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Optical imaging has garnered significant interest due to its exceptional resolution, biocompatibility, affordability, and compact integration. It finds widespread applications not only in research fields like biology, medicine, and physics but also in industrial metrology, optical sensors, military visions, and more.

Among the various optical imaging modalities, optical microscopy has emerged as an indispensable tool, particularly in biology and medicine, allowing the visualization of vital phenomena occurring at the molecular level within tissues or single cells. Currently, many types of microscopes based on diverse physical principles, such as white light microscopy, phase-contrast microscopy, confocal microscopy, multip-photon microscopy, and others, are extensively employed to gather specific information about target samples.

In recent years, interferometric microscopy has emerged as a powerful technique for numerous applications. It enables the visualization of the phase delay experienced by light as it passes through an object. By employing the interferometric measurement technique known as digital holography, which captures both intensity and phase alterations of light, phase imaging offers precise mapping of physical properties like refractive index distribution, height (thickness), and dry mass of the target object. As living cells and tissues are predominantly transparent to visible light, conventional white light microscopes often provide vague and limited visibility of detailed structures. However, interferometric microscopy, by capturing refractive index distribution, produces sample images with significantly higher contrast. Thus, the interferometric imaging technique holds a distinct advantage for observing cells and tissues.

The Biophotonics Imaging Laboratory is committed to developing novel microscopic techniques that combine physical principles with state-of-the-art imaging technologies based on digital holography. Furthermore, we are actively addressing the limitations of resolution and imaging depth encountered in existing microscopy by developing a light control technique for deep tissue imaging. Our endeavors aim to enable the observation of new life phenomena that were previously inaccessible with conventional microscopes and contribute to the establishment of novel disease diagnosis protocols.