinan Zhang, Yanping Du, Clifford Shum, Boyuan Cai, Nam Cao Hoai Le, Xi Chen, Benjamin Duck, Chris Fell, Yonggang Zhu, Min Gu "Efficiently-cooled plasmonic amorphous silicon solar cells integrated with a nano-coated heat-pipe plate" Scientific Reports 6 (2016) pp. 24972 DOI: 10.1038/srep24972

[5] Nam Cao Hoai Le*, Murat Gel, Yonggang Zhu, Jian Wang, Helen Dacres, Alisha Anderson, Stephen Trowell “Sub-nanomolar detection of thrombin activity on a microfluidic chip” Biomicrofluidics, 8, 2014, pp. 064110 DOI: 10.1063/1.4902908

[6] Nam Cao Hoai Le*, Mural Gel, Yonggang Zhu, Helen Dacres, Alisha Anderson, Stephen Trowell “Real-time, continuous detection of maltose using bioluminescence resonance energy transfer (BRET) on a microfluidic system” Biosensors and Bioelectronics, 62 (2014), pp. 177-181 DOI: 10.1016/j.bios.2014.06.032

[7] Nam Cao Hoai Le*, Dzung Viet Dao, Ryuji Yokokawa, Thien Duy Nguyen, John C. Wells and Susumu Sugiyama "Highly-sensitive fluorescence detection and imaging with microfabricated total internal reflection (TIR)-based devices" Journal of Micro-Nano Mechatronics, 7 (2012), pp. 45-59 (invited article) DOI: 10.1007/s12213-012-0043-3

[8] Nam Cao Hoai Le*, Vladimir Gubala, Eoin Clancy, Thomas Barry, Terry Smith, David E. Williams "Ultrathin and smooth poly(methyl methacrylate) (PMMA) films for label-free biomolecule detection with total internal reflection ellipsometry (TIRE)" Biosensors and Bioelectronics, 36 (2012), pp. 250-256 DOI: 10.1016/j.bios.2012.04.032

[9] Conor Coyle, Ram P. Gandiraman, Vladimir Gubala, Nam Cao Hoai Le, Christy Charlton O'Mahony, Colin Doyle, Bryony James, Paul Swift, Stephen Daniels, David E. Williams "Tetraethyl orthosilicate and acrylic acid forming robus carboxylic functionalities on plastic surfaces for biodiagnostics" Plasma Processes and Polymers, 9 (2012), pp. 28-36 DOI: 10.1002/ppap.201100070

[10] Ram P. Gandhiraman, Nam Cao Hoai Le, Colin Doyle, Cedric Volcke, Vladimir Gubala, Suresh Uppal, Chandra K. Dixit, Ruari Monaghan, Bryony James, Richard O’Kennedy, Stephen Daniels, David E. Williams "Multi-layered plasma-polymerized chips for SPR based detection" ACS Applied Materials and Interfaces, 3 (2011), pp. 4640-4648 DOI: 10.1021/am201061k

[11] Nam Cao Hoai Le*, Vladimir Gubala, Ram P. Gandhiraman, Stephen Daniels and David E. Williams "Evaluation of different non-specific binding blocking agents deposited inside poly(methyl methacrylate) (PMMA) microfluidic flow-cells" Langmuir, 27 (2011), pp. 9043-9051 DOI: 10.1021/la2011502

[12] Christy Charlton, Vladimir Gubala, Ram Prasad Gandhiraman, Julie Wiechecki, Nam Cao Hoai Le, Conor Coyle, Stephen Daniels, Brian D. MacCraith and David E. Williams "TIRF microscopy as a screening method for non-specific binding on surfaces" Journal of Colloid and Interface Science, 354 (2011), pp. 405-409 DOI: 10.1016/j.jcis.2010.10.029

[13] Nam Cao Hoai Le*, Vladimir Gubala, Ram P. Gandhiraman, Conor Coyle, Stephen Daniels and David E. Williams "Total internal reflection ellipsometry (TIRE) as a label-free assessment method for optimization of the reactive surface of bioassay devices based on a functionalized cyclo olefin polymer" Analytical and Bioanalytical Chemistry, 398 (2010), pp. 1927-1936 DOI: 10.1007/s00216-010-4099-4

[14] Vladimir Gubala, Nam Cao Hoai Le, Ram Prasad Gandhiraman, Conor Coyle, Stephen Daniels and David E. Williams "Functionalization of cyclo olefin polymer substrates by plasma oxidation: Stable film containing carboxylic acid groups for capturing biorecognition elements" Colloids and Surfaces B: Biointerfaces, 81 (2010), pp. 544-548 DOI: 10.1016/j.colsurfb.2010.07.055

[15] R.P. Gandhiraman, V. Gubala, Nam Cao Hoai Le, C. Volcke, C. Doyle, B. James, S. Daniels and D.E. Williams "Deposition of chemically reactive and repellent sites on biosensor chips for reduced non-specific binding" Colloids and Surfaces B: Biointerfaces, 79 (2010), pp. 270-275 and 84 (2011), pp. 589, DOI: 10.1016/j.colsurfb.2010.04.009 and DOI: 10.1016/j.colsurfb.2011.01.019

[16] Ryuji Yokokawa, Yoshihiro Manta, Moriaki Namura, Yusuke Takizawa, Nam Cao Hoai Le and Susumu Sugiyama "Individual evaluation of DEP, EP and AC-EOF effects on lambda-DNA molecules in a DNA concentrator" Sensors and Actuators B: Chemical, 143 (2010), pp. 769-775 DOI: 10.1016/j.snb.2009.10.025

[17] Nam Cao Hoai Le*, Dzung Viet Dao, Ryuji Yokokawa, John C. Wells and Susumu Sugiyama "A monolithic dual-color total-internal-reflection-based chip for highly sensitive and high-resolution dual-fluorescence imaging" IEEE/ASME Journal of Microelectromechanical Systems, 18 (2009), pp. 1371-1381 DOI: 10.1109/JMEMS.2009.2030938

[18] Nam Cao Hoai Le*, Dzung Viet Dao, Ryuji Yokokawa, John C. Wells and Susumu Sugiyama "Fabrication of optically smooth, through-wafer silicon molds for PDMS Total Internal Reflection (TIR)-based devices" Microsystem Technologies, 15 (2009), pp. 1845-1853 DOI: 10.1007/s00542-009-0913-3

[19] Nam Cao Hoai Le*, Ryuji Yokokawa, Dzung Viet Dao, Thien Duy Nguyen, John C. Wells and Susumu Sugiyama "Versatile microfluidic Total Internal Reflection (TIR)-based devices: Application to microbeads velocity measurement and single molecule detection with upright and inverted microscope" Lab on a Chip, 9 (2009), pp. 244-250 DOI: 10.1039/B807408A

This article was also selected for Virtual Journal of Biological Physics Research 17(2), American Institute of Physics (AIP) and the American Physical Society (APS), Jan 15th 2009

[20] Nam Cao Hoai Le*, Dzung Viet Dao, Ryuji Yokokawa, John Wells and Susumu Sugiyama "Design, simulation and fabrication of a Total Internal Reflection (TIR)-based chip for highly-sensitive fluorescent imaging" Journal of Micromechanics and Microengineering, 17 (2007), pp. 1139-1146 DOI: 10.1088/0960-1317/17/6/006

*Corresponding author

Conference presentations (refereed)

[1] Nam Cao Hoai Le, Glenn Bradbury, Yanping Du, Yonggang and Yonggang Zhu "Thin, flexible polymer-based heatpipes for flexible electronics" 7th Australia and New Zealand Nano-Microfluidics Symposium (ANZNMF), Brisbane, Australia, 21-23 March, 2016 (poster)

[2] Nam Cao Hoai Le*, M. Gel, Y. Zhu, H. Dacres, A. Anderson, S. Trowell “Real-time, microfluidic measurement of maltose concentration using a continuously flowing optical biosensor” 24th Anniversary World Congress on Biosensors, Melbourne, Australia, 27-30 May, 2014 (oral)

[3] Nam Cao Hoai Le, Murat Gel, Yonggang Zhu, Fiona Glenn, Helen Dacres, Alisha Anderson, Stephen Trowell "Fiber-based bioluminescence-resonance energy transfer (BRET) system on a microfluidic chip" The Third International Conference on Advances in Microfluidics and Nanofluidics (AMN2012), 23-26 May 2012, Dalian, China (invited oral)

[4] R. P. Gandhiraman, V. Gubala, N. C. H. Le, C. Charlton, C. Doyle, B. James, S. Daniels, D. E Williams "Plasma surface functionalisation of disposable plastics for point of care diagnostics" Fifth International Conference on Advanced Materials and Nanotechnology (AMN 5), 7-11 February 2011, Wellington, New Zealand (oral)

[5] N. C. H. Le, R. P. Gandhiraman, V. Gubala, S. Daniels, D. E. Williams "Surface treatment of poly(methyl methacrylate) (PMMA) microfluidic flow-cells for reducing non-specific adsorption of proteins" Fifth International Conference on Advanced Materials and Nanotechnology (AMN 5), 7-11 February 2011, Wellington, New Zealand (oral)

[6] V. Gubala, C. Lynam, A. Barnett, R. P. Gandhiraman, C. Charlton, N. C .H. Le, D. E. Williams "Recent advances in the surface chemistry of dye-doped nanoparticles and disposable, plastic substrates for highly sensitive and rapid bioassay devices" Fifth International Conference on Advanced Materials and Nanotechnology (AMN-5), 7-11 February 2011, Wellington, New Zealand (oral)

[7] R. Gandhiraman, V. Gubala, D. E. Williams, C. Lynam, C. O'Mahony, N. C. H. Le "Designing low sample volume, high-sensitivity, high-precision, single-use immunoassay devices" 20th Anniversary World Congress on Biosensors (Biosensors 2010), 26-28 May 2010, Glasgow, United Kingdom (poster)

[8] Nam Cao Hoai Le, Vladimir Gubala, Ram Prasad Gandhiraman, David E. Williams "Total Internal Reflection Ellipsometry (TIRE) for the Monitoring of Immunoassays on a Functionalized Cyclo-Olefin Copolymer Surface" Book of Abstracts of EUROPTRODE X 2010, pp. 70, Prague, Czech Republic, Mar. 28-31, 2010 (poster)

[9] Nam Cao Hoai Le, Ryuji Yokokawa, Dzung Viet Dao, John C. Wells and Susumu Sugiyama "Dual-color Total Internal Reflection (TIR)-based Devices with Uniform and High S/N Ratio overlapped Evanescent Fields" The Proceedings of 13th Int'l Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2009), pp. 1602-1604, ICC Jeju, Jeju, Korea, Nov. 1-5, 2009 (poster)

[10] Nam Cao Hoai Le, Dzung Viet Dao, Ryuji Yokokawa, John C. Wells and Susumu Sugiyama "All PDMS Multi-color Total Internal Reflection (TIR)-based Devices for Multi-fluorescence Detection and Imaging" Technical Digest of 8th IEEE Int'l on Sensors (IEEE SENSORS 2009), pp. 1636-1639, Christchurch, New Zealand, Oct. 25-28, 2009 (oral) DOI:10.1109/ICSENS.2009.5398506

[11] Nam Cao Hoai Le, Ryuji Yokokawa, Dzung Viet Dao, John C. Wells and Susumu Sugiyama "Dual-color Total Internal Reflection (TIR)-Based Devices with Smooth PDMS Lens for High S/N Ratio Overlapped Evanescent Fields" The Proceedings of 26th Sensor Symposium on Sensors, Micromachines and Applied Systems, pp. 413-418, Tokyo, Japan, Oct. 15-16, 2009 (poster)

[12] Moriaki Namura, Yoshihiro Manta, Yusuke Takizawa, Nam Cao Hoai Le, Susumu Sugiyama and Ryuji Yokokawa "DEP and EP Effects on AC-EOF in a DNA Concentrator" The Proceedings of 26th Sensor Symposium on Sensors, Micromachines and Applied Systems, pp. 413-418, Tokyo, Japan, Oct. 487-492, 2009 (oral)

[13] Nam Cao Hoai Le, Dzung Viet Dao, Ryuji Yokokawa, John Wells and Susumu Sugiyama "Monolithic Multicolor Total Internal Reflection (TIR)-based Chip for Highly-sensitive and Simultaneous Multifluorescence Imaging and Detection" The Proceedings 22nd IEEE Int'l Conference on Micro Electro Mechanical Systems (IEEE MEMS 2009), pp. 1011-1014, Sorrento, Italy, Jan. 25-29, 2009 (poster) DOI:10.1109/MEMSYS.2009.4805557

[14] Nam Cao Hoai Le, Dzung Viet Dao, Ryuji Yokokawa, John Wells and Susumu Sugiyama "Highly-sensitive Dual-fluorescence Detection and Imaging with Integrated Dual-color Total Internal Reflection (TIR)-based Chip" 2008 IEEE Int'l Symposium on Micro-Nano Mechatronics and Human Science (IEEE MHS 2008), pp. 27-32, Nagoya, Japan, Nov. 6-9, 2008 (oral) DOI:10.1109/MHS.2008.4752417

[15] Nam Cao Hoai Le, Ryuji Yokokawa, Dzung Viet Dao, John Wells and Susumu Sugiyama "Design, Analysis and Fabrication of a Dual-color Total Internal Reflection (TIR)-based Chip for Simultaneous Detection of Two Fluorophores" The Proceedings of 25th Sensor Symposium on Sensors, Micromachines and Applied Systems, pp. 137-140, Okinawa, Japan, Oct. 22-24, 2008 (oral)

[16] Nam Cao Hoai Le, Dzung Viet Dao, Ryuji Yokokawa, John Wells and Susumu Sugiyama "A Dual-color Total Internal Reflection (TIR)-based Chip for Simultaneous Detection of Two Fluorophores" Technical Digest of 7th IEEE Int'l Conference on Sensors (IEEE SENSORS 2008), pp. 1191-1194, Lecce, Italy, Oct. 26-29, 2008 (oral) DOI:10.1109/ICSENS.2008.4716655

[17] Nam Cao Hoai Le, Ryuji Yokokawa, Dzung Viet Dao, Thien Duy Nguyen, John Wells and Susumu Sugiyama "Integration of Evanescent Excitation (EE)-based Chip with Microfluidic Channels for Upright and Inverted Microscope Observations" The Proceedings of 12th Int'l Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2008), pp. 1603-1605, San Diego, CA, USA, Oct. 12-16, 2008 (poster)

[18] Nam Cao Hoai Le, Ryuji Yokokawa, Dzung Viet Dao, Thien Duy Nguyen, John Wells and Susumu Sugiyama "Application of an Integrated Microfluidic Total Internal Reflection (TIR)-based Chip to Nano-Particle Image Velocimetry (nano-PIV)" Technical Digest of 6th IEEE Int'l Conference on Sensors (IEEE SENSORS 2007), pp. 454-457, Atlanta, Georgia, USA, Oct. 28-31, 2007 (oral) DOI:10.1109/ICSENS.2007.4388434.

[19] Dzung Viet Dao, Nam Cao Hoai Le, Ryuji Yokokawa, John Wells and Susumu Sugiyama "A Monolithic Evanescent Excitation (EE)-Based Biochip for Highly-sensitive and Real-time Fluorescent Detection" The Proceedings of 11th Int'l Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2007), pp. 17-19, Paris, France, Oct. 7-11, 2007 (oral)

[20] Nam Cao Hoai Le, Dzung Viet Dao, Ryuji Yokokawa, John Wells and Susumu Sugiyama "A Total Internal Reflection (TIR)-based Chip for Ultra-sensitive Fluorescent Sensing" Digest of Technical Papers of 14th Int'l Conference on Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS'07), pp. 1781-1784, Lyon, France, Jun. 10-14, 2007 (poster) DOI: 10.1109/SENSOR.2007.4300499

[21] Nam Cao Hoai Le, Dzung Viet Dao, John Wells and Susumu Sugiyama "Design, Simulation and Fabrication of Monolithic Total Internal Reflection (TIR) Chip for Fluorescent-based Bio Detection" Proceedings of 2006 IEEE Int'l Symposium on Micro-Nano Mechatronics and Human Science (IEEE MHS 2006), pp. 30-34, Nagoya, Japan, Nov. 6-8, 2006 (oral) DOI: 10.1109/MHS.2006.320316

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